| 41. |
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| 42. |
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| 43. |
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| 44. |
- Gunnarsson, Ulrika, et al.
(författare)
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The Dark brown plumage color in chickens is caused by an 8.3 kb deletion upstream of SOX10.
- 2011
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Ingår i: Pigment cell & melanoma research. - 1755-148X .- 1755-1471.
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Tidskriftsartikel (refereegranskat)abstract
- The Dark brown mutation in chickens reduces expression of black eumelanin and enhances expression of red pheomelanin but only in certain parts of the plumage. Here we present genetic evidence that an 8.3 kb deletion upstream of the SOX10 transcription start site is the causal mutation underlying the Dark brown phenotype. The SOX10 transcription factor has a well-established role in melanocyte biology and is essential for melanocyte migration and survival. Previous studies have demonstrated that the mouse homolog of a highly conserved element within the deleted region is a SOX10 enhancer. The mechanism of action of this mutation remains to be established but one possible scenario is that the deletion leads to reduced SOX10 expression which in turn down-regulates expression of key enzymes in pigment synthesis such as tyrosinase. Lower tyrosinase activity leads to a shift towards a more pheomelanistic (reddish) plumage color, which is the characteristic feature of the Dark brown phenotype.
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| 45. |
- Jitpean, Supranee, et al.
(författare)
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Increased concentrations of Serum amyloid A in dogs with sepsis caused by pyometra
- 2014
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Ingår i: BMC Veterinary Research. - : Springer Science and Business Media LLC. - 1746-6148. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Background: Sepsis is a serious and potentially life-threatening condition and early diagnosis and appropriate treatment is crucial for survival. Pyometra is one of the most common diseases in intact female dogs. The disease often leads to sepsis (systemic inflammatory response syndrome, SIRS, caused by infection). Diagnostic markers for detecting sepsis are gaining increasing interest in veterinary medicine. Acute phase proteins (APPs) such as C-reactive protein (CRP) are useful for detecting systemic inflammation in dogs. Serum amyloid A (SAA) is another major APP in dogs that is not yet as widely used. Albumin is regarded as a negative APP and has earlier been evaluated for prediction of prognosis in septic dogs. The aim of the present study was to determine SAA, CRP and albumin concentrations in dogs with sepsis and pyometra and to evaluate whether these inflammatory markers are associated with length of postoperative hospitalization.Results: Thirty-one surgically treated bitches with pyometra were included, whereof 23 septic (SIRS-positive) and eight non-septic (SIRS-negative). Albumin concentrations were analyzed by routine automated methods. SAA and CRP analyses were performed with previously validated commercially available assays (ELISA and immunoturbidimetric). Mean (+/- SE) serum concentrations of SAA were significantly higher in septic (130.8 +/- 8.0 mg/L) compared to non-septic bitches (88.5 +/- 12.5 mg/L). Using a cut-off value for SAA of 109.07 mg/L (n = 31 bitches), the sensitivity and specificity for detecting sepsis was 74% and 50%, respectively. Serum albumin concentrations were not significantly different in septic compared to non-septic bitches (mean +/- SE, 25 +/- 1 g/L and 26 +/- 1 g/L, respectively). CRP concentrations were also not significantly different in septic (mean +/- SE 225.6 +/- 16.0 mg/L) compared to non-septic bitches (mean +/- SE, 176.0 +/- 27.1 mg/L). None of these inflammatory markers were associated with the outcome as measured by length of hospitalization.Conclusions: SAA concentrations were increased in dogs with sepsis induced by pyometra and may be useful as an adjunctive diagnostic marker for sepsis. To evaluate the full potential of SAA as a marker for sepsis also in other diseases, further studies are warranted.
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| 46. |
- Ley, Charles, et al.
(författare)
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Osteochondral lesions in distal tarsal joints of Icelandic horses reveal strong associations between hyaline and calcified cartilage abnormalities.
