| 1. |
- Quaglia, Milena, et al.
(författare)
-
Better Measurement for Improved Diagnosis and Management of Alzheimer's Disease : Update on the Empir Neuromet Project
- 2018
-
Ingår i: Alzheimer's & Dementia. - : Wiley. - 1552-5260 .- 1552-5279. ; 14, s. P759-P760
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The development of novel therapies for Alzheimer’s Disease (AD) is constrained by the lack of available methods for preclinical diagnosis, despite extensive research on biomarker identification. Here, we present an update of progress from EMPIR NeuroMET, a project combining diverse expertise from five National Measurement Institutes (NMIs), with clinicians and academics, to overcome limitations in measurement methods in neurodegenerative disease diagnosis and treatment.
|
|
| 2. |
- , arseglia
(författare)
-
Erratum
- 2021
-
Ingår i: Alzheimer's & dementia : the journal of the Alzheimer's Association. - : Wiley. - 1552-5279. ; 17:1, s. 137-137
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
- Gatsinzi, Tom, et al.
(författare)
-
Localized caspase sensors for live cell imaging of amyloid-β induced apoptosis
- 2010
-
Ingår i: Alzheimer's & Dementia. - : Wiley. - 1552-5260 .- 1552-5279. ; 6:4, Supplement, s. S259-S260
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Background: Apoptosis is an evolutionary conserved cellular process important for normal development, maintenance of tissue homeostasis and an effective immune system. Cysteine-aspartic proteases, or caspases, are the major mediators of apoptosis, triggering processes which lead to cellular disruption. Dysregulation of apoptotic signaling has been shown to be involved in several pathological conditions, like cancer and degenerative disorders. Alzheimer's disease (AD) is the most common form of dementia involving massive cell death of neurons. However, the cause of AD at the present time is still unknown, although, amyloid-β (Aβ) peptide has been suggested to be the triggering factor. Methods:In order to detect localized caspase activation in live cells we designed sensors for caspase-3, -6 and -9 utilizing fluorescence resonance energy transfer (FRET). The FRET-ing sensor molecules, consisting of CFP and YFP separated by a linker containing a specific caspase cleavage motif, were designed to signal caspase cleavage by the loss of FRET. Differentiated SH-SY5Y cells were used as a model system for neurodegeneration. The cells were treated with oligomeric Aβ42 or staurosporine as a positive control of apoptosis. The cleavage of the sensors during induced apoptosis was verified by western blot analysis. Time-lapse FRET microscopy was used to monitor caspase activity in different parts of the cells. Results: In our study, when the cells were exposed to staurosporine we were able to detect local activity of caspase-6 initially in the soma of the cells, whereas caspase-6 activity in the neurites was delayed. Furthermore, our study shows that oligomeric Aβ42 is able to activate caspase-3, -6 and -9. In contrast to staurosporine, in Aβ42 treated cells loss of FRET occurred globally indicating that caspase was activated simultaneously in soma and axons. Conclusions: In conclusion, we show that our caspase-sensors are able to detect local caspase activity in vitro. We also show that exposure to oligomeric Aβ42 results in global activation of caspases in differentiated SH-SY5Y cells.
|
|
| 6. |
- Hagman, Göran, et al.
(författare)
-
Midlife hopelessness and white matter lesions two decades later : A population-based study
- 2010
-
Ingår i: Alzheimer's & Dementia. - : Elsevier. - 1552-5260 .- 1552-5279. ; 7:4, Supplement, s. 595-595
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Background: Hopelessness has been associated with increased cardiovas- cular disease mortality and morbidity, subclinical atherosclerosis and meta- bolic syndrome. This study investigates the relation between midlife hopelessness and white matter lesions (WMLs) 20 years later in a Finnish population of men and women. Methods: Participants of the Cardiovascular Risk Factors, Aging and Incidence of Dementia (CAIDE) study in Finland were derived from random, population-based samples previously surveyed in 1972,1977, 1982 or 1987. In 1998, 1449 (73%) individuals aged 65-79 years participated in the re-examination. A subgroup (n1⁄4112, including 39 dementia cases, 31 mild cognitive impairment (MCI) cases and 42 con- trols) underwent 1.5T MRI scanning at re-examination, and WMLs were as- sessed from FLAIR-images using a semi-quantitative visual rating scale. Hopelessness was measured by 2 questionnaire items (expectations about future and reaching goals). Results: Subjects with increased hopelessness had a significantly higher risk of developing more severe WMLs two de- cades later. OR (95% CI) was 4.35 (1.36-13.46) in ordinal regression anal- yses adjusted for age, sex education, follow-up time, presence of the APOEe4 allele, systolic blood pressure, BMI, history of stroke, heart infarct, smoking and level of midlife leisure physical activity. Conclusions: Higher levels of hopelessness at midlife seem to be related to more severe WMLs later in life. Since WMLs may contribute to late-life cognitive impairment, lifestyle management of midlife vascular risk factors (which also increase the risk of dementia and cognitive impairment) may have better effects if people’s expectations are more thoroughly discussed.
|
|
| 7. |
- Håkansson, Krister
(författare)
-
O2-07-01: Unmarried Life: Paving the way for dementia?
- 2008
-
Ingår i: Alzheimer's & Dementia. - : Wiley. - 1552-5260 .- 1552-5279. ; 4:4, Supplement, s. T146-
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Background: Although social networks and activities have recently been suggested to protect against dementia, few long-term follow-up studies exist. The main purpose of our study was to evaluate whether midlife marital status is related to late-life cognitive function. Methods: Participants of the Cardiovascular Risk Factors, Aging, and Dementia (CAIDE) study were derived from random, population-based samples previously studied. After an average follow-up of 21 years, 1449 individuals (73%) aged 65 to 79 years were re-examined in 1998. At re-examination 139 persons were diagnosed with some form of cognitive impairment: 82 of those with mild cognitive impairment (MCI) and 48 with Alzheimer's disease (AD)). The relation between midlife marital status and cognitive impairment was analyzed with adjustments for a number of other midlife factors, including education, BMI, cholesterol, blood pressure, occupation, physical activity, smoking habits and depression. Adjustments were also made for ApoE status, age at follow-up and gender. Results: Persons living with a partner in midlife were significantly less likely to show cognitive impairment compared to all other categories (single, separated or widowed). The highest risk increase was found for those widowed at midlife and still so at the follow-up (N=105). For Alzheimers disease specifically, the risk increase was almost eight-fold for this group compared to those married both at midlife and still so at late-life. Progressive adjustments for possible confounders did not weaken the associations. Conclusions: Living in a partner relation may imply cognitive and social challenges with a protective effect against cognitive impairment. Involvement of other factors is however suggested by the specific risk increase for widowed in relation to singles. Possible selection bias behind the strongly increased cognitive impairment risk for those widowed at midlife, in relation to the married group, seems unlikely. The long-term prospective design should also preclude any reverse causation effects behind the results.
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
- Mok, VCT, et al.
(författare)
-
Erratum
- 2021
-
Ingår i: Alzheimer's & dementia : the journal of the Alzheimer's Association. - : Wiley. - 1552-5279. ; 17:5, s. 906-907
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
|
|
