| 921. |
- Welinder, Charlotte, et al.
(author)
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Establishing a Southern Swedish Malignant Melanoma OMICS and Biobank Clinical Capability
- 2013
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In: Clinical and Translational Medicine. - : Wiley. - 2001-1326. ; 2:1
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Journal article (peer-reviewed)abstract
- Background: The Southern Swedish Malignant Melanoma (SSMM) research team is a truly cross functional group with members from oncology, clinical, surgery, bioinformatics, proteomics, and genomics initiatives. The SSMM’s objectives and goals are to develop, build and utilize cutting edge biobanks and OMICS platforms to better understand disease pathology and drug mechanisms. Within the research team there are members who daily diagnose patients with suspect melanomas, do follow-ups on malignant melanoma patients and remove primary or metastatic lesions by surgery. This inter-disciplinary clinical patient care ensures a competence build as well as a best practice procedure where the patient benefits. The science output in these resulting study outcomes further strengthens the build of healthcare benefit in the complex challenges of malignant melanoma pathophysiology that is addressed by the novel personalized medicines entering the market. These patient biobank archives will be fully automated with novel ultralow temperature biobank storage units and used as a clinical resource. Methods: Clinical materials from patients before, during and after treatments, with clinical end points are being collected. Tissue samples as well as bio-fluid samples such as blood fractions, plasma, serum and whole blood will be archived in 384-high density sample tube formats. We are developing standardized approaches for patient selections, patient sampling, sample-processing and analysis platforms with dedicated protein assays and genomics platforms that will hold value for the research community. Results: An IT-infrastructure using a laboratory information management system (LIMS) has been established, that will be the key interface for the research teams in order to share and explore data generated within the project. The cross-site data repository in Lund will form the basis for sample processing, together with biological samples in southern Sweden, including blood fractions and tumor tissues. Clinical registries are being associated with the biobank materials, including pathology reports on disease diagnosis on the MM patients. Conclusions: We provide data on the developments of protein profiling and targeted protein target assays on isolated melanoma tumors, as well as reference blood standards that is used by the team members in the respective laboratories. These pilot data show biobank access and feasibility of performing quantitative proteomics in MM biobank repositories collected in southern Sweden.
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| 922. |
- Welinder, Charlotte, et al.
(author)
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Expression of Helix pomatia Lectin Binding Glycoproteins in Women with Breast Cancer in Relationship to Their Mood Group Phenotypes
- 2009
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In: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 8:2, s. 782-786
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Journal article (peer-reviewed)abstract
- Aberrant glycosylation occurs in essentially all types of human cancers. A difference in glycopattern of proteins will result in a change of function of the proteins. The lectin from Helix pomatia (HPA) recognizes N-acetylgalactosaminylated glycoproteins and very consistent results over the increased binding of HPA in tissue sections are associated with metastasis progression and poor patient prognosis in a range of human adenocarcinomas. The induced modification of protein function after changed glycosylation is unknown, and as a part in characterizing the glycoproteins carrying the specific carbohydrates, we analyzed the major HPA binding proteins in sera from healthy women, women with primary breast cancer with no metastasis (bcmet-), and women with metastasizing breast cancer (bcmet+) using lectin affinity chromatography and lectin blotting. The binding ligands were further identified using mass spectrometry (MALDI-TOF MS) to confirm the captured glycoproteins. The major HPA binding proteins in serum were found to be IgA1, complement factor C3, von Willebrand factor (vWF), alpha-2-macroglobulin and IgM. This set of antigens is a panel of candidates for useful HPA related biomarkers in sera, but our results also emphasize the fact that the blood group phenotypes are of most importance when using the lectin HPA in recognition of cancer biomarkers in sera and plasma. The results emphasize that interpretation of an individual change in the glycosylation pattern of a specific tumor marker always needs to be analyzed in its right context. This study shows that the blood group phenotypes can have a major impact on the results when analyzing HPA lectin binding.
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| 923. |
- Welinder, Charlotte, et al.
(author)
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Feasibility Study on Measuring Selected Proteins in Malignant Melanoma Tissue by SRM Quantification.
- 2014
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In: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 13:3, s. 1315-1326
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Journal article (peer-reviewed)abstract
- Currently there are no clinically recognized molecular biomarkers for malignant melanoma (MM) for either diagnosing disease stage or measuring response to therapy. The aim of this feasibility study was to develop targeted selected reaction monitoring (SRM) assays for identifying candidate protein biomarkers in metastatic melanoma tissue lysate. In a pilot study applying the SRM assay, the tissue expression of nine selected proteins [complement 3 (C3), T-cell surface glycoprotein CD3 epsilon chain E (CD3E), dermatopontin, minichromosome maintenance complex component (MCM4), premelanosome protein (PMEL), S100 calcium binding protein A8 (S100A8), S100 calcium binding protein A13 (S100A13), transgelin-2 and S100B] was quantified in a small cohort of metastatic malignant melanoma patients. The SRM assay was developed using a TSQ Vantage triple quadrupole mass spectrometer that generated highly accurate peptide quantification. Repeated injection of internal standards spiked into matrix showed relative standard deviation (RSD) from 6% to 15%. All nine target proteins were identified in tumor lysate digests spiked with heavy peptide standards. The multiplex SRM peptide assay panel was then measured and quantified on a set of frozen MM tissue samples obtained from the Malignant Melanoma Biobank collected in Lund, Sweden. All nine proteins could be accurately quantified using the new SRM assay format. This study provides preliminary data on the heterogeneity of biomarker expression within MM patients. The S100B protein, which is clinically used as the pathology identifier of MM, was identified in 9 out of 10 MM tissue lysates. The use of the targeted SRM assay provides potential advancements in the diagnosis of MM that can aid in future assessments of disease in melanoma patients.
