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- Sandberg, Tomas, et al.
(författare)
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Potential use of mucins as biomaterial coatings. I. Fractionation, characterization, and model adsorption of bovine, porcine, and human mucins
- 2009
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Ingår i: Journal of Biomedical Materials Research Part A. - : Wiley. - 1549-3296 .- 1552-4965. ; 91A:3, s. 762-772
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Tidskriftsartikel (refereegranskat)abstract
- Previously, we presented evidence that mucins have potential as biomaterial coatings. Here, we reveal substantial batch-to-batch variations for a frequently used commercial bovine salivary mucin preparation (BSM) and stress the importance of standardizing mucins intended for comparative purposes. "Mild" fractionation strategies, aiming at preserving natural mucin functions, were used to prepare two more defined BSM fractions as well as three mucin fractions from porcine gastric (PGM) and human salivary (MG1) sources. While the BSM and PGM were highly purified and mainly adopted random coil conformations in solution, the MG1 contained mucin-bound components (1.6 wt% albumin) and appeared compact. Average molar masses and root-mean-square radii for the predominant BSM, PGM, and MG1 species spanned 0.8-4.2 MDa and 46-86 nm, respectively. An ellipsometric evaluation, using hydrophilic and hydrophobic silica, showed the mucin adsorption to be slow and related to mucin charge, size, conformation, and compositional complexity. The mass uptakes on hydrophobic silica averaged 2.6, 2.6, and 5.0 mg/m(2), for BSM, PGM, and MG1, respectively. Finally, we find that stable mucin coatings can be formed on polymers of different wettability. The reported mucin preparations serve as platforms for a series of studies on the biocompatibility of mucin coatings.
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2. |
- Sandberg, Tomas, et al.
(författare)
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Potential use of mucins as biomaterial coatings. II. Mucin coatings affect the conformation and neutrophil-activating properties of adsorbed host proteins – Towards a mucosal mimic
- 2009
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Ingår i: Journal of Biomedical Materials Research: Part A. - : Wiley. - 1549-3296 .- 1552-4965. ; 91A:3, s. 773-785
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Tidskriftsartikel (refereegranskat)abstract
- In continuation of our recent fractionation and characterization study on mucins of bovine salivary (BSM), porcine gastric (PGM), and human salivary (MG1) origin, this study evaluates the effect of mucin precoating on the conformation and neutrophil-activating properties of host proteins adsorbed to a polyethylene terephthalate-based model biomaterial. Microscopy combined with assays for the neutrophil releases of reactive oxygen species and human neutrophil lipocalin showed that mucin precoating greatly reduced the strong immune-response normally induced by adsorbed immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA), respectively. A similar finding was made for the proinflammatory fibrinogen. Although the total uptakes of these proteins depended on the mucin surface concentration, a detailed composite analysis suggested the fraction Of surface-exposed protein to be a stronger determinant of coating performance. The unexpectedly low neutrophil activation showed by composites containing near-monolayer concentrations of exposed IgG and sIgA, respectively, suggested that these act synergistically with mucin on the surface. In support of this hypothesis, quartz crystal microbalance with dissipation monitoring measurements revealed that a preadsorbed BSM layer stabilizes IgG through complexation on a polymeric model surface. Our findings link well to the complex in vivo situation and suggest that functional mucosal mimics can be created in situ for improved biomaterials performance.
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