| 381. |
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| 382. |
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| 383. |
- Kõljalg, Urmas, et al.
(författare)
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Towards a unified paradigm for sequence-based identification of fungi.
- 2013
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Ingår i: Molecular ecology. - : Wiley. - 1365-294X .- 0962-1083. ; 22:21, s. 5271-7
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Tidskriftsartikel (refereegranskat)abstract
- The nuclear ribosomal internal transcribed spacer (ITS) region is the formal fungal barcode and in most cases the marker of choice for the exploration of fungal diversity in environmental samples. Two problems are particularly acute in the pursuit of satisfactory taxonomic assignment of newly generated ITS sequences: (i) the lack of an inclusive, reliable public reference data set and (ii) the lack of means to refer to fungal species, for which no Latin name is available in a standardized stable way. Here, we report on progress in these regards through further development of the UNITE database (http://unite.ut.ee) for molecular identification of fungi. All fungal species represented by at least two ITS sequences in the international nucleotide sequence databases are now given a unique, stable name of the accession number type (e.g. Hymenoscyphus pseudoalbidus|GU586904|SH133781.05FU), and their taxonomic and ecological annotations were corrected as far as possible through a distributed, third-party annotation effort. We introduce the term 'species hypothesis' (SH) for the taxa discovered in clustering on different similarity thresholds (97-99%). An automatically or manually designated sequence is chosen to represent each such SH. These reference sequences are released (http://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and in the QIIME pipeline. The system and the data will be updated automatically as the number of public fungal ITS sequences grows. We invite everybody in the position to improve the annotation or metadata associated with their particular fungal lineages of expertise to do so through the new Web-based sequence management system in UNITE.
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| 384. |
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| 385. |
- Kuligowski, Erica D., et al.
(författare)
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Movement on Stairs During Building Evacuations
- 2014
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The time that it takes an occupant population to reach safety when descending a stair during building evacuations is typically estimated by measureable engineering variables such as stair geometry, speed, stair density, and pre-observation delay. In turn, engineering models of building evacuation use these variables to predict the performance of egress systems for building design, emergency planning, or event reconstruction. As part of a program to better understand occupant movement and behavior during building emergencies, the Engineering Laboratory at the National Institute of Standards and Technology (NIST) has been collecting stair movement data during fire drill evacuations of office and residential buildings. These data collections are intended to provide a better understanding of this principal building egress feature and develop a technical foundation for future codes and standards requirements. NIST has collected fire drill evacuation data in 14 buildings (11 office buildings and 3 residential buildings) ranging from six to 62 stories in height that have included a range of stair widths and occupant densities. A total of more than 22000 individual measurements are included in the data set. This report provides details of the data collected, an analysis of the data, and examples of the use of the data. The intention is to better understand movement during stair evacuations and provide data to test the predictive capability of building egress models. While mean movement speeds in the current study of 0.44 m/s ± 0.19 m/s are observed to be quite similar to the range of values in previous studies, mean local movement speeds as occupants traverse down the stairs are seen to vary widely within a given stair, ranging from 0.10 m/s ± 0.008 m/s to 1.7 m/s ± 0.13 m/s. These data provide confirmation of the adequacy of existing literature values typically used for occupant movement speeds and provide updated data for use in egress modeling or other engineering calculations.
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| 386. |
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| 387. |
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| 388. |
- Kuschnig, R., Ryabchikova, T.A., Piskunov, N., Weiss, W.W., LeContel, J.M.
(författare)
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The atmosphere of the peculiar binary system ET Andromedae
- 1995
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Ingår i: Astronomy & Astrophysics. - : EDP Sciences. - 0004-6361. ; 294:3, s. 757-
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Tidskriftsartikel (refereegranskat)abstract
- High spectral resolution, high signal-to-noise OHP spectra which cover the range from 4000A to 7500A were used to derive abundances for ET And. An extreme overabundance of silicon by nearly 100 times of the solar value corroborates the Si-star classificat
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| 389. |
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| 390. |
- Leibovitzh, Haim, et al.
(författare)
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Immune response and barrier dysfunction-related proteomic signatures in preclinical phase of Crohn's disease highlight earliest events of pathogenesis
- 2023
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Ingår i: Gut. - : BMJ Publishing Group Ltd. - 0017-5749 .- 1468-3288. ; 72:8, s. 1462-1471
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: The measure of serum proteome in the preclinical state of Crohn's disease (CD) may provide insight into biological pathways involved in CD pathogenesis. We aimed to assess associations of serum proteins with future CD onset and with other biomarkers predicting CD risk in a healthy at-risk cohort.DESIGN: In a nested case-control study within the Crohn's and Colitis Canada Genetics Environment Microbial Project (CCC-GEM) cohort, which prospectively follows healthy first-degree relatives (FDRs), subjects who developed CD (n=71) were matched with four FDRs remaining healthy (n=284). Using samples at recruitment, serum protein profiles using the Olink Proximity Extension Assay platform was assessed for association with future development of CD and with other baseline biomarkers as follows: serum antimicrobial antibodies (AS: positive antibody sum) (Prometheus); faecal calprotectin (FCP); gut barrier function using the fractional excretion of lactulose-to-mannitol ratio (LMR) assay.RESULTS: We identified 25 of 446 serum proteins significantly associated with future development of CD. C-X-C motif chemokine 9 (CXCL9) had the highest OR with future risk of CD (OR=2.07 per SD, 95% CI 1.58 to 2.73, q=7.9e-5), whereas matrix extracellular phosphoglycoprotein had the lowest OR (OR 0.44, 95% CI 0.29 to 0.66, q=0.02). Notably, CXCL9 was the only analyte significantly associated with all other CD-risk biomarkers with consistent direction of effect (FCP: OR=2.21; LMR: OR=1.67; AS: OR=1.59) (q<0.05 for all).CONCLUSION: We identified serum proteomic signatures associated with future CD development, reflecting potential early biological processes of immune and barrier dysfunction.
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