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| 924. |
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| 925. |
- Wendin, Karin, et al.
(author)
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Mat, Näring och Robotar
- 2014
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In: Dietistaktuellt. ; 23:5, s. 18-20
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Journal article (pop. science, debate, etc.)
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| 926. |
- Wendin, Karin, et al.
(author)
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Mat, Näring och Robotar
- 2014
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In: Dietistaktuellt. ; 23:5, s. 18-20
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Journal article (other academic/artistic)
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| 927. |
- Wennerström, Håkan, et al.
(author)
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Entropic forces between fluid layers.
- 2013
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In: Proceedings of the National Academy of Sciences. - : Proceedings of the National Academy of Sciences. - 1091-6490 .- 0027-8424. ; 110:32, s. 2944-2944
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Journal article (peer-reviewed)
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| 928. |
- Wennerström, Håkan, et al.
(author)
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Interfacial tensions in microemulsions
- 2006
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In: Colloids and Surfaces A: Physicochemical and Engineering Aspects. - : Elsevier BV. - 0927-7757. ; 291:1-3, s. 69-77
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Journal article (peer-reviewed)abstract
- We present a theoretical analysis of the interfacial tensions of microemulsions in ternary systems water-oil-nonionic surfactant. For surfactants based on oligo-ethylene oxide head-groups the temperature can be used to tune the properties of the microemulsions. Explicit expressions are derived for the interfacial tension of both droplet and bicontinuous microemulsions. We also discuss the effect of both capillary waves and bending modes on the interfacial tensions. Sottman and Strey [T. Soumann, R. Strey, Ultralow interfacial tensions in water-n-alkane-surfactant systems, J. Chem. Phys. 106 (1997) 8606-8615] have measured interfacial tensions for a range of systems for temperatures covering Winsor I to Winsor III to Winsor II phase behavior. The theoretical expressions are consistent with the experimental data. Under most conditions it is the curvature energy that gives the dominant contribution to the interfacial tension. The magnitude of the effect is determined by different elastic constants for droplet and bicontinuous microemulsions and we conclude that very careful measurements of interfacial tensions can be used to determine both the bending elastic constants of the Helfrich curvature free energy. (c) 2006 Elsevier B.V. All rights reserved.
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| 929. |
- Wennerström, Håkan, et al.
(author)
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Microemulsions as model systems
- 2009
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In: Comptes Rendus. Chimie. - : Elsevier BV. - 1631-0748. ; 12:1-2, s. 4-17
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Journal article (peer-reviewed)abstract
- It is demonstrated how microemulsions can be used as model systems to study a range of fundamental liquid state phenomena. By carefully characterizing a single ternary system it is possible to obtain both a qualitative understanding and a quantitative description using the concept of the curvature energy of the surfactant film at the water-oil interface. We demonstrate the approach on the following problems: calculation of the oil-water interfacial free energy; nucleation; Ostwald ripening; solubilization kinetics; the Hofmeister effect; macroemulsion stability; vesicle fusion and fission. For all these phenomena it is demonstrated that one can by combining experimental studies on water-oil-nonionic surfactant systems with a careful theoretical analysis obtain an understanding that goes beyond what has been achieved using conventional systems. To cite this article: H. Wennerstrom, U. Olsson, C R. Chimie 12 (2009). (C) 2008 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.
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| 930. |
- Wennerström, Håkan, et al.
(author)
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The undulation force; theoretical results versus experimental demonstrations.
- 2014
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In: Advances in Colloid and Interface Science. - : Elsevier BV. - 1873-3727 .- 0001-8686. ; 208:Online 19 February 2014, s. 10-13
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Journal article (peer-reviewed)abstract
- In 1978 Wolfgang Helfrich published a paper (Helfrich W. Z Naturforsch 1978; 33a:305) where he introduced the notion of an undulation force between bilayers. This is caused by thermal excitations of the bending modes being restricted by the presence of neighboring layers. Although there is now a consensus on the qualitative picture put forward by Helfrich there is still a debate concerning the quantitative aspects. We discuss in particular the distance dependence of the interaction and also the value of the numerical coefficient of the force law derived by Helfrich.
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