- 2014
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Ingår i: eCells and Materials Journal. - 1473-2262. ; 27, s. 213-236
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Tidskriftsartikel (refereegranskat)abstract
- Osteochondral lesions in the joints of the distal tarsal region of young Icelandic horses provide a natural model for the early stages of osteoarthritis (OA) in low-motion joints. We describe and characterise mineralised and non-mineralised osteochondral lesions in left distal tarsal region joint specimens from twenty-two 30 ±1 month-old Icelandic horses. Combinations of confocal scanning light microscopy, backscattered electron scanning electron microscopy (including, importantly, iodine staining) and three-dimensional microcomputed tomography were used on specimens obtained with guidance from clinical imaging. Lesion-types were described and classified into groups according to morphological features. Their locations in the hyaline articular cartilage (HAC), articular calcified cartilage (ACC), subchondral bone (SCB) and the joint margin tissues were identified and their frequency in the joints recorded. Associations and correlations between lesion-types were investigated for centrodistal joints only. In centrodistal joints the lesion-types HAC chondrocyte loss, HAC fibrillation, HAC central chondrocyte clusters, ACC arrest and ACC advance had significant associations and strong correlations. These lesion-types had moderate to high frequency in centrodistal joints but low frequencies in tarsometatarsal and talocalcaneal-centroquartal joints. Joint margin lesion-types had no significant associations with other lesion-types in the centrodistal joints but high frequency in both the centrodistal and tarsometatarsal joints. The frequency of SCB lesion-types in all joints was low. Hypermineralised infill phase lesion-types were detected. Our results emphasise close associations between HAC and ACC lesions in equine centrodistal joints and the importance of ACC lesions in the development of OA in low-motion compression-loaded equine joints.
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| 47. |
- Melin, Petter, et al.
(författare)
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Formulation and stabilisation of the biocontrol yeast Pichia anomala
- 2011
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Ingår i: Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology. - Dordrecht, Netherlands : Springer Netherlands. - 0003-6072 .- 1572-9699. ; 99:1, s. 107-112
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Tidskriftsartikel (refereegranskat)abstract
- The yeast Pichia anomala has antifungal activities and its potential in biocontrol and biopreservation has previously been demonstrated. To practically use an organism in such applications on a larger scale the microbe has to be formulated and stabilised. In this review we give an overview of our experience of formulating and stabilising P. anomala strain J121 in a wider perspective. The stabilisation techniques we have evaluated were liquid formulations, fluidised bed drying, lyophilisation (freeze-drying) and vacuum drying. With all methods tested it was possible to obtain yeast cells with shelf lives of at least a few months and in all cases the biocontrol activity was retained. Fluidised bed drying was dependent on the addition of cottonseed flour as a carrier during the drying process. In liquid formulations a sugar, preferentially trehalose, was a required additive. These two kinds of microbial stabilisation are easily performed and relatively inexpensive but in order to keep the cells viable the biomaterial has to be stored at cool temperatures. However, there is room for optimization, such as improving the growth conditions, or include preconditioning steps to enable the cells to produce more compatible solutes necessary to survive formulation, desiccation and storage. In contrast, lyophilisation and vacuum drying require a lot of energy and are thus expensive. On the other hand, the dried cells were mostly intact after one year of storage at 30A degrees C. Inevitably, the choice of formulation and stabilisation techniques will be dependent also on the intended use.
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| 48. |
- Norrgran, Jessica, et al.
