Rapid degradation of the presequence of the F-1 beta precursor of the ATP synthase inside mitochondria

• We have investigated the fate of the presequence of an overexpressed protein derived from the precursor of the F(1)beta subunit of ATP synthase after import and processing in mitochondria. Our studies revealed a rapid degradation of the presequence inside mitochondria catalysed by matrix-located protease(s). In contrast, the mature portion of the precursor was not degraded. This is the first experimental evidence of the rapid degradation of the F(1)beta a mitochondrial presequence in organello after in vitro import and processing.

Nyckelord

protein import

proteolysis

signal peptide

nitric-oxide synthase

protein import

processing peptidase

thimet oligopeptidase

synthetic presequence

yeast mitochondria

targeting peptides

cleavage

sequence

binding

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)