| 1. |
- Linhult, M., et al.
(författare)
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Affinity ligands for industrial protein purification
- 2005
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Ingår i: Protein peptide letters. - : Bentham Science Publishers Ltd.. - 0929-8665 .- 1875-5305. ; 12:4, s. 305-310
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Forskningsöversikt (refereegranskat)abstract
- Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.
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| 2. |
- Tanhaian, Abbas, et al.
(författare)
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In Silico and In Vitro Investigation of a Likely Pathway for Anti-Cancerous Effect of Thrombocidin-1 as a Novel Anticancer Peptide
- 2020
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Ingår i: Protein peptide letters. - : Bentham Science Publishers Ltd. - 0929-8665 .- 1875-5305. ; 27:8, s. 751-762
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Tidskriftsartikel (refereegranskat)abstract
- Background: Antimicrobial and antifungal activities of Thrombocidin-1 (TC-1) is shown previously, however, the anti-cancerous feature of this peptide is still uncovered. Objective: The objective is to evaluate anti-cancerous feature of recombinant TC-1. Methods: In this study, based on the significant similarity of rTC-1 and IL-8 in case of coding sequence, tertiary structure, and also docking and molecular dynamic simulation (MD) results with CXCR1, a receptor which has positive correlation with different cancers, a likely pathway for anti-cancerous effect of rTC-1 was proposed. In addition, the coding sequence of TC-1+6 x histidine (rTC-1) was inserted into the pET22b(+) vector and cloned and expressed by E. coli BL21 and finally purified through nickel affinity column. Afterward, the retrieved rTC-1 was used in MTT assay against mouse colon adenocarcinoma, hepatocellular carcinoma, chondrosarcoma, mouse melanoma, and breast adenocarcinoma cell lines to investigate its probable anticancer application. Results: Docking and MD simulation results showed that rTC-1 and IL-8 share almost the same residues in the interaction with CXCR1 receptor. Besides, the stability of the rTC-l_CXCR1(1-)(38) complex was shown during 100ns MD simulation. In addition, the successful expression and purification of rTC-1 depict an 8kD peptide. The IC50 results of MTT assay revealed that rTC-1 has cytotoxic effect on C26-A and SW1353 cancerous cell lines. Conclusion: Therefore, apart from probable anti-cancerous effect of rTC-1 on C26-A and SW1353 cell lines, this peptide may be able to mimic the anti-cancerous pathway of IL-8.
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| 3. |
- Belleschi, Marco, et al.
(författare)
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Benchmarking Practical RRM Algorithms for D2D Communications in LTE Advanced
- 2014
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Ingår i: Wireless personal communications. - : Springer. - 0929-6212 .- 1572-834X. ; 82:2, s. 883-910
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Tidskriftsartikel (refereegranskat)abstract
- Device-to-device (D2D) communication integrated into cellular networks is an advanced tool to take advantage of the proximity of devices and allow for reusing cellular resources and thereby to increase the user bitrates and the system capacity. However, the introduction of D2D in legacy long term evolution (LTE) cellular spectrum requires to revisit and modify the existing radio resource management and power control (PC) techniques in order to fully realize the potential of the proximity and reuse gains and to limit the interference to the cellular layer. In this paper, we examine the performance of the legacy LTE PC tool box and benchmark it against an utility optimal iterative scheme. We find that the open loop PC scheme of LTE performs well for cellular users both in terms of the used transmit power levels and the achieved signal-to-interference-and-noise-ratio distribution. However, the performance of the D2D users as well as the overall system throughput can be boosted by the utility optimal scheme, by taking better advantage of both the proximity and the reuse gains. Therefore, in this paper we propose a hybrid PC scheme, in which cellular users employ the legacy LTE open loop PC, while D2D users exploits the utility optimizing distributed PC scheme. We also recognize that the hybrid scheme is not only nearly optimal, and can balance between spectral and energy efficiency, but it also allows for a distributed implementation at the D2D users, while preserving the LTE PC scheme for the cellular users.
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