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Miniaturized and multiplexed high-content screening of drug and immune sensitivity in a multichambered microwell chip

Sandström, Niklas, 1981- (author)
KTH,Biofysik,Science for Life Laboratory, SciLifeLab
Carannante, Valentina (author)
KTH,Biofysik,Science for Life Laboratory, SciLifeLab,Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Sci Life Lab, S-17165 Solna, Sweden.
Olofsson, Karl (author)
KTH,Science for Life Laboratory, SciLifeLab,Biofysik
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Sandoz, Patrick (author)
KTH,Biofysik,Science for Life Laboratory, SciLifeLab
Moussaud-Lamodiere, Elisabeth L. (author)
Karolinska Institutet
Seashore-Ludlow, Brinton (author)
Karolinska Institutet
van Ooijen, Hanna (author)
KTH,Biofysik,Science for Life Laboratory, SciLifeLab
Verron, Quentin (author)
KTH,Biofysik,Science for Life Laboratory, SciLifeLab
Frisk, Thomas (author)
KTH,Biomedicinsk fysik och röntgenfysik,Science for Life Laboratory, SciLifeLab
Takai, Madoka (author)
Univ Tokyo, Dept Bioengn, 7-3-1 Hongo,Bunkyo-Ku, Tokyo 1138656, Japan.
Wiklund, Martin (author)
KTH,Science for Life Laboratory, SciLifeLab,Biofysik
Ostling, Paeivi (author)
Karolinska Institutet
Önfelt, Björn (author)
KTH,Science for Life Laboratory, SciLifeLab,Biofysik,Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Sci Life Lab, S-17165 Solna, Sweden.
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 (creator_code:org_t)
Elsevier BV, 2022
2022
English.
In: CELL REPORTS METHODS. - : Elsevier BV. - 2667-2375. ; 2:7
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Here, we present a methodology based on multiplexed fluorescence screening of two-or three-dimensional cell cultures in a newly designed multichambered microwell chip, allowing direct assessment of drug or im-mune cell cytotoxic efficacy. We establish a framework for cell culture, formation of tumor spheroids, fluores-cence labeling, and imaging of fixed or live cells at various magnifications directly in the chip together with data analysis and interpretation. The methodology is demonstrated by drug cytotoxicity screening using ovarian and non-small cell lung cancer cells and by cellular cytotoxicity screening targeting tumor spheroids of renal carcinoma and ovarian carcinoma with natural killer cells from healthy donors. The miniaturized format allowing long-term cell culture, efficient screening, and high-quality imaging of small sample volumes makes this methodology promising for individualized cytotoxicity tests for precision medicine.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

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