| 391. |
- Mojumdar, Enamul Haque, et al.
(författare)
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Probing skin barrier recovery on molecular level following acute wounds : An in vivo/ex vivo study on pigs
- 2021
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Ingår i: Biomedicines. - : MDPI AG. - 2227-9059. ; 9:4
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Tidskriftsartikel (refereegranskat)abstract
- Proper skin barrier function is paramount for our survival, and, suffering injury, there is an acute need to restore the lost barrier and prevent development of a chronic wound. We hypothesize that rapid wound closure is more important than immediate perfection of the barrier, whereas specific treatment may facilitate perfection. The aim of the current project was therefore to evaluate the quality of restored tissue down to the molecular level. We used Göttingen minipigs with a multi-technique approach correlating wound healing progression in vivo over three weeks, monitored by classical methods (e.g., histology, trans-epidermal water loss (TEWL), pH) and subsequent physicochemical characterization of barrier recovery (i.e., small and wide-angle X-ray diffraction (SWAXD), polarization transfer solid-state NMR (PTssNMR), dynamic vapor sorption (DVS), Fourier transform infrared (FTIR)), providing a unique insight into molecular aspects of healing. We conclude that although acute wounds sealed within two weeks as expected, molecular investigation of stratum corneum (SC) revealed a poorly developed keratin organization and deviations in lipid lamellae formation. A higher lipid fluidity was also observed in regenerated tissue. This may have been due to incomplete lipid conversion during barrier recovery as glycosphingolipids, normally not present in SC, were indicated by infrared FTIR spectroscopy. Evidently, a molecular approach to skin barrier recovery could be a valuable tool in future development of products targeting wound healing.
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| 392. |
- Mondlane, Avelino, et al.
(författare)
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E-Governance and Natural Hazards in Mozambique : A Challenge for Backasting Method Used for Flood Risk Management Strategies
- 2014
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Ingår i: Emerging Issues And Prospects In African E-Government. - : IGI Global. - 9781466662964 - 9781466662971 - 1466662964 ; , s. 253-268
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Strategic planning is a decisive process toward sustainable development for any organization. Mozambique has developed many tools toward good governance, among which Poverty Alleviation Strategy Paper (PARPA) is an umbrella. PARPA includes different key decisive segments of society, particularly the Information and Communication Technologies (ICTs) as the pool for development. This chapter investigates to what extent e-Governance, particularly the development of strategies based on ICTs, can contribute to minimize the impact of floods at local governments by addressing best practice and decision-making process. The authors address backcasting methodology as an approach to consider in a participatory strategic planning for long-term decision-making processes. They use Chókwe District as a showcase where e-governance has an impact in mitigating and preventing the impact of floods.
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| 393. |
- Mondlane, Avelino I., et al.
(författare)
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Insurance as Strategy for Flood Risk Management at Limpopo River Basin – A decision making Process under Uncertainty
- 2013
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Ingår i: International Journal of Computers & Technology. - : CIRWOLRD. - 2277-3061. ; 10:8, s. 1862-1877
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Tidskriftsartikel (refereegranskat)abstract
- The Limpopo River Basin, one of the nine international rivers crossing Mozambique, historically has shown to be cyclically flooded, undermining the economic and social development of its four riparian countries. Local and external added efforts are always in place when floods occur. Nowadays there are recommended ex-ante instruments to prevent floods and one of the most applicable instruments worldwide is insurance. Most of the inhabitants, even governments, affected by Limpopo River Basin are poor, therefore our concern regards the viability to apply insurance as a strategy for flood risk management. Moreover our research investigates to what extent the application of insurance, within two identified communities as case study, might create an added value in the process of decision making on flood risk management for Limpopo River Basin.
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| 394. |
- Mondlane, Avelino, et al.
