| 1. |
- Borgfeldt, Christer, et al.
(författare)
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High tumor tissue concentration of urokinase plasminogen activator receptor is associated with good prognosis in patients with ovarian cancer
- 2003
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136. ; 107:4, s. 658-665
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Tidskriftsartikel (refereegranskat)abstract
- volved in tumor growth and metastasis. We assayed the components of the uPA system in homogenates of 64 primary epithelial ovarian tumors and 5 metastases and evaluated the association of these parameters to prognosis in the 51 malignant cases. The levels of uPA, PAI-2 and the uPA: PAI-I complex increased with progressive loss of histological differentiation (P-trend <0.001, <0.05 and <0.001). The level of PAI-I was higher in poorly than in well/moderately differentiated tumors (p = 0.03). The content of uPAR was lower in benign tumors as compared to borderline malignancies (p = 0.002), invasive primary tumors (p < 0.001), and metastases (p = 0.002). Surprisingly, the level of uPAR was lower in poorly differentiated as compared to both borderline (p = 0.01) and well differentiated malignant tumors (p = 0.005). Also, the level of uPAR was lower in advanced as compared to early stages of the disease (P-trend = 0.002). The median follow-up time for patients was 5.8 years. High tumor tissue levels of uPAR were associated with longer postoperative survival (HR = 0.4, 95% CI = 0.2-0.8, p = 0.01). In contrast, shorter survival was evident in patients with high tumor levels of uPA from 2 years on after operation (HR = 4.6, 95% CI = 1.2-17, p = 0.02). High tPA levels tended to be associated with shorter overall survival after 2 years (HR = 2.9, 95% 95% Cl = 0.9-9.8, p = 0.08). Although high tumor tissue content of uPAR was associated with a less aggressive phenotype characterized by well differentiated histology and longer survival, low content of uPAR in the poorly differentiated tumors and metastases presumably results from increased elimination of uPAR. (C) 2003 Wiley-Liss, Inc.
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| 2. |
- Brun, Eva, et al.
(författare)
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Prognostic value of histopathological response to radiotherapy and microvessel density in oral squamous cell carcinomas
- 2001
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Ingår i: Acta Oncologica. - : Informa UK Limited. - 1651-226X .- 0284-186X. ; 40:4, s. 491-496
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Tidskriftsartikel (refereegranskat)abstract
- The prognostic value of histopathological response to preoperative radiotherapy (50 Gy) in radically resected oral carcinomas was studied in 39 consecutive patients. Microvessel density (MVD) was evaluated for relation to radioresponse and outcome. Resected tumour tissue was examined histopathologically and response to radiotherapy was scored according to induced morphological changes. Pretreatment biopsies were stained with antibodies to von Willebrand factor to evaluate MVD in hot-spot regions, in stromal tissue and in tumour epithelial tissue. Histopathological response to radiotherapy was highly prognostic of local failures and survival (p = 0.002), though microscopic surgical radicality was obtained. In good responders to preoperative radiotherapy, the 5-year survival rate was 68% compared with 24% in poor responders. In 12 patients with local recurrence after radical surgery, 11 had poor histopathological radiotherapy responses. In univariate analysis, a high MVD score in tumour epithelium was associated with poor clinical outcome but MVD did not correlate with histopathological radiotherapy response.
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| 3. |
- Dahlgren, Eva, et al.
(författare)
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Sertoli-Leydig cell tumour in a postmenopausal woman showing all facets of the insulin resistance syndrome (IRS)
- 2005
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Ingår i: Ups J Med Sci. - 0300-9734. ; 110:3, s. 233-6
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Tidskriftsartikel (refereegranskat)abstract
- Sertoli-Leydig cell tumours are rare sex stromal tumours with an incidence of < 0.5% of all ovarian tumours. Most frequently this tumour occurs in young women with a history of amenorrhoea, hirsutism and lowered pitch. Here, we report on a woman with IRS, postmenopausal virilization and increased testosterone levels due to a Sertoli-Leydig cell tumour. This is the first case to suggest an association between IRS and Sertoli-Leydig cell tumours. Furthermore, we highlight the difficulties in detecting this ovarian tumour with sonography.
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| 4. |
- Eriksson, Lars, et al.
