| 3561. |
- Einarsdottir, Sigrun, et al.
(author)
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Vaccination against tick-borne encephalitis (TBE) after autologous and allogeneic stem cell transplantation
- 2021
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In: Vaccine. - : Elsevier BV. - 0264-410X .- 1873-2518. ; 39:7, s. 1035-1038
-
Journal article (peer-reviewed)abstract
- Introduction: Our aim was to assess response and side effects of 4 doses of TBE vaccine to patients (pts) after allo- and autologous stem cell transplantation (SCT). PATIENTS: Included were 104 pts with leukaemia, myeloma and lymphoma, median age 61 yrs. METHODS: Vaccine (FSME-Immun (R)) was given at 9, 10, 12, and 21 months post-transplant. Serum samples were obtained before and after vaccinations. Healthy controls (n = 27) received 3 vaccinations. Assessments of TBE specific IgG antibodies were performed by Enzygnost anti-TBE ELISA test (Siemens, Sweden). Results: Antibody levels (>12 U/mL; "seropositivity") were seen in 77% and 80% of pts after allo- and autoSCT; IgG levels; 89 vs 94 U/mL. Ongoing chronic GvHD and immunosuppression (n = 29) was associated with sero-negativity in the last sample (p = 0.007). All controls (n = 27) developed protective antibody levels. Conclusions: TBE vaccination was safe, and 4 doses starting 9 months post-SCT, induced seropositivity in a vast majority of pts. (C) 2021 Elsevier Ltd. All rights reserved.
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| 3562. |
- Eisenacher, Martin, et al.
(author)
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Proteomics Data Collection - 2nd ProDaC Workshop 5 October 2007, Seoul, Korea
- 2008
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In: Proteomics. - : Wiley. - 1615-9861 .- 1615-9853. ; 8:7, s. 1326-1330
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Journal article (peer-reviewed)abstract
- Proteomics Data Collection (ProDaC) is an EU-funded Coordination Action within the 6th framework programme. It aims to simplify the publication, dissemination and utilization of proteomics data by establishing standards that will support broad data collection from the research community. As a part of ProDaC, regular workshops are organized on a half-yearly basis to enable communication and discussion of the involved partners and to report on project progress. After the kick-off meeting (October 2006) in Long Beach, CA, USA and the 1st workshop in Lyon, France (April 2007), the 2nd ProDaC workshop took place at the COEX InterContinental Hotel in Seoul, Korea, on 5th October 2007, shortly before the HUPO World Congress. The progress achieved within the first year was presented by the leaders of the work packages. Additionally, a Journal's representative talked about his experiences and future plans concerning Proteomics standards; and two further external speakers presented their research related to data handling and Proteomics repositories.
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| 3563. |
- Eisenacher, Martin, et al.
(author)
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Proteomics data collection--4th ProDaC workshop 15 August 2008, Amsterdam, The Netherlands
- 2009
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In: Proteomics. - : Wiley. - 1615-9861 .- 1615-9853. ; 9:2, s. 218-222
-
Journal article (peer-reviewed)abstract
- ProDaC (Proteomics Data Collection), a Coordination Action within the 6th EU framework programme, was created to support the collection, distribution and public availability of data from proteomics experiments. Within the consortium standards are created and maintained enabling an extensive data collection within the proteomics community. Important elements of ProDaC are workshops held twice a year to allow communication between the ProDaC partners and to report the ongoing progress. The most recent assembly was the 4th ProDaC workshop on August 15th, 2008, in Amsterdam, The Netherlands. It took place directly before the 7th HUPO Annual World Congress (Human Proteome Organisation). Work package coordinators and partners presented the progress achieved since the last meeting. Additionally, an EU official presented funding opportunities for proteomics in the next EU framework programme and five external speakers presented talks about their work in relation to ProDaC.
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| 3564. |
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| 3565. |
- Ek, Sara, et al.
(author)
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Gene expression profiling of mantle cell lymphonas
- 2003
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In: Immunology Letters (Abstracts of the 15th European Immunology Congress (EFIS 2003)). - 0165-2478 .- 1879-0542. ; 87:1-3, s. 01-24
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Conference paper (other academic/artistic)
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| 3566. |
- Ek, Sara, et al.
(author)
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Parallel gene expression profiling of mantle cell lymphoma - How do we transform 'omics data into clinical practice
- 2007
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In: Current Genomics. - : Bentham Science Publishers Ltd.. - 1389-2029. ; 8:3, s. 171-179
-
Journal article (peer-reviewed)abstract
- DNA microarray technology has been a valuable tool to provide a global view of the changes in gene expression that characterize different types of B cell lymphomas, both in relation to clinical parameters but also in comparison with the non-malignant counterparts. The number of transcripts that can be analyzed on an array has dramatically increased, and now most commercially available arrays cover the whole genome, enabling overall analysis of the transcriptome. The backside of collecting this massive amount of information is that even after strict data filtering, it is impossible to do follow-up studies on all findings. Down-stream analysis is time-consuming and when performing confirmatory experiments on the protein level, the experiments are in most cases restricted to proteins recognized by commercially available reagents. Furthermore, since gene expression data is a comparative method not only are the experimental set-up but also the characteristics of both the sample and reference crucial for our ability to answer the questions posed. Thus, initial care must be taken in the design of the experiment and the preparation of the samples. The aim of this review is to discuss the progress in mantle cell lymphoma research enabled by gene expression analysis and to pinpoint the difficulties in making efficient use of the generated data to provide a fast and accurate clinical diagnosis, efficient stratification of patients into disease sub-groups and improved therapy.
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| 3567. |
- Ek, Sara
(author)
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Transcriptional analysis of mantle cell lymphoma - a global search for cellular origin and therapeutic targets
- 2004
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Doctoral thesis (other academic/artistic)abstract
- This thesis is based upon four original papers describing the characterisation of mantle cell lymphoma (MCL) tumours and cell lines in comparison with different populations of normal B cells. Gene expression profiling, which were used in the studies, has during recent years developed as an important tool in characterisation of different types of malignancies. In this thesis, the technique is used to identify gene signatures in addition to providing information about the mRNA expression of more than 10 000 genes in the samples analysed. We found indications of an antigen-activated origin of MCL, in contrast to the current dogma. De-regulation of apoptosis and proliferation-associated pathways were further identified and possibly therapeutic targets were recognised. In an attempt to define the differences between high and low proliferative MCL, a proliferation-associated signature was established. The genes or gene-products constituting the signature are potential targets for therapy, as the genes are associated with proliferation, which is related to decreased survival time. Furthermore, MCL cell lines were studied to determine their usefulness as in vitro models for MCL. Hierarchical clustering, using a MCL-specific gene list, confirmed that the cell lines had preserved the expression of MCL-associated genes, as they were readily separate from other lymphoma cell lines, primary follicular lymphoma samples and normal B cells. However, one of the MCL-derived cell lines showed less correlation to the primary MCL tumours than the other cell lines. The same cell line carried a mutated VH gene, in contrast to the other MCL cell lines, and may be more suitable as in vitro model for MCL with mutated than unmutated VH genes.
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| 3568. |
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| 3569. |
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| 3570. |
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