| 3161. |
- Strömberg, Sara, et al.
(författare)
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A high-throughput strategy for protein profiling in cell microarrays using automated image analysis
- 2007
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Ingår i: Proteomics. - : Wiley. - 1615-9853 .- 1615-9861. ; 7:13, s. 2142-2150
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Tidskriftsartikel (refereegranskat)abstract
- Advances in antibody production render a growing supply of affinity reagents for immunohistochemistry (IHC), and tissue microarray (TMA) technologies facilitate simultaneous analysis of protein expression in a multitude of tissues. However, collecting validated IHC data remains a bottleneck problem, as the standard method is manual microscopical analysis. Here we present a high-throughput strategy combining IHC on a recently developed cell microarray with a novel, automated image-analysis application (TMAx). The software was evaluated on 200 digital images of IHC-stained cell spots, by comparing TMAx annotation with manual annotation performed by seven human experts. A high concordance between automated and manual annotation of staining intensity and fraction of IHC-positive cells was found. in a limited study, we also investigated the possibility to assess the correlation between mRNA and protein levels, by using TMAx output results for relative protein quantification and quantitative real-time PCR for the quantification of corresponding transcript levels. In conclusion, automated analysis of immunohistochemically stained in vitro-cultured cells in a microarray format can be used for high-throughput protein profiling, and extraction of RNA from the same cell lines provides a basis for comparing transcription and protein expression on a global scale.
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| 3162. |
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| 3163. |
- Stubbe, Ingo, et al.
(författare)
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High-density lipoprotein concentrations increase after stopping smoking
- 1982
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Ingår i: British Medical Journal (Clinical Research Edition). - 0267-0623. ; 284:6328, s. 1511-1513
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Tidskriftsartikel (refereegranskat)abstract
- Concentrations of plasma lipoproteins in 10 men who were habitual smokers were monitored for six weeks after they stopped smoking and related to changes in diet and body weight. The energy intake increased by 10% (p less than 0.05) owing to a higher consumption of carbohydrates and fat, and body weight increased by 2% (p less than 0.01). Plasma triglyceride, cholesterol, and low-density lipoprotein cholesterol concentrations did not change significantly. The most prominent finding was a rapid and pronounced increased in high-density lipoprotein concentrations. From comparatively low values (mean 0.82 mmol/1) they rose by 29% (p less than 0.01) within two weeks and remained at this value throughout the observation period. In three subjects who resumed smoking after the end of the study they again fell to initial values six weeks later. The initial increase in concentration could be accounted for mainly by an increase in the esterified fraction and only to a lesser extent in the free cholesterol fraction. The changes in concentrations were accompanied by similar but less pronounced rises in high-density lipoprotein phospholipid and in apolipoprotein AI concentrations (p less than 0.01), whereas high-density lipoprotein phospholipid and in apolipoprotein AI concentration (p less than 0.01), whereas high-density lipoprotein triglyceride concentrations did not change significantly. These findings confirm and extend those of earlier cross-sectional studies which showed low concentrations of high-density lipoproteins in cigarette smokers, A significant correlation between the rise in high-density lipoprotein cholesterol concentrations and the increase in fat consumption after stopping smoking indicate that the changes in high-density lipoprotein concentrations may be partly due to nutritional factors.
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| 3164. |
- Stubbe, Ingo, et al.
(författare)
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In-hospital exercise therapy in patients with severe angina pectoris
- 1983
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Ingår i: Archives of Physical Medicine and Rehabilitation. - 0003-9993. ; 64:9, s. 396-401
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Tidskriftsartikel (refereegranskat)abstract
- The authors evaluated physiologic, psychologic and metabolic effects of a nine-week in-hospital training program on 14 men with severe disabling angina pectoris. The exercise program consisted of intensive interval training on an ergometer bicycle for two 30 min sessions daily. The physical performance increased by about 40% (p less than 0.001). Plasma insulin levels were reduced and glucose tolerance improved significantly. There was a decrease in plasma triglyceride and low-density lipoprotein (LDL) cholesterol levels, but no change in high-density lipoprotein (HDL) cholesterol, apolipoprotein AI and B concentrations. Plasma triglyceride (p less than 0.05) and LDL cholesterol (p less than 0.05) levels remained low three weeks after completion of the training period and the physical performance remained improved (p less than 0.01) even six months post-training. Four of the patients who had been disabled for at least five months were able to return to work. The authors suggest that comparatively short and intensive in-hospital rehabilitation of patients with coronary heart disease may be an attractive alternative to prolonged training on an outpatient basis, especially in patients with severe angina pectoris.
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| 3165. |
- Stubbe, Ingo, et al.