(författare)
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Miljöövervakning i Mälaren 2002
- 2003
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- På uppdrag av Mälarens vattenvårdsförbund har Institutionen för miljöanalys vid SLU iUppsala utfört provtagning, analys och utvärdering av vatten i Mälarens fjärdar under år2002.Föreliggande årsredogörelse beskriver huvuddragen av resultaten som dessutom bifogas i sinhelhet i tabellform. En fristående sammanfattning på 8 sidor har dessutom producerats ochdistribuerats.
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| 5. |
- Fernö, Mårten, et al.
(författare)
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Improved preparation technique of cervical carcinoma for flow cytometric DNA analysis with tissue disintegration in hydrochloric acid
- 1990
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Ingår i: In Vivo. - 0258-851X. ; 4:4, s. 225-229
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Tidskriftsartikel (refereegranskat)abstract
- An one-step procedure using a nuclear isolation medium containing propidium iodide has been found to be a suitable preparation technique for flow cytometric DNA analysis in breast cancer samples. In the case of cervical squamous carcinoma, a pretreatment with HCl seems to be a methodological improvement. One advantage with the HCl modification is that some "false" near-diploid cell populations are abolished. These "false" G0/G1 peaks may represent diploid nuclei with a different stainability for propidium iodide compared to normal diploid nuclei. The HCl treatment has, furthermore, the advantage of increasing the elution of nuclei (mean factor of 4.0), especially non-diploid nuclei from higher differentiated squamous carcinomas.
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| 6. |
- Fredstorp-Lidebring, M, et al.
(författare)
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Urokinase plasminogen activator and its inhibitor, PAI-1, in association with progression-free survival in early stage endometrial cancer
- 2001
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Ingår i: European Journal of Cancer. - 1879-0852. ; 37:18, s. 2339-2348
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Tidskriftsartikel (refereegranskat)abstract
- Components of the urokinase plasminogen activator (u-PA) system are involved in the metastatic process, and have accordingly been associated with clinical outcome in a variety of malignant tumours. We investigated the prognostic importance of u-PA and plasminogen activator inhibitor type 1 (PAI-1) in endometrial cancer, analysed with luminometric immunoassay (LIA) and enzyme-linked immunosorbent assay (ELISA), respectively. Two different cut-off levels were used: the median and the 80th percentile-the latter because of the low progression rate for patients with early stage (I-II) endometrial cancer. After a median follow-up time of 6.8 years, univariate analysis of patients with stage I-II disease (n=188) showed that high u-PA and high PAI-1 content was associated with a shorter progression-free survival (PFS), but at different cut-off levels, uPA at the median (P=0.003), and PAI-1 at the 80th percentile (P<0.001). Among the other factors, DNA ploidy status was most strongly correlated to PFS, followed by age (continuous), International Federation of Gynaecology and Obstetrics (FIGO) grade of differentiation, S-phase fraction and progesterone receptor (PgR) status. Bivariate analyses, including ploidy and one of the factors u-PA or PAI-1, showed that both add significant prognostic information. We conclude that u-PA and PAI-1 are promising prognostic factors in early stage endometrial cancer.
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| 7. |
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| 8. |
- Holmberg, Charlotte, 1972-
(författare)
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Så är det ju - den blir aldrig färdig! : En etnografisk studie om förskolepedagogers arbete med pedagogisk dokumentation
- 2015
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This dissertation is about educators work with pedagogical documentation in Swedish preschools. The purpose has been to investigate how pedagogical documentation is realized in everyday work in two preschools and examine how educators express themselves on purposes, opportunities and difficulties with this approach. This has been important to study since many preschools have chosen to work with pedagogic documentation to follow up and assure the quality of their work. Since 2010 pedagogical documentation is included in the preschools curriculum. The study was conducted at two different preschools where I followed a total of six educators in two preschool departments for two months in each department. Methods used to collect data were selected based on an ethnographic approach. Data collection consists of field notes, photographs, recorded interviews and documentations from preschools and their working plans. The analysis is based on a curriculum theory perspective, and especially important concepts have been transformation arena and realization arena. Furthermore, frame factor theory and the didactic questions what, how and why, have been used to analyze data. The results show that the documentation works where formulated in different ways depending on the frames and conditions. One preschool unit was at a municipal preschool without any special pedagogical approach. Here the pedagogical documentation was in a startup phase and was one of many tasks in the pedagogical work. The other preschool unit was in a Reggio Emilia inspired preschool who had worked with pedagogical documentation for several years. Here the work with pedagogical documentation was the driving force for developing the work. This ideological difference was reflected in what the educators documented, how they documented and how the documentations were used. Pedagogical documentation was partly be seen as a cumbersome way of working among the participants, whether they are beginners or experienced. It is in the nature of the work with pedagogical documentation that there are no obvious answers, which can lead to some uncertainty whether one is right or not. Other factors leading to frustration is that this approach is time-consuming and that the new technologies available require a lot of skills to be used. The study highlights that continuous training plays a crucial role as one of educator’s preconditions to succeed in the work with pedagogical documentation. In this study, one can also see how ethical aspects constantly makes themselves felt and need to be highlighted. The main impression is that the educators, despite the perceived difficulties, felt that it was stimulating and meaningful to work with pedagogical documentation and that they saw it as a tool for developing their work.
