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Diagnostic challeng...
Diagnostic challenges for the detection of emerging pathogens : A case study involving the incursion of Pseudomonas syringae pv. actinidiae in New Zealand
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- Taylor, Robert K. (author)
- Ministry for Primary Industries, New Zealand
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- Chapman, Joanne R. (author)
- Linnéuniversitetet,Institutionen för biologi och miljö (BOM),Ministry for Primary Industries, New Zealand
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- Romberg, Megan K. (author)
- Ministry for Primary Industries, New Zealand;USDA-APHIS, USA
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- Weir, Bevan S. (author)
- Landcare Research, New Zealand
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- Vanneste, Joel L. (author)
- The New Zealand Institute for Plant and Food Research Ltd., New Zealand
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- Everett, Kerry R. (author)
- The New Zealand Institute for Plant and Food Research Ltd., New Zealand
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- Ward, Lisa I. (author)
- Ministry for Primary Industries, New Zealand
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- Liefting, Lia W. (author)
- Ministry for Primary Industries, New Zealand
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- Lebas, Benedicte S. M. (author)
- Ministry for Primary Industries, New Zealand
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- Alexander, Brett J. R. (author)
- Ministry for Primary Industries, New Zealand
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(creator_code:org_t)
- 2014-08-19
- 2014
- English.
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In: Detection and Diagnostics of Plant Pathogens. - Dordrecht : Springer. - 9789401790208 - 9789401790192 ; , s. 71-86
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- In November 2010, Pseudomonas syringae pv. actinidiae (Psa) was detected for the first time in New Zealand. This finding triggered one of the largest surveillance and diagnostic programmes seen in New Zealandâs horticultural industry. During this response, over 912 kiwifruit orchards and 14,500 samples were screened and tested for the presence of Psa. The initial objectives of the response were to confirm the causal agent, determine disease prevalence and identify possible mechanisms of spread with the aim of identifying management options to contain the outbreak. Molecular diagnoses and characterisation of the Psa strains isolated during the response was conducted using a range of techniques that included qPCR, rep-PCR fingerprinting, multilocus sequence analysis, and next generation sequencing. The usefulness and challenges of using the molecular techniques available at the time for Psa detection and characterisation during the response are discussed. © Springer Science+Business Media Dordrecht 2014.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Bacteria; Molecular biology; Plasma diagnostics; Polymerase chain reaction; Space surveillance
- Actinidia spp; Horticultural industry; Molecular techniques; Multilocus sequence analysis; New zealand; Next-generation sequencing; Pseudomonas syringae; Response
- Diagnosis
- Mikrobiologi
- Microbiology
Publication and Content Type
- vet (subject category)
- kap (subject category)
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- By the author/editor
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Taylor, Robert K ...
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Chapman, Joanne ...
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Romberg, Megan K ...
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Weir, Bevan S.
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Vanneste, Joel L ...
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Everett, Kerry R ...
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Ward, Lisa I.
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Liefting, Lia W.
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Lebas, Benedicte ...
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Alexander, Brett ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Detection and Di ...
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Linnaeus University