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Generation of monos...
Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
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- Hjelm, Barbara (författare)
- KTH,Albanova VinnExcellence Center for Protein Technology, ProNova
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- Forsström, Björn (författare)
- KTH,Albanova VinnExcellence Center for Protein Technology, ProNova
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- Igel, Ulrika (författare)
- KTH,Science for Life Laboratory, SciLifeLab
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Johannesson, Henrik (författare)
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- Stadler, Charlotte (författare)
- KTH,Science for Life Laboratory, SciLifeLab
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- Lundberg, Emma (författare)
- KTH,Science for Life Laboratory, SciLifeLab
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- Pontén, Fredrik (författare)
- Uppsala universitet,Molekylär och morfologisk patologi,Pontén
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Sjoberg, Anna (författare)
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- Rockberg, Johan (författare)
- KTH,Albanova VinnExcellence Center for Protein Technology, ProNova
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- Schwenk, Jochen M. (författare)
- KTH,Science for Life Laboratory, SciLifeLab
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- Nilsson, Peter (författare)
- KTH,Science for Life Laboratory, SciLifeLab
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Johansson, Christine (författare)
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- Uhlen, Mathias (författare)
- KTH,Albanova VinnExcellence Center for Protein Technology, ProNova,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2011-10-12
- 2011
- Engelska.
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Ingår i: Protein Science. - : Wiley. - 0961-8368 .- 1469-896X. ; 20:11, s. 1824-1835
- Relaterad länk:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- A method is described to generate and validate antibodies based on mapping the linear epitopes of a polyclonal antibody followed by sequential epitope-specific capture using synthetic peptides. Polyclonal antibodies directed towards four proteins RBM3, SATB2, ANLN, and CNDP1, potentially involved in human cancers, were selected and antibodies to several non-overlapping epitopes were generated and subsequently validated by Western blot, immunohistochemistry, and immunofluorescence. For all four proteins, a dramatic difference in functionality could be observed for these monospecific antibodies directed to the different epitopes. In each case, at least one antibody was obtained with full functionality across all applications, while other epitope-specific fractions showed no or little functionality. These results present a path forward to use the mapped binding sites of polyclonal antibodies to generate epitope-specific antibodies, providing an attractive approach for large-scale efforts to characterize the human proteome by antibodies.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- epitope mapping
- monospecific antibody
- affinity chromatography
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Hjelm, Barbara
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Forsström, Björn
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Igel, Ulrika
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Johannesson, Hen ...
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Stadler, Charlot ...
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Lundberg, Emma
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visa fler...
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Pontén, Fredrik
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Sjoberg, Anna
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Rockberg, Johan
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Schwenk, Jochen ...
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Nilsson, Peter
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Johansson, Chris ...
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Uhlen, Mathias
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visa färre...
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Protein Science
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