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Träfflista för sökning "L773:2045 2322 srt2:(2011)"

Sökning: L773:2045 2322 > (2011)

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1.
  • Kuzmenko, Anton, et al. (författare)
  • Single molecule tracking fluorescence microscopy in mitochondria reveals highly dynamic but confined movement of Tom40
  • 2011
  • Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 1, s. 195-
  • Tidskriftsartikel (refereegranskat)abstract
    • Tom40 is an integral protein of the mitochondrial outer membrane, which as the central component of the Translocase of the Outer Membrane (TOM) complex forms a channel for protein import. We characterize the diffusion properties of individual Tom40 molecules fused to the photoconvertable fluorescent protein Dendra2 with millisecond temporal resolution. By imaging individual Tom40 molecules in intact isolated yeast mitochondria using photoactivated localization microscopy with sub-diffraction limited spatial precision, we demonstrate that Tom40 movement in the outer mitochondrial membrane is highly dynamic but confined in nature, suggesting anchoring of the TOM complex as a whole.
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2.
  • Sandberg, Julia, et al. (författare)
  • Rapid flow-sorting to simultaneously resolve multiplex massively parallel sequencing products
  • 2011
  • Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 1:108
  • Tidskriftsartikel (refereegranskat)abstract
    • Sample preparation for Roche/454, ABI/SOLiD and Life Technologies/Ion Torrent sequencing are based on amplification of library fragments on the surface of beads prior to sequencing. Commonly, libraries are barcoded and pooled, to maximise the sequence output of each sequence run. Here, we describe a novel approach for normalization of multiplex next generation sequencing libraries after emulsion PCR. Briefly, amplified libraries carrying unique barcodes are prepared by fluorescent tagging of complementary sequences and then resolved by high-speed flow cytometric sorting of labeled emulsion PCR beads. The protocol is simple and provides an even sequence distribution of multiplex libraries when sequencing the flow-sorted beads. Moreover, since many empty and mixed emulsion PCR beads are removed, the approach gives rise to a substantial increase in sequence quality and mean read length, as compared to that obtained by standard enrichment protocols.
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