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| 9. |
- Hörnström, Einar, 1940-
(författare)
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Phytoplankton in Swedish oligotrophic lakes, affected by acidification, metals and liming
- 1999
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Anthropogenic airborne acidification has seriously affected thousands of lakes in Sweden, an alarming fact which was first noticed in the early 1970s, when pH had decreased to values below 5, especially in areas poor in limestone such as the Swedish west coast. This development was critical to phytoplankton, the diversity of which being markedly reduced, and where the groups Cyanophyceae, Bacillariophyceae and Conjugatophyceae were totally eliminated. A study of 500 lakes showed that this change was largely a result of low pH, since rather few species are acidophilic or acidotolerant. Secondary effects of acidification, such as phosphorus depletion and aluminium toxicity, however, were just as important, giving a combined stress upon the whole phytoplankton community, resulting in reduced species diversity. A large-scale remedial operation started in the middle of the 1970s, when the pH in many acidified lakes was increased to circumneutral values by addition of lime, in order to restore the original biotic communities. In several west coast lakes studied during 1973-1992, where the pH-values increased from 4.5-5.5 to 6.8-7.6, the treatments decreased the Al-levels and increased the concentrations of humic compounds and phosphorus in parallel with the decreased water transparency, compared with levels before liming. After a colonization period of 2-5 years, the species richness was fairly similar to that in unacidified lakes, and the species composition achieved indicated increased production. Results from long-term investigations in several limed lakes, and single analyses from other limed lakes, altogether making up a total of 63 limed lakes studied, were compared with the phytoplankton composition in 500 unlimed lakes. This showed that some species, common in unacidified lakes, were still missing several years after liming. Moreover, the original composition was not fully restored, results that could partly be explained by temporarily extreme pH-values and remaining ultraoligotrophic conditions. Regarding the consequences to the lake ecosystem, the absence of a few quantitatively unimportant species, however, does not seem to be a critical shortage.The toxicity of metals is one of the main stress factors depressing phytoplankton in lakes. The increased concentrations of inorganic and labile aluminium, caused toxic conditions that especially affected diatoms, chlorophyceans and desmids, and which were a main factor determining species composition in acidic lakes. Al-toxicity may occur temporarily also in neutral waters. Thus, laboratory tests showed that Monoraphidium griffithii was just as sensitive at pH 7 as at pH 4.8. In unacidified waters, increased concentrations of copper and zinc were recorded in connection with leakage from old mine tailings. During investigations in 41 and 32 lakes in 1992-1993 it was found that concentrations > 3-5 mg Cu l -1 and > 30 mg Zn l -1could be critical to some phytoplankton species. In the most contaminated lakes, with 1100-1500 mg Zn l -1 or 60-65 µg Cu l -1, the number of taxa was decreased by ca 50 %, but several species were frequent, indicating especially high tolerance. The lake waters were biologically characterized by algal assays. In the inorganic medium, the growth of Selenastrum capricornutum was strongly depressed at 1-2 mg Cu l -1, to be compared with 3-6 mg l -1 in lake waters with uncoloured substances, and 13-22 mg l -1 in waters with humic compounds. On the other hand, there was a positive relation between the number of diatom taxa and the manganese concentration.
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| 10. |
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