(författare)
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ICT for Flood Risk Management Strategies : a GIS-based MCDA (M) Approach
- 2013
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Ingår i: 2013 IST-Africa Conference & Exhibition <em></em>. - : IEEE Computer Society. - 9781905824380 ; , s. 1-9
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Konferensbidrag (refereegranskat)abstract
- This paper discusses the application of Analytical Hierarchical (AHP) on Geographic Information System (GIS) based Multcriteria Decision Analysis (Making) MCDA (M) for flood risk management strategies at the Limpopo River Basin in Mozambique. An analytical process is carried out based on GIS Decision Support System (DSS) taking into account the role of ICT and interest of both the decision makers and different stakeholders namely the householders and economic agents, within the basin. The study aims to provide the different stakeholders and decision makers with a decision tool and it focuses on the Mozambican section Limpopo River Basin as part of the national strategies to mitigate the exposure to both floods and droughts that cyclically affect the region.
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| 395. |
- Mondlane, Avelino, et al.
(författare)
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ICT for Social Networking in Flood Risk and Knowledge Management Strategies - an MCDA approach
- 2013
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Ingår i: International Journal of Computer and Information Science and Engineering. - 1307-4164. ; 7:10, s. 330-336
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Tidskriftsartikel (refereegranskat)abstract
- This paper discusses the role and importance of Information and Communication Technologies (ICT) and social Networking (SN) in the process of decision making for Flood Risk and Knowledge Management Strategies. We use Mozambique Red Cross (CVM) as the case study and further more we address scenarios for flood risk management strategies, using earlier warning and social networking and we argue that a sustainable desirable stage of life can be achieved by developing scenario strategic planning based on backcasting.
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| 396. |
- Mondlane, Avelino, et al.
(författare)
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Vulnerability, Human Behaviour, Hazards and Expected Utility in the Context of Risk Management : “The case of Limpopo River Basin in Mozambique”
- 2013
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Ingår i: International Journal of Computer Science and Network. - 2277-5420. ; 2:6, s. 1-11
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Tidskriftsartikel (refereegranskat)abstract
- In this paper we use four main dimensions: Vulnerability, Human Behaviour, Hazards and Expected Utility to analyze their impact in scenario planning when cross-matched with Human Development Adjusted, Gender Inequality and Multidimensional Poverty Indexes within flood risk management strategies. We argue that the four dimensions are among the central factors behind the poor quality of life. Hence, we propose a backcasting method for a scenario planning and based on sustainable principles at long run to provide a desired and better quality of life as a contribution by the human beings in reducing vulnerability to risk and exposure to hazards. In doing so, we address best practices toward utility improvement and behaviour paradigm shift as a novel approach for participatory strategic thinking in the Multicriteria Decision Analysis for integrating flood risk management strategies related to Limpopo River Basin.
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| 397. |
- Morén, Björn, et al.
(författare)
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EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes
- 2019
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Ingår i: Molecular Biology of the Cell. - : AMER SOC CELL BIOLOGY. - 1059-1524 .- 1939-4586. ; 30:10, s. 1147-1159
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Tidskriftsartikel (refereegranskat)abstract
- Adipocytes play a central role in energy balance, and dysfunctional adipose tissue severely affects systemic energy homeostasis. The ATPase EH domain-containing 2 (EHD2) has previously been shown to regulate caveolae, plasma membrane-specific domains that are involved in lipid uptake and signal transduction. Here, we investigated the role of EHD2 in adipocyte function. We demonstrate that EHD2 protein expression is highly up-regulated at the onset of triglyceride accumulation during adipocyte differentiation. Small interfering RNA-mediated EHD2 silencing affected the differentiation process and impaired insulin sensitivity, lipid storage capacity, and lipolysis. Fluorescence imaging revealed localization of EHD2 to caveolae, close to cell surface-associated lipid droplets in primary human adipocytes. These lipid droplets stained positive for glycerol transporter aquaporin 7 and phosphorylated perilipin-1 following adrenergic stimulation. Further, EHD2 overexpression in human adipocytes increased the lipolytic signaling and suppressed the activity of transcription factor PPAR.. Overall, these data suggest that EHD2 plays a key role for adipocyte function.
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| 398. |
- Müller, Thomas R., et al.
