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Träfflista för sökning "LAR1:mau ;pers:(Cardenas Marite)"

Sökning: LAR1:mau > Cardenas Marite

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1.
  • Bertram, Nicolas, et al. (författare)
  • Nanodisc films for membrane protein studies by neutron reflection : Effect of the protein scaffold choice
  • 2015
  • Ingår i: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 31:30, s. 8386-8391
  • Tidskriftsartikel (refereegranskat)abstract
    • Nanodisc films are a promising approach to study the equilibrium conformation of membrane bound proteins in native-like environment. Here we compare nanodisc formation for NADPH-dependent cytochrome P450 oxidoreductase (POR) using two different scaffold proteins, MSP1D1 and MSP1E3D1. Despite the increased stability of POR loaded MSP1E3D1 based nanodiscs in comparison to MSP1D1 based nanodiscs, neutron reflection at the silicon–solution interface showed that POR loaded MSP1E3D1 based nanodisc films had poor surface coverage. This was the case, even when incubation was carried out under conditions that typically gave high coverage for empty nanodiscs. The low surface coverage affects the embedded POR coverage in the nanodisc film and limits the structural information that can be extracted from membrane bound proteins within them. Thus, nanodisc reconstitution on the smaller scaffold proteins is necessary for structural studies of membrane bound proteins in nanodisc films.
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2.
  • Brennich, Martha, et al. (författare)
  • Nanoparticle Characterization Methods : Applications of Synchrotron and Neutron Radiation
  • 2016
  • Ingår i: Pharmaceutical Nanotechnology. - Weinheim, Germany : John Wiley & Sons. - 9783527340545 - 9783527800681 ; , s. 157-174
  • Bokkapitel (refereegranskat)abstract
    • The characterization of materials at the atomic-, nano-, and microscales is of crucial importance in understanding and then tailoring their macroscale properties and function for end-use applications and for effective modern cradle-to-reuse materials cycling. Synchrotron light, as well as the complementary neutron beams, offer exquisite microscopy probes to look into the heart of materials. This chapter presents some examples of pharma-oriented nanoparticle characterization highlighting the possibilities of synchrotron light and neutron beams. Small-angle X-ray scattering (SAXS) is a well-established technique to probe nanoscale structures. SAXS can also deliver valuable information on the structure of self-assembled nanovectors, such as liposomes, which are recognized as efficient platforms for drug delivery. Future developments for neutron characterization will be driven in parallel with instrumental developments at existing sources and future facilities such as the European Spallation Source (ESS) being built in Sweden.  
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3.
  • Browning, Kathryn L., et al. (författare)
  • Effect of bilayer charge on lipoprotein lipid exchange
  • 2018
  • Ingår i: Colloids and Surfaces B. - : ELSEVIER SCIENCE BV. - 0927-7765 .- 1873-4367. ; 168, s. 117-125
  • Tidskriftsartikel (refereegranskat)abstract
    • Lipoproteins play a key role in the onset and development of atherosclerosis, the formation of lipid plaques at blood vessel walls. The plaque formation, as well as subsequent calcification, involves not only endothelial cells but also connective tissue, and is closely related to a wide range of cardiovascular syndromes, that together constitute the number one cause of death in the Western World. High (HDL) and low (LDL) density lipoproteins are of particular interest in relation to atherosclerosis, due to their protective and harmful effects, respectively. In an effort to elucidate the molecular mechanisms underlying this, and to identify factors determining lipid deposition and exchange at lipid membranes, we here employ neutron reflection (NR) and quartz crystal microbalance with dissipation (QCM-D) to study the effect of membrane charge on lipoprotein deposition and lipid exchange. Dimyristoylphosphatidylcholine (DMPC) bilayers containing varying amounts of negatively charged dimyristoylphosphatidylserine (DMPS) were used to vary membrane charge. It was found that the amount of hydrogenous material deposited from either HDL or LDL to the bilayer depends only weakly on membrane charge density. In contrast, increasing membrane charge resulted in an increase in the amount of lipids removed from the supported lipid bilayer, an effect particularly pronounced for LDL. The latter effects are in line with previously reported observations on atherosclerotic plaque prone regions of long-term hyperlipidaemia and type 2 diabetic patients, and may also provide some molecular clues into the relation between oxidative stress and atherosclerosis. 