(författare)
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Decabromobiphenyl, Polybrominated Diphenyl Ethers, and Brominated Phenolic Compounds in Serum of Cats Diagnosed With the Endocrine Disease Feline Hyperthyroidism
- 2012
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Ingår i: Archives of Environmental Contamination and Toxicology. - : Springer Science and Business Media LLC. - 0090-4341 .- 1432-0703. ; 63:1, s. 161-168
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Tidskriftsartikel (refereegranskat)abstract
- The incidence of cats being diagnosed with feline hyperthyroidism (FH) has increased greatly since it was first described in 1979. The cause of FH has not been established. Hypothetically, there is a link between increasing FH and exposure to brominated flame retardants. Much greater polybrominated diphenyl ethers (PBDE) concentrations have been reported in cat serum compared with human serum, likely due to cat licking behaviour. This study aimed to extend the present identification of brominated compounds in cat serum, with a focus on hydroxylated metabolites of PBDE, to improve the understanding of feline metabolism of PBDEs. A pooled serum sample from 30 Swedish pet cats with FH was analysed, and brominated species were identified. The results showed exposure to the discontinued flame retardant decabromobiphenyl (BB-209) and technical penta- and octa-BDEs. Altogether 12 PBDE congeners were identified along with 2'-MeO-BDE68. Furthermore, 2,4-dibromophenol, 2,4,6-, 2,4,5- and 2,3,4-tribromophenol plus 2'-OH-BDE68, 6-OH-BDE47, 5-OH-BDE47, 4'-OH-BDE49 were identified. 2,4,6-tribromophenol and 6-OH-BDE47 were the most prominent species in cat serum. Considering that these are natural products, it can be concluded that metabolism of PBDEs to OH-PBDEs is not a major route of PBDE elimination in cats. It is notable that BB-209, 6-OH-BDE47, and 2,4,6-tribromophenol all suggested that endocrine-disrupting chemicals were present in high concentrations in cat serum.
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| 49. |
- Elzinga, Aant, 1937
(författare)
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Drömmar om en elefant. Några ämneshistoriska anteckningar
- 2009
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Ingår i: Vi vet något. Festskrift till Jan Bärmark, Margareta Hallberg (red.). - Göteborg : University of Gothenburg. - 9789197623919 ; , s. 73-93
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Bakgrunden till ämnet vetenskapsteorins införande vid Göteborgs universitet och dess utveckling såsom en del av "forskning om forskning" och senare koppling till vetenskapsstudier (STS) tas upp på basis av en genomgång av skrifter och dokument fram till 1995. Kapitlet innehåller en tabell med 25 nyckelhändelser mellan 1975-1995. Analogin med "drömman om en elefant" anspelar på titeln på en bok red. av J. Bärmark 1984, Forskning om forskning, eller konsten att beskriva en elefant. Framställningen i kapitlet inkluderar ett avsnitt om det antika indiska ämnet "Elefantologi" och antyder att "Forskning om forskning" blev en "vit elefant".
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| 50. |
- Hansson, Helena, et al.
(författare)
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Measuring farmers' attitudes to animal welfare and health
- 2012
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Ingår i: British Food Journal. - : Emerald. - 0007-070X .- 1758-4108. ; 114, s. 840-852
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study was to develop a behavioural framework for developing a scale to measure farmers' attitudes to animal welfare and health and to take an explorative approach to initiating development of such a scale.Design/methodology/approachA literature review was used to develop the behavioural framework. Exploratory factor analysis was then used to initiate development of a measurement scale, based on a sample of 108 Swedish livestock farmers.FindingsBased on the framework developed, the authors' data suggest unidimensionality of farmers' attitudes to animal welfare and health; and that farmers perceive animal welfare as being about animal health and comfort in particular.Research limitations/implicationsFurther research should be devoted to this area to develop a more final measurement scale. This could be done by re‐evaluating the scale obtained in this paper in both exploratory and confirmatory factor analysis settings. The behavioural framework proposed here provides a basis for such scale development and a more rigid framework for evaluating and comparing farmers' attitudes to animal welfare.Practical implicationsThe behavioural framework and scale development initiated in this paper can be used by policymakers and organizations responsible for quality assurance schemes to develop policy measures and education programmes to re‐train farmers' behaviour into a system that supports higher animal welfare and health standards.Originality/valueThe originality of this paper is that it develops and uses a behavioural framework based on psychological and psychometric theory to initiate development of a scale to measure farmers' attitudes to animal welfare and health.
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