(författare)
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Plasma lipoproteins and lipolytic enzyme activities during endurance training in sedentary men: changes in high-density lipoprotein subfractions and composition
- 1983
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Ingår i: Metabolism, Clinical and Experimental. - : Elsevier BV. - 1532-8600. ; 32:12, s. 1120-1128
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Tidskriftsartikel (refereegranskat)abstract
- Eighteen healthy sedentary males took part in supervised bicycle training for 50 minutes three to five times a week. Twelve subjects (group A) trained for 6 weeks at heavy intensity, and six subjects (group B) trained for 12 weeks at moderate intensity. Maximal oxygen uptake increased by about 20% (P less than 0.01). Body weight and composition as well as diet remained unchanged. After 6 weeks plasma high-density lipoprotein (HDL) cholesterol concentrations had increased by 7% (P less than 0.05) in all subjects. The increase was most marked in group B at 14% (P less than 0.05) compared to 3% in group A (ns). Apolipoprotein AI (apo AI) increased by about 7% in both groups (P less than 0.01). After 12 weeks HDL cholesterol and apo AI levels had almost returned to initial values. Measurements of HDL components showed increases of 6% to 12% in free cholesterol, cholesteryl ester (P less than 0.05), and phospholipid (P less than 0.01); whereas, the minor triglyceride fraction decreased by 20% (P less than 0.01). Zonal ultracentrifugation in four subjects revealed a preferential rise of about 35% in the HDL2 subfraction, increasing the HDL2/HDL3 ratio by about 20%. In parallel, the composition of the lipoprotein classes changed. The protein moiety of all classes, except low-density lipoprotein (LDL), expanded at the expense of the core components cholesteryl ester and triglyceride. Hepatic lipase (HL) activity decreased by 6% (P less than 0.05), and lipoprotein lipase (LPL) activity in adipose tissue increased by about 50% (P less than 0.05) during the first 6 weeks of training, while LPL activity in postheparin plasma and skeletal muscle did not change. The transient rise in HDL cholesterol levels was correlated (P less than 0.05) to the elevation of adipose tissue LPL activity. The alterations in HDL concentration were also related to changes in body composition and diet, especially to an increase in fat intake.
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| 3166. |
- Sundberg, Mårten
(författare)
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Protein microarrays for validation of affinity binders
- 2011
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Is specificity an important issue regarding affinity reagents? What about the validation of affinity reagents today, is it good enough? This depends on the application and the producer of the reagent. Validation should be the most important marketing argument that can be found.Today there is a continuous growth of both the number of affinity reagents that are produced and the different types of affinity reagents that are developed. In proteomics they become more and more important in exploring the human proteome. Therefore, validated affinity reagents should be on top of every proteomic researcher’s list. How should this be accomplished?Better international agreements on how affinity reagents should be tested to be regarded as functional reagents are needed. One of the most important issues is the specificity of the affinity reagent. An international standard for which specific validation that is needed for different kinds of applications would be very useful.In this thesis, it is shown that the protein microarray platform that was established within the HPA project at KTH is a very good tool to determine the specificity of different affinity binders.In the first study, the production of mono-specific antibodies for tissue profiling in the Human Protein Atlas (HPA) project is presented. The section describing the use of protein microarrays for validation of the antibodies is relevant for this thesis. The implementation of protein microarrays in the HPA workflow was an important addition, because a deeper insight of the specificity of all the antibodies produced were now available.In a second study, bead based arrays were compared to planar protein microarrays used in the HPA project. In this study, 100 different bead identities were coupled with 100 different antigens and mixed together to generate an array. The correlation between the two types of assays was very high and the conclusion was that the methods can be used as backup to each other.A third study was a part of an international initiative to produce renewable affinity binders against proteins containing SH2 domain. Here, the HPA protein microarrays were modified to analyze different types of reagents produced at six laboratories around the world. Monoclonal antibodies, single chain fragment and fibronectin scaffolds were tested as well as mono-specific antibodies. It was shown to be possible to adapt protein microarrays used in the HPA project to validate other kinds of affinity reagents.
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| 3167. |
- Sundqvist, Pernilla, 1979-, et al.
(författare)
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Finns det ett glapp i förskolans praktik när det kommer till teknik?
- 2018
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Konferensbidrag (refereegranskat)abstract
- Tidigare forskning indikerar att förskolepedagogers beskrivning av teknik som område generellt inte återfinns i förskolans arbete. Här undersöks om ett sådant glapp kan påvisas. Studien har bedrivits som forskningscirklar med 19 förskolepedagoger. Utgångspunkten var en enkät vars resultat beskriver teknik i fem kategorier. Därefter har pedagogerna dokumenterat exempel med tekniklärande för barnen och angett vilken av teknikkategorierna som bäst beskriver tekniken i händelsen. I enkäten dominerade kategorierna att ”teknik är lösning på att problem” och ”processen i vilken artefakter utvecklas”. I exemplen finns omvänt aktiviteter som knyter an till att identifiera artefakter och att använda teknikord och artefakter. Skillnaden mellan pedagogernas beskrivning av teknik och praktiken ger de själva en relevant förklaring till.