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5.
  • Browning, T. K., et al. (författare)
  • Human lipoproteins at model cell membranes : Role of the lipoprotein class on lipid dynamics
  • 2017
  • Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 7
  • Tidskriftsartikel (refereegranskat)abstract
    • High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the ‘good’ cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the ‘bad’ cholesterol, depositing lipid material into the vascular wall.
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6.
  • Cárdenas, Marité, et al. (författare)
  • Cellulose Nanofibers for Biomedical Applications
  • 2016
  • Ingår i: Biopolymers for Medical Applications. - Taylor & Francis Group, 6000 Broken Sound Parkway NW, Suite 300, Boca Raton, FL 33487-2742 : CRC Press. - 9781498744966 ; , s. 213-232
  • Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
    • The creation of materials exploiting molecules from renewable resources and green processing routes in order to minimize contamination of the environment with toxic solvents and starting components, is an important step towards a sustainable society, and sustainable development is critical in all technological fields including biomedical engineering. In this context, the polysaccharides are an important family of molecules, as they can be derived from numerous natural sources including plants, bacteria, insects, animals, and also from by-products/waste-materials obtained from agricultural or fishery activities. Also, polysaccharides are biodegradable and can be broken down by common microorganisms found on land or in water. In nature, polysaccharides can be composed of one type of repeating unit (homopolysaccharides; starch and cellulose) or two or more types of repeating monomer (heteropolysaccharides; pectin, alginate). But despite using only a few basic building blocks, many unique and complex molecular structures with specific features and functions are assembled giving rise to a plethora of diverse carbohydrates. Some of the polysaccharides are classified as polyelectrolytes, and these are either negatively or positively charged. The intrinsic properties of such ionic polysaccharides are used in material science to produce stimuli-responsive materials where external stimuli (pH, ionic strength, and temperature) trigger, for example, a mechanical response in the material or a swelling mechanism that can be exploited in drug delivery. In addition to conventional polysaccharides, advanced genetic engineering also opens up the possibility for new, innovative, and structurally designed macromolecules with specific chemical and physical properties, allowing for better material structure-property control. Natural polysaccharides are typically biocompatible, possibly bioadhesive, and generally recognized as safe (GRAS) and, in conclusion, they are attractive materials to use in biomedical applications.
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7.
  • Cárdenas, Marité, et al. (författare)
  • Human Saliva Forms a Complex Film Structure on Alumina Surfaces
  • 2007
  • Ingår i: Biomacromolecules. - : American Chemical Society (ACS). - 1525-7797 .- 1526-4602. ; 8:1, s. 65-69
  • Tidskriftsartikel (refereegranskat)abstract
    • Films formed from saliva on surfaces are important for the maintenance of oral health and integrity by protection against chemical and/or biological agents. The aim of the present study was to investigate adsorbed amounts, thickness, and structure of films formed from human whole saliva on alumina surfaces by means of in situ ellipsometry, neutron reflectivity, and atomic force microscopy. Alumina (Al2O3, synthetic sapphire) is a relevant and interesting substrate for saliva adsorption studies as it has an isoelectric point close to that of tooth enamel. The results showed that saliva adsorbs rapidly on alumina. The film could be modeled in two layers:  an inner and dense thin region that forms a uniform layer and an outer, more diffuse and thicker region that protrudes toward the bulk of the solution. The film morphology described a uniformly covering dense layer and a second outer layer containing polydisperse adsorbed macromolecules or aggregates.
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8.
  • Cárdenas, Marité, et al. (författare)
  • Review of structural design guiding the development of lipid nanoparticles for nucleic acid delivery
  • 2023
  • Ingår i: Current Opinion in Colloid and Interface Science. - : Elsevier. - 1359-0294 .- 1879-0399. ; 66
  • Forskningsöversikt (refereegranskat)abstract
    • Lipid nanoparticles (LNPs) are the most versatile and successful gene delivery systems, notably highlighted by their use in vaccines against COVID-19. LNPs have a well-defined core–shell structure, each region with its own distinctive compositions, suited for a wide range of in vivo delivery applications. Here, we discuss how a detailed knowledge of LNP structure can guide LNP formulation to improve the efficiency of delivery of their nucleic acid payload. Perspectives are detailed on how LNP structural design can guide more efficient nucleic acid transfection. Views on key physical characterization techniques needed for such developments are outlined including opinions on biophysical approaches both correlating structure with functionality in biological fluids and improving their ability to escape the endosome and deliver they payload.
