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- Russom, Aman, et al.
(författare)
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Ultra-rapid melting curve analysis on beads for high-throughput genotyping of single nucleotide polymorphism
- 2005
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Ingår i: Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference. - : Transducers Research Foundations. - 9780974361116 ; , s. 1006-1008
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Konferensbidrag (refereegranskat)abstract
- This report describes a rapid solid-phase melting curve analysis method based on dynamic allele-specific hybridization (DASH) for single nucleotide polymorphism (SNP) genotyping. Beads with DNA duplexes are immobilized on the surface of a microheater chip. SNP on PCR products were scored, illustrating the sensitivity and robustness of the system. Single-bead detection and multiplexing were performed with a heating rate more than 20 times faster than conventional DASH. Hence, analyses that took more than 15 minutes could be performed in less than 1 minute, enabling ultra-rapid SNP analysis.
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