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| 3168. |
- Sundqvist, Pernilla, 1979-
(författare)
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Förskolans teknikundervisning: vad och hur?
- 2019
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The aim of this study was to provide a description of the subject area of technology and the teaching of it in preschool. Three research questions were addressed: 1) What is the content of the subject area of technology in preschool? 2) How do the participants in the study teach technology in preschool? and 3) What aspects of technology does the preschool staff enable the children to learn? The motivation behind the study is the fact that technology is a relatively new teaching area in preschool. Research on the topic is scarce and does not provide preschool staff with an adequate scientific basis on which to build their teaching. Data were generated in three phases. First, a questionnaire was sent to 10 % of preschools in one municipality, asking staff what they include in the subject area of technology and how they view the teaching of technology in preschool. Secondly, seven preschool staff who had responded to the questionnaire were interviewed with the aim of providing a more detailed description of how technology is taught in their respective preschools. Thirdly, an ethnographically inspired perspective was employed with the participation of two preschool units. I visited them during a six-week period, carried out observations and interviewed staff. The outcome of the research is presented in the form of five individual studies, which collectively provided answers to the research questions. The results showed that preschool staff vary in their descriptions of the content of the technology area and how it is taught, with a range spanning from content that does not belong in technology as a knowledge area to relatively complex technological content. Examples of the former were content such as natural science, non-technological skills and techniques and the use of technology as a means of learning in other subject areas. Examples of the latter were content such as how a specific technology works, for instance what parts an object is made of and how they are joined together, as well as knowledge of technological systems. The descriptions revealed varying levels of competence in the teaching of technology to preschool children, with some staff showing adequate knowledge and confidence and others showing a lower level of knowledge as well as insecurity. It also becomes clear that the intended equality in preschool education had not been achieved in respect of technology education. From the part of this study that was based on an ethnographically inspired perspective it is clear that when preschool staff have the interest and the knowledge needed to teach technology to children, such teaching can be meaningful to the children and contribute to their learning in accordance with the stated mission of the preschool. The study has relevance for preschool practice as well as for the research community.
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| 3169. |
- Sundqvist, Pernilla, 1979-, et al.
(författare)
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Is There a Gap to Mind in Preschool Practice When it Comes to Technology?
- 2018
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Ingår i: XVIII IOSTE symposium.. - Malmö : Malmö University. - 9789171049711 ; , s. 303-310
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Konferensbidrag (refereegranskat)abstract
- Research has indicated that there may be a gap between preschool teachers’ general descriptions of technology and thetechnology content in their actual preschool practices. This study investigates this further and, if a gap is found, looks forpossible reasons for it. The study was conducted in the form of research circles in two Swedish municipalities with 19technology-minded preschool teachers. A mixed research design was used. The starting point for mapping the preschoolteachers’ descriptions of technology was an established questionnaire that placed technology into five categories. Followingthis, the teachers independently documented events at their preschools that they assessed as technology activities. The findingswere that the most commonly chosen category in the questionnaire was technology as a solution to a problem. However, intheir everyday examples the dominant activities related to the children’s attempts to use the artefacts. The preschool teacherssaid that the children had to be introduced to the artefacts, their names and functions, before moving on to more advancedlevels describing technology. There is thus a distinction between the preschool teachers’ descriptions of technology and theireveryday practices, where they themselves provide relevant explanations for the gap.
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| 3170. |
- Sundqvist, Pernilla, et al.
(författare)
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Svensk förskolepersonals beskrivningar av teknik
- 2015
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Ingår i: LUMAT. - LUMA Centre Finland. - 2323-7112. ; 3:2, s. 237-257
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Tidskriftsartikel (refereegranskat)abstract
- With regards to previous research results on how the teacher’s/preschool teacher’s understanding of a subject effects children’s learning and apprehending of the subject combined with the difficulties for the technology subject to take place in preschool this study aims at investigating how preschool staff (including preschool teachers and day care attendants) describe technology. A qualitatively designed questionnaire was sent out to 139 preschool teachers and day care attendants in a Swedish municipality, whereof 102 answered. The open question about what technology is thought to be was analyzed using a conventional content analysis resulting in nine categories, of which six interrelated hierarchically and one contained answers witch could not be categorized. Thus the result shows eight different ways in which technology is described, from a simple and naive description to a more complex description that implicates a deeper understanding of technology. A majority describes technology according to the latter. The result also shows that it is mainly the preschool teachers who describe technology in a more complex way. Many of the respondents have trouble separating technology from science, and quite a few describes technology as technique, which is another meaning for the word in the Swedish language.
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