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- Liu, Johan, 1960, et al.
(författare)
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Stem Cell Growth and Migration on Nanofibrous Polymer Scaffolds and Micro-Fluidic Channels on Silicon-Chip
- 2009
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Ingår i: Proceedings of the 2009 Electronic Components and Technology Conference. - 0569-5503. - 9781424444762 ; , s. 1080-1085
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Konferensbidrag (refereegranskat)abstract
- Stem cell growth and migration on nanofibrous scaffolds and micro-fluidic channels on Silicon-Chip were studied by using neural stem cells isolated from adult rats' brain. Electrospinning and lithographic technique were used for developing nanofibrous-polylactic acid (PLA) and polyurethane (PU) based-scaffolds and micro-fluidic channels on Si-Chips respectively. Immunocytochemical and morphological analysis showed better cell-matrix interaction with profound adhesion, proliferation and migration on the developed scaffolds. Cell culture assay with microfluidic channel revealed the ability of developed channel system in guiding neuronal stem cell growth towards specified directions. These studies extend the possibility of using developed nanofibrous scaffolds and micro-fluidic channel system for future electrical signal transmission based on living neural stem cells.
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| 2. |
- Carlberg, Björn, 1983, et al.
(författare)
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Electrospun polyurethane scaffolds for proliferation and neuronal differentiation of human embryonic stem cells.
- 2009
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Ingår i: Biomedical materials (Bristol, England). - : IOP Publishing. - 1748-605X .- 1748-6041. ; 4:4
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Tidskriftsartikel (refereegranskat)abstract
- Adult central nervous system (CNS) tissue has a limited capacity to recover after trauma or disease. Hence, tissue engineering scaffolds intended for CNS repair and rehabilitation have been subject to intense research effort. Electrospun porous scaffolds, mimicking the natural three-dimensional environment of the in vivo extracellular matrix (ECM) and providing physical support, have been identified as promising candidates for CNS tissue engineering. The present study demonstrates in vitro culturing and neuronal differentiation of human embryonic stem cells (hESCs) on electrospun fibrous polyurethane scaffolds. Electrospun scaffolds composed of biocompatible polyurethane resin (Desmopan 9370A, Bayer MaterialScience AG) were prepared with a vertical electrospinning setup. Resulting scaffolds, with a thickness of approximately 150 microm, exhibited high porosity (84%) and a bimodal pore size distribution with peaks at 5-6 and 1 microm. The mean fiber diameter was measured to approximately 360 nm with a standard deviation of 80 nm. The undifferentiated hESC line SA002 (Cellartis AB, Göteborg, Sweden) was seeded and cultured on the produced scaffolds and allowed propagation and then differentiation for up to 47 days. Cultivation of hESC on electrospun fibrous scaffolds proved successful and neuronal differentiation was observed via standard immunocytochemistry. The results indicate that predominantly dopaminergic tyrosine hydroxylase (TH) positive neurons are derived in co-culture with fibrous scaffolds, in comparison to reference cultures under the same differentiation conditions displaying large proportions of GFAP positive cell types. Scanning electron micrographs confirm neurite outgrowth and connection to adjacent cells, as well as cell attachment to individual fibers of the fibrous scaffold. Consequently, electrospun polyurethane scaffolds have been proven feasible as a substrate for hESC propagation and neuronal differentiation. The physical interaction between cells and the fibrous scaffold indicates that these scaffolds provide a three-dimensional physical structure; a potential candidate for neural tissue engineering repair and rehabilitation.
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| 3. |
- Carlberg, Björn, 1983
(författare)
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Integration of Electrospun Materials in Microelectronic and Biomedical Applications
- 2009
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis demonstrates the use of electrospinning and the novel properties of electrospunmaterials in two fields.The microelectronics industry has identified thermal interface materials as one of themajor bottlenecks hindering further integration at packaging level. New concepts basedon metal-polymer composite architectures are needed to fulfill current and future thermaland thermomechanical requirements on thermal interface materials at maintained costefficiency. In this thesis, an interpenetrating phase polymer-metal composite for thermalinterface material applications has been developed and characterized. Both fabricationand metrology equipment has been developed for the purpose at hand. The composite isbased on a porous electrospun polymer carrier infiltrated with a high thermal conductivitymetal phase. The two phases form two fully interpenetrating networks in the composite.Efficient heat transfer is achieved through the continuous metal phase, while the polymericphase defines geometry and phase composition. The devised composite architecture is believedto be a step towards meeting current and future demands on thermal performanceand thermomechanical reliability in microelectronic products.Furthermore, the thesis presents initial results of human embryonic stem cell proliferationand neural differentiation in co-culture with electrospun scaffolds, of interestin future regenerative medicine based on stem cells. Results indicate that physical cuesemanating from cell-scaffold interactions affect cells towards a neuronal fate during differentiation,a phenomenon consistent with reports in literature on physical cues influencingstem cell fate. To allow for deeper analysis on cell-scaffold interactions of thetype described above, a microfabricated platform was developed for the purpose. A novelmethod for direct photolithographic micropatterning of electrospun polyurethane fibrousfilms over large surfaces has been devised. The method allows for assembly of complexelectrospun microstructures on single substrates via a multilayer approach involving multiplephotolithographic exposures, analogous to conventional photolithography in microfabricationof solid state devices. Indeed, this technique can find application in a variety ofapplications where it is beneficial to integrate micropatterned electrospun structures intomicrofabricated devices, in particular within biomedical engineering applications.
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