| 81. |
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| 82. |
- Linhult, M., et al.
(författare)
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Affinity ligands for industrial protein purification
- 2005
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Ingår i: Protein peptide letters. - : Bentham Science Publishers Ltd.. - 0929-8665 .- 1875-5305. ; 12:4, s. 305-310
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Forskningsöversikt (refereegranskat)abstract
- Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.
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| 83. |
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| 84. |
- Nordquist, J, et al.
(författare)
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Does ultrasound influence experimentally induced thrombus formation in the central artery of the rabbit ear?
- 2000
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Ingår i: Journal of Thrombosis and Thrombolysis. - 1573-742X. ; 9:3, s. 243-249
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Thrombosis is one of the most important causes of morbidity in the medical field. Several independent in vitro studies have shown that the fibrinolytic process may be enhanced by ultrasound, but the effect of ultrasound on thrombus formation in vivo is unexplored. The present study was designed to investigate this matter. METHODS: In a blind randomized study, standardized arteriotomies and intimectomies were performed on the central arteries of the ears of 25 rabbits. The rabbits were allocated to two groups, an untreated control group and a group treated with ultrasound (10 pulses of frequency 1 MHz and intensity 1 W/cm(2) per millisecond giving an averaged intensity of 0.01 W/cm(2)). Immediately after reperfusion, patency was confirmed by a manual empty/refill test, after which blood-flow was monitored using ultrasonic flow-probes twice a minute for two hours. At two hours, patency was rechecked. RESULTS: All vessels were patent at reperfusion, but only seven vessels (three control, four treated) were patent when flow-rate measurements started. At 2 h, patency-frequencies were 12/23 in the control group and 11/22 in the treated group. Flow-rate curves in patent vessels in both groups were similar. Microscopic investigation at one week showed no difference in thrombus accumulation. CONCLUSIONS: Ultrasound with the above characteristics does not significantly improve patency in vivo.
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| 85. |
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| 86. |
- Palm-Espling, Maria, et al.
(författare)
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Interaction between the anticancer drug cisplatin and the copper chaperone Atox1 in human melanoma cells
- 2014
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Ingår i: Protein Peptide Letters. - : Bentham Science Publishers Ltd.. - 0929-8665 .- 1875-5305. ; 21:1, s. 63-68
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Tidskriftsartikel (refereegranskat)abstract
- Cisplatin (CisPt) is one of the most common anticancer drugs used against many severe forms of cancers. However, treatment with this drug causes many side effects and often, it results in the development of cell resistance. A majority of side effects as well as cell resistance are thought to develop due to CisPt interactions with proteins prior to reaching the nucleus and the DNA target. The copper (Cu) transport proteins Ctr1 and ATP7A/B have been implicated in cellular resistance of CisPt, possibly exporting the drug out of the cell. Recent in vitro work demonstrated that CisPt also interacts with the cytoplasmic Cu-chaperone Atox1, binding in or near the Cu-binding site, without expulsion of bound Cu. Whereas Ctr1 and ATP7B interactions with CisPt have been shown in vivo or ex vivo, there is no such information for Atox1-CisPt interactions. To address this, we developed a method to probe if CisPt interacts with Atox1 in human melanoma cells. Atox1-specific antibodies were linked to magnetic beads and used to immune-precipitate Atox1 from melanoma cells that had been pre-exposed to CisPt. Analysis of extracted Atox1 with inductively coupled plasma mass spectrometry demonstrated the presence of Pt in the protein fraction. Thus, CisPt-exposed human melanoma cells contain Atox1 molecules that bind some derivative of CisPt. This study gives the first indication for the intracellular presence of Atox1-CisPt complexes ex vivo. © 2014 Bentham Science Publishers.
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| 87. |
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| 88. |
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| 89. |
- Tanhaian, Abbas, et al.
(författare)
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In Silico and In Vitro Investigation of a Likely Pathway for Anti-Cancerous Effect of Thrombocidin-1 as a Novel Anticancer Peptide
- 2020
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Ingår i: Protein peptide letters. - : Bentham Science Publishers Ltd. - 0929-8665 .- 1875-5305. ; 27:8, s. 751-762
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Tidskriftsartikel (refereegranskat)abstract
- Background: Antimicrobial and antifungal activities of Thrombocidin-1 (TC-1) is shown previously, however, the anti-cancerous feature of this peptide is still uncovered. Objective: The objective is to evaluate anti-cancerous feature of recombinant TC-1. Methods: In this study, based on the significant similarity of rTC-1 and IL-8 in case of coding sequence, tertiary structure, and also docking and molecular dynamic simulation (MD) results with CXCR1, a receptor which has positive correlation with different cancers, a likely pathway for anti-cancerous effect of rTC-1 was proposed. In addition, the coding sequence of TC-1+6 x histidine (rTC-1) was inserted into the pET22b(+) vector and cloned and expressed by E. coli BL21 and finally purified through nickel affinity column. Afterward, the retrieved rTC-1 was used in MTT assay against mouse colon adenocarcinoma, hepatocellular carcinoma, chondrosarcoma, mouse melanoma, and breast adenocarcinoma cell lines to investigate its probable anticancer application. Results: Docking and MD simulation results showed that rTC-1 and IL-8 share almost the same residues in the interaction with CXCR1 receptor. Besides, the stability of the rTC-l_CXCR1(1-)(38) complex was shown during 100ns MD simulation. In addition, the successful expression and purification of rTC-1 depict an 8kD peptide. The IC50 results of MTT assay revealed that rTC-1 has cytotoxic effect on C26-A and SW1353 cancerous cell lines. Conclusion: Therefore, apart from probable anti-cancerous effect of rTC-1 on C26-A and SW1353 cell lines, this peptide may be able to mimic the anti-cancerous pathway of IL-8.
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| 90. |
- Wade, D, et al.
(författare)
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Structural analysis of Efb, a fibrinogen binding protein of Staphylococcus aureus
- 1998
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Ingår i: Protein peptide letters. - 0929-8665 .- 1875-5305. ; 5:4, s. 199-206
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Tidskriftsartikel (refereegranskat)abstract
- Staphylococcus aureus produces and secretes a small, basic protein, designated Efb, that binds to fibrinogen and seems to be required for virulence of the organism. A 3D model of Efb was developed, and it predicts that the C-terminal half of the protein contains substantial alpha-helical content. CD analysis of Efb yielded a value of 40-41% alpha-helix. Calcium and zinc both influence the interactions between Efb and fibrinogen, and an analysis of the amino acid sequence of Efb revealed the presence of consensus sequences for the binding of both metals.
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