(författare)
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Additive effects of booster mRNA vaccination and SARS-CoV-2 Omicron infection on T cell immunity across immunocompromised states
- 2023
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Ingår i: Science Translational Medicine. - 1946-6234 .- 1946-6242. ; 15:704, s. eadg9452-
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Tidskriftsartikel (refereegranskat)abstract
- Suboptimal immunity to SARS-CoV-2 mRNA vaccination has frequently been observed in individuals with various immunodeficiencies. Given the increased antibody evasion properties of emerging SARS-CoV-2 subvariants, it is necessary to assess whether other components of adaptive immunity generate resilient and protective responses against infection. We assessed T cell responses in 279 individuals, covering five different immunodeficiencies and healthy controls, before and after booster mRNA vaccination, as well as after Omicron infection in a subset of patients. We observed robust and persistent Omicron-reactive T cell responses that increased markedly upon booster vaccination and correlated directly with antibody titers across all patient groups. Poor vaccination responsiveness in immunocompromised or elderly individuals was effectively counteracted by the administration of additional vaccine doses. Functionally, Omicron-reactive T cell responses exhibited a pronounced cytotoxic profile and signs of longevity, characterized by CD45RA+ effector memory subpopulations with stem cell-like properties and increased proliferative capacity. Regardless of underlying immunodeficiency, booster-vaccinated and Omicron-infected individuals appeared protected against severe disease and exhibited enhanced and diversified T cell responses against conserved and Omicron-specific epitopes. Our findings indicate that T cells retain the ability to generate highly functional responses against newly emerging variants, even after repeated antigen exposure and a robust immunological imprint from ancestral SARS-CoV-2 mRNA vaccination.
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| 399. |
- Müller, Thomas R., et al.
(författare)
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Memory T cells effectively recognize the SARS-CoV-2 hypermutated BA.2.86 variant
- 2024
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Ingår i: Cell Host and Microbe. - : Elsevier. - 1931-3128 .- 1934-6069. ; 32:2, s. 156-161.e3
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Tidskriftsartikel (refereegranskat)abstract
- T cells are critical in mediating the early control of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) breakthrough infection. However, it remains unknown whether memory T cells can effectively cross-recognize new SARS-CoV-2 variants with a broad array of mutations, such as the emergent hypermutated BA.2.86 variant. Here, we report in two separate cohorts, including healthy controls and individuals with chronic lymphocytic leukemia, that SARS-CoV-2 spike-specific CD4+ and CD8+ T cells induced by prior infection or vaccination demonstrate resilient immune recognition of BA.2.86. In both cohorts, we found largely preserved SARS-CoV-2 spike-specific CD4+ and CD8+ T cell magnitudes against mutated spike epitopes of BA.2.86. Functional analysis confirmed that both cytokine expression and proliferative capacity of SARS-CoV-2 spike-specific T cells to BA.2.86-mutated spike epitopes are similarly sustained. In summary, our findings indicate that memory CD4+ and CD8+ T cells continue to provide cell-mediated immune recognition to highly mutated emerging variants such as BA.2.86.
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| 400. |
- Nahi, Hareth, et al.
(författare)
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Incidence, characteristics, and outcome of solitary plasmacytoma and plasma cell leukemia. Population-based data from the Swedish Myeloma Register
- 2017
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Ingår i: European Journal of Haematology. - : Wiley. - 0902-4441 .- 1600-0609. ; 99:3, s. 216-222
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Tidskriftsartikel (refereegranskat)abstract
- Solitary plasmacytoma (SP) and plasma cell leukemia (PCL) are uncommon (3-6%) types of plasma cell disease. The risk of progression to symptomatic multiple myeloma (MM) is probably important for the outcome of SP. PCL is rare and has a dismal outcome. In this study, we report on incidence and survival in PCL/SP, and progression to MM in SP, using the prospective observational Swedish Multiple Myeloma Register designed to document all newly diagnosed plasma cell diseases in Sweden since 2008. Both solitary bone plasmacytoma (SBP) (n=124) and extramedullary plasmacytoma (EMP) (n=67) have better overall survival (OS) than MM (n=3549). Progression to MM was higher in SBP than in EMP (35% and 7% at 2 years, respectively), but this did not translate into better survival in EMP. In spite of treatment developments, the OS of primary PCL is still dismal (median of 11 months, 0% at 5 years). Hence, there is a great need for diagnostic and treatment guidelines as well as prospective studies addressing the role for alternative treatment options, such as allogeneic stem cell transplantation and monoclonal antibodies in the treatment of PCL.
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