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9.
  • Cárdenas, Marité, et al. (författare)
  • Salivary mucin MUC5B could be an important component of in vitro pellicles of human saliva: An in situ ellipsometry and atomic force microscopy study
  • 2007
  • Ingår i: Biomacromolecules. - : American Chemical Society (ACS). - 1525-7797 .- 1526-4602. ; 8:4, s. 1149-1156
  • Tidskriftsartikel (refereegranskat)abstract
    • This paper describes a combined investigation of the salivary and MUC5B films structure and topography in conditions similar to those found in the oral cavity in terms of ionic strength, pH, and protein concentration. AFM and ellipsometry were successfully used to give a detailed picture of the film structure and topography both on hydrophilic and on hydrophobic substrata. Regardless of the substrata, the salivary film can be described as having a two sublayer structure in which an inner dense layer is decorated by large aggregates. However, the shape and height of these larger aggregates largely depend on the type of substrata used. Additionally, we show that the adsorption of MUC5B is controlled by the type of substrata and the MUC5B film topography is similar to that of the larger aggregates present in the salivary films, especially on hydrophobic substrates. Therefore, we conclude that MUC5B is a major component in the salivary film when formed on hydrophobic substrates. Furthermore, we studied how resistant the salivary and MUC5B films are against elutability by buffer rinsing and addition of SDS solution. We conclude that the adsorbed proteins contain fractions with varying binding strengths to the two types of surfaces. Specifically, we have shown that the large MUC5B biomacromolecules on the hydrophobic substrates are especially resistant to both elution with buffer solution and SDS. Therefore, these large mucins can be responsible for the increased resistance of HWS films on hydrophobic substrates and can protect the intraoral surfaces against surface-active components present in oral health care products.
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10.
  • Cárdenas, Marité, et al. (författare)
  • Solubilization of sphingomyelin vesicles by addition of a bile salt.
  • 2008
  • Ingår i: Chemistry and Physics of Lipids. - : Elsevier BV. - 0009-3084 .- 1873-2941. ; 151:Sep 25, s. 10-17
  • Tidskriftsartikel (refereegranskat)abstract
    • The interactions of the bile salt sodium taurocholate (TC) in 50mM Trizma-HCl buffer and 150mM NaCl (pH 9) at 37 degrees C with membranes composed of sphingomyelin (SM) were studied by dynamic light scattering, cryogenic transmission electron microscopy (cryo-TEM) and turbidity measurements. Small unilamellar SM vesicles were prepared by extrusion. Below the CMC of TC, taurocholate addition leads to vesicle growth due to incorporation of the taurocholate molecules into the vesicle bilayer. At around half the CMC of the bile salt, the SM vesicles are transformed into SM/TC mixed worm-like micelles, which are visualized by cryo-TEM for the first time. Further increase in the taurocholate concentration leads to the rupture of these structures into small spherical micelles. Interestingly, large non-spherical micelles were also identified for pure taurocholate solutions. Similar threadlike structures have been reported earlier for the bile salt sodium taurodeoxycholate [Rich, A., Blow, D., 1958. Nature 182, 1777; Blow, D.M., Rich, A., 1960. J. Am. Chem. Soc. 82, 3566-3571; Galantini, L., Giglio, E., La Mesa, C., Viorel-Pavel, N., Punzo, F., 2002. Langmuir 18, 2812] and for mixtures of taurocholate and phosphatidylcholate [Ulmius, J., Lindblom, G., Wennerström, H., Johansson, L.B.-A., Fontel, K., Söderman, O., Ardvisson, G., 1982. Biochemistry 21, 1553; Hjelm, R.P., Thiyagarajan, P., Alkan-Onyuksel, H., 1992. J. Phys. Chem. 96, 8653] as determined by various scattering methods.
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