| 1. |
- Barriga, Hanna M. G., et al.
(author)
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Coupling Lipid Nanoparticle Structure and Automated Single-Particle Composition Analysis to Design Phospholipase-Responsive Nanocarriers
- 2022
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In: Advanced Materials. - : Wiley. - 0935-9648 .- 1521-4095. ; 34:26
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Journal article (peer-reviewed)abstract
- Lipid nanoparticles (LNPs) are versatile structures with tunable physicochemical properties that are ideally suited as a platform for vaccine delivery and RNA therapeutics. A key barrier to LNP rational design is the inability to relate composition and structure to intracellular processing and function. Here Single Particle Automated Raman Trapping Analysis (SPARTA) is combined with small-angle X-ray and neutron scattering (SAXS/SANS) techniques to link LNP composition with internal structure and morphology and to monitor dynamic LNP-phospholipase D (PLD) interactions. This analysis demonstrates that PLD, a key intracellular trafficking mediator, can access the entire LNP lipid membrane to generate stable, anionic LNPs. PLD activity on vesicles with matched amounts of enzyme substrate is an order of magnitude lower, indicating that the LNP lipid membrane structure can be used to control enzyme interactions. This represents an opportunity to design enzyme-responsive LNP solutions for stimuli-responsive delivery and diseases where PLD is dysregulated.
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| 2. |
- Berggren, Magnus, et al.
(author)
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Organic bioelectronics
- 2007
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In: Advanced Materials. - : Wiley. - 0935-9648 .- 1521-4095. ; 19:20, s. 3201-3213
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Research review (peer-reviewed)abstract
- During the last two decades, organic electroactive materials have been explored as the working material in a vast array of electronic devices, promising low-cost, flexible, and easily manufactured systems. The same materials also possess features that make them unique in bioelectronics applications, where electronic signals are translated into biosignals and vice versa. Here we review, in the broadest sense, the field of organic bioelectronics, describing the electronic properties and mechanisms of the organic electronic materials that are utilized in specific biological experiments.
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| 3. |
- Bolin, Maria, et al.
(author)
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Active Control of Epithelial Cell-Density Gradients Grown Along the Channel of an Organic Electrochemical Transistor
- 2009
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In: ADVANCED MATERIALS. - : Wiley. - 0935-9648 .- 1521-4095. ; 21:43, s. 4379-
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Journal article (peer-reviewed)abstract
- Complex patterning of the extracellular matrix, cells, and tissues under in situ electronic control is the aim of the technique presented here. The distribution of epithelial cells along the channel of an organic electrochemical transistor is shown to be actively controlled by the gate and drain voltages, as electrochemical gradients are formed along the transistor channel when the device is biased.
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| 4. |
- Chen, Song, et al.
(author)
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Formation of Amorphous Iron-Calcium Phosphate with High Stability
- 2023
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In: Advanced Materials. - : John Wiley & Sons. - 0935-9648 .- 1521-4095. ; 35:33
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Journal article (peer-reviewed)abstract
- Amorphous iron-calcium phosphate (Fe-ACP) plays a vital role in the mechanical properties of teeth of some rodents, which are very hard, but its formation process and synthetic route remain unknown. Here, the synthesis and characterization of an iron-bearing amorphous calcium phosphate in the presence of ammonium iron citrate (AIC) are reported. The iron is distributed homogeneously on the nanometer scale in the resulting particles. The prepared Fe-ACP particles can be highly stable in aqueous media, including water, simulated body fluid, and acetate buffer solution (pH 4). In vitro study demonstrates that these particles have good biocompatibility and osteogenic properties. Subsequently, Spark Plasma Sintering (SPS) is utilized to consolidate the initial Fe-ACP powders. The results show that the hardness of the ceramics increases with the increase of iron content, but an excess of iron leads to a rapid decline in hardness. Calcium iron phosphate ceramics with a hardness of 4 GPa can be achieved, which is higher than that of human enamel. Furthermore, the ceramics composed of iron-calcium phosphates show enhanced acid resistance. This study provides a novel route to prepare Fe-ACP, and presents the potential role of Fe-ACP in biomineralization and as starting material to fabricate acid-resistant high-performance bioceramics.
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| 7. |
- Kavand, Hanie, et al.
(author)
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3D‐Printed Biohybrid Microstructures Enable Transplantation and Vascularization of Microtissues in the Anterior Chamber of the Eye
- 2023
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In: Advanced Materials. - : Wiley. - 0935-9648 .- 1521-4095.
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Journal article (peer-reviewed)abstract
- Hybridizing biological cells with man-made sensors enable the detection of a wide range of weak physiological responses with high specificity. The anterior chamber of the eye (ACE) is an ideal transplantation site due to its ocular immune privilege and optical transparency, which enable superior non-invasive longitudinal analyses of cells and microtissues. Engraftment of biohybrid microstructures in the ACE might, however, be affected by the pupillary response and dynamics. Here, sutureless transplantation of biohybrid microstructures, 3D printed in IP-Visio photoresin, containing a precisely localized pancreatic islet to the ACE of mice is presented. The biohybrid microstructures allow mechanical fixation in the ACE, independent of iris dynamics. After transplantation, islets in the microstructures successfully sustain their functionality for over 20 weeks and become vascularized despite physical separation from the vessel source (iris) and immersion in a low-viscous liquid (aqueous humor) with continuous circulation and clearance. This approach opens new perspectives in biohybrid microtissue transplantation in the ACE, advancing monitoring of microtissue-host interactions, disease modeling, treatment outcomes, and vascularization in engineered tissues.
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| 8. |
- Kavand, Hanie, et al.
(author)
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Advanced Materials and Sensors for Microphysiological Systems: Focus on Electronic and Electro‐optical Interfaces
- 2021
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In: Advanced Materials. - : Wiley. - 0935-9648 .- 1521-4095. ; , s. 2107876-2107876
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Journal article (peer-reviewed)abstract
- Advanced in vitro cell culture systems or microphysiological systems (MPSs), including microfluidic organ-on-a-chip (OoC), are breakthrough technologies in biomedicine. These systems recapitulate features of human tissues outside of the body. They are increasingly being used to study the functionality of different organs for applications such as drug evolutions, disease modeling, and precision medicine. Currently, developers and endpoint users of these in vitro models promote how they can replace animal models or even be a better ethically neutral and humanized alternative to study pathology, physiology, and pharmacology. Although reported models show a remarkable physiological structure and function compared to the conventional two-dimensional cell culture, they are almost exclusively based on standard passive polymers or glass with none or minimal real-time stimuli and readout capacity. The next technology leap in reproducing in vivo-like functionality and real-time monitoring of tissue function could be realized with advanced functional materials and devices. This review describes the currently reported electronic and optical advanced materials for sensing and stimulation of MPS models. In addition, we give an overview of multi-sensing for Body-on-Chip platforms. Finally, we give our perspective on how advanced functional materials could be integrated into in vitro systems to precisely mimic human physiology.
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