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Träfflista för sökning "L773:1549 3296 OR L773:1552 4965 ;pers:(Caldwell Karin D.)"

Sökning: L773:1549 3296 OR L773:1552 4965 > Caldwell Karin D.

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1.
  • Sandberg, Tomas, et al. (författare)
  • Potential use of mucins as biomaterial coatings. I. Fractionation, characterization, and model adsorption of bovine, porcine, and human mucins
  • 2009
  • Ingår i: Journal of Biomedical Materials Research Part A. - : Wiley. - 1549-3296 .- 1552-4965. ; 91A:3, s. 762-772
  • Tidskriftsartikel (refereegranskat)abstract
    • Previously, we presented evidence that mucins have potential as   biomaterial coatings. Here, we reveal substantial batch-to-batch   variations for a frequently used commercial bovine salivary mucin   preparation (BSM) and stress the importance of standardizing mucins   intended for comparative purposes. "Mild" fractionation strategies,   aiming at preserving natural mucin functions, were used to prepare two   more defined BSM fractions as well as three mucin fractions from   porcine gastric (PGM) and human salivary (MG1) sources. While the BSM   and PGM were highly purified and mainly adopted random coil   conformations in solution, the MG1 contained mucin-bound components   (1.6 wt% albumin) and appeared compact. Average molar masses and   root-mean-square radii for the predominant BSM, PGM, and MG1 species   spanned 0.8-4.2 MDa and 46-86 nm, respectively. An ellipsometric   evaluation, using hydrophilic and hydrophobic silica, showed the mucin   adsorption to be slow and related to mucin charge, size, conformation,   and compositional complexity. The mass uptakes on hydrophobic silica   averaged 2.6, 2.6, and 5.0 mg/m(2), for BSM, PGM, and MG1,   respectively. Finally, we find that stable mucin coatings can be formed   on polymers of different wettability. The reported mucin preparations   serve as platforms for a series of studies on the biocompatibility of  mucin coatings.
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2.
  • Sandberg, Tomas, et al. (författare)
  • Potential use of mucins as biomaterial coatings. II. Mucin coatings affect the conformation and neutrophil-activating properties of adsorbed host proteins – Towards a mucosal mimic
  • 2009
  • Ingår i: Journal of Biomedical Materials Research: Part A. - : Wiley. - 1549-3296 .- 1552-4965. ; 91A:3, s. 773-785
  • Tidskriftsartikel (refereegranskat)abstract
    • In continuation of our recent fractionation and characterization study   on mucins of bovine salivary (BSM), porcine gastric (PGM), and human   salivary (MG1) origin, this study evaluates the effect of mucin   precoating on the conformation and neutrophil-activating properties of   host proteins adsorbed to a polyethylene terephthalate-based model   biomaterial. Microscopy combined with assays for the neutrophil   releases of reactive oxygen species and human neutrophil lipocalin   showed that mucin precoating greatly reduced the strong immune-response   normally induced by adsorbed immunoglobulin G (IgG) and secretory   immunoglobulin A (sIgA), respectively. A similar finding was made for   the proinflammatory fibrinogen. Although the total uptakes of these   proteins depended on the mucin surface concentration, a detailed   composite analysis suggested the fraction Of surface-exposed protein to   be a stronger determinant of coating performance. The unexpectedly low   neutrophil activation showed by composites containing near-monolayer   concentrations of exposed IgG and sIgA, respectively, suggested that   these act synergistically with mucin on the surface. In support of this   hypothesis, quartz crystal microbalance with dissipation monitoring   measurements revealed that a preadsorbed BSM layer stabilizes IgG   through complexation on a polymeric model surface. Our findings link   well to the complex in vivo situation and suggest that functional   mucosal mimics can be created in situ for improved biomaterials   performance.
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  • Resultat 1-2 av 2
Typ av publikation
tidskriftsartikel (2)
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refereegranskat (2)
Författare/redaktör
Sandberg, Tomas (2)
Carlsson, Jan (1)
Feiler, Adam (1)
Blom, Hans (1)
Karlsson Ott, Marjam (1)
Lärosäte
Uppsala universitet (2)
Språk
Engelska (2)
Forskningsämne (UKÄ/SCB)
Naturvetenskap (2)
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