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Search: WFRF:(Bauer David) > Malmö University

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1.
  • Sulc, Robert, et al. (author)
  • Phospholipid imprinted polymers as selective endotoxin scavengers
  • 2017
  • In: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 7
  • Journal article (peer-reviewed)abstract
    • Herein we explore phospholipid imprinting as a means to design receptors for complex glycolipids comprising the toxic lipopolysaccharide endotoxin. A series of polymerizable bis-imidazolium and urea hosts were evaluated as cationic and neutral hosts for phosphates and phosphonates, the latter used as mimics of the phospholipid head groups. The bis-imidazolium hosts interacted with the guests in a cooperative manner leading to the presence of tight and well defined 1:2 ternary complexes. Optimized monomer combinations were subsequently used for imprinting of phosphatidic acid as an endotoxin dummy template. Presence of the aforementioned ternary complexes during polymerization resulted in imprinting of lipid dimers - the latter believed to crudely mimic the endotoxin Lipid A motif. The polymers were characterized with respect to template rebinding, binding affinity, capacity and common structural properties, leading to the identification of polymers which were thereafter subjected to an industrially validated endotoxin removal test. Two of the polymers were capable of removing endotoxin down to levels well below the accepted threshold (0.005 EU/mg API) in pharmaceutical production.
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2.
  • Wierzbicka, Celina, et al. (author)
  • Cationic pTyr/pSer imprinted polymers based on a bis-imidazolium host monomer : phosphopeptide recognition in aqueous buffers demonstrated by mu-liquid chromatography and monolithic columns
  • 2017
  • In: Journal of materials chemistry. B. - : Royal Society of Chemistry. - 2050-750X .- 2050-7518. ; 5:5, s. 953-960
  • Journal article (peer-reviewed)abstract
    • We report on the design and characterization of imprinted cationic host polymers for selective trapping of phosphoserine and phosphotyrosine peptides. A series of imidazolium host monomers were synthesized and characterized with respect to binding affinity and stoichiometry of interaction with salts of phenylphosphonic acid. The strongest binders were subsequently used for the preparation of imprinted polymers in the form of crushed monoliths, using Fmoc-phosphotyrosine-ethyl ester or Fmoc-phosphoserine-ethyl ester as templates in combination with a hydrophilic crosslinking monomer. The polymers were compared with respect to binding and its dependence on solvent, and whether charged or uncharged host monomers were used. The recipes were subsequently implemented in the capillary monolith format for evaluation by micro-liquid chromatography in both buffered and organic media. Results from both tested formats reveal that the cationic host polymers displayed enhanced recognition in polar and buffered media, in contrast to neutral urea-based hosts which showed best results in acetonitrile rich mobile phases.
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  • Result 1-2 of 2
Type of publication
journal article (2)
Type of content
peer-reviewed (2)
Author/Editor
Sellergren, Börje (2)
Wierzbicka, Celina (2)
Bauer, David (2)
Shinde, Sudhirkumar (1)
Irgum, Knut (1)
Vilela, Filipe (1)
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Liu, Mingquan (1)
Sulc, Robert (1)
Szekely, Gyorgy (1)
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University
Malmö University  (2)Undo refine
Umeå University (1)
Language
English (2)
Research subject (UKÄ/SCB)
Natural sciences (1)
Year
2017 (2)

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