| 1. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 2. |
- Fenstermacher, M.E., et al.
(författare)
-
DIII-D research advancing the physics basis for optimizing the tokamak approach to fusion energy
- 2022
-
Ingår i: Nuclear Fusion. - : IOP Publishing. - 0029-5515 .- 1741-4326. ; 62:4
-
Tidskriftsartikel (refereegranskat)abstract
- DIII-D physics research addresses critical challenges for the operation of ITER and the next generation of fusion energy devices. This is done through a focus on innovations to provide solutions for high performance long pulse operation, coupled with fundamental plasma physics understanding and model validation, to drive scenario development by integrating high performance core and boundary plasmas. Substantial increases in off-axis current drive efficiency from an innovative top launch system for EC power, and in pressure broadening for Alfven eigenmode control from a co-/counter-I p steerable off-axis neutral beam, all improve the prospects for optimization of future long pulse/steady state high performance tokamak operation. Fundamental studies into the modes that drive the evolution of the pedestal pressure profile and electron vs ion heat flux validate predictive models of pedestal recovery after ELMs. Understanding the physics mechanisms of ELM control and density pumpout by 3D magnetic perturbation fields leads to confident predictions for ITER and future devices. Validated modeling of high-Z shattered pellet injection for disruption mitigation, runaway electron dissipation, and techniques for disruption prediction and avoidance including machine learning, give confidence in handling disruptivity for future devices. For the non-nuclear phase of ITER, two actuators are identified to lower the L-H threshold power in hydrogen plasmas. With this physics understanding and suite of capabilities, a high poloidal beta optimized-core scenario with an internal transport barrier that projects nearly to Q = 10 in ITER at ∼8 MA was coupled to a detached divertor, and a near super H-mode optimized-pedestal scenario with co-I p beam injection was coupled to a radiative divertor. The hybrid core scenario was achieved directly, without the need for anomalous current diffusion, using off-axis current drive actuators. Also, a controller to assess proximity to stability limits and regulate β N in the ITER baseline scenario, based on plasma response to probing 3D fields, was demonstrated. Finally, innovative tokamak operation using a negative triangularity shape showed many attractive features for future pilot plant operation.
|
|
| 3. |
- Aad, G., et al.
(författare)
-
- 2013
-
Ingår i: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :3
-
Tidskriftsartikel (refereegranskat)
|
|
| 4. |
- Chen, Zhen, et al.
(författare)
-
Multifunctional conductive hydrogels and their applications as smart wearable devices
- 2021
-
Ingår i: Journal of materials chemistry. B. - : Royal Society of Chemistry (RSC). - 2050-750X .- 2050-7518. ; 9:11, s. 2561-2583
-
Forskningsöversikt (refereegranskat)abstract
- Recently, hydrogekbased conductive materials and their applications as smart wearable devices have been paid tremendous attention due to their high stretchability, flexibility, and excellent biocompatibility. Compared with single functional conductive hydrogels, multifunctional conductive hydrogels are more advantageous to match various demands for practical applications. This review focuses on multifunctional conductive hydrogels applied for smart wearable devices. Representative strategies for conduction of hydrogels are discussed firstly: (1) electronic conduction based on the conductive fillers and (2) ionic conduction based on charged ions. Then, the common and intensive research on multiple functionahties of conductive hydrogels, such as mechanical properties, conductive and sensory properties, anti-freezing and moisturizing properties, and adhesion and self-healing properties is presented. The applications of multifunctional conductive hydrogels such as in human motion sensors, sensory skins, and personal healthcare diagnosis are provided in the third part. Finally, we offer our perspective on open challenges and future areas of interest for multifunctional conductive hydrogels used as smart wearable devices.
|
|
| 5. |
- Chen, Song, et al.
(författare)
-
Formation of Amorphous Iron-Calcium Phosphate with High Stability
- 2023
-
Ingår i: Advanced Materials. - : John Wiley & Sons. - 0935-9648 .- 1521-4095. ; 35:33
-
Tidskriftsartikel (refereegranskat)abstract
- Amorphous iron-calcium phosphate (Fe-ACP) plays a vital role in the mechanical properties of teeth of some rodents, which are very hard, but its formation process and synthetic route remain unknown. Here, the synthesis and characterization of an iron-bearing amorphous calcium phosphate in the presence of ammonium iron citrate (AIC) are reported. The iron is distributed homogeneously on the nanometer scale in the resulting particles. The prepared Fe-ACP particles can be highly stable in aqueous media, including water, simulated body fluid, and acetate buffer solution (pH 4). In vitro study demonstrates that these particles have good biocompatibility and osteogenic properties. Subsequently, Spark Plasma Sintering (SPS) is utilized to consolidate the initial Fe-ACP powders. The results show that the hardness of the ceramics increases with the increase of iron content, but an excess of iron leads to a rapid decline in hardness. Calcium iron phosphate ceramics with a hardness of 4 GPa can be achieved, which is higher than that of human enamel. Furthermore, the ceramics composed of iron-calcium phosphates show enhanced acid resistance. This study provides a novel route to prepare Fe-ACP, and presents the potential role of Fe-ACP in biomineralization and as starting material to fabricate acid-resistant high-performance bioceramics.
|
|
| 6. |
- Chen, XÍ, et al.
(författare)
-
TDHQ Enabling Fine-granularity Adaptive Loading for SSB-DMT Systems
- 2018
-
Ingår i: IEEE Photonics Technology Letters. - : Institute of Electrical and Electronics Engineers Inc.. - 1041-1135 .- 1941-0174. ; 30:19, s. 1687-1690
-
Tidskriftsartikel (refereegranskat)abstract
- In this letter, we introduce time domain hybrid quadrature amplitude modulation (TDHQ) for the single sideband (SSB) discrete multi-tone (DMT) systems. Experimental results reveal that with a single precoding set and the proposed adaptive loading algorithm, the TDHQ scheme can achieve finer granularity and therefore smoother continuous growth of data rate than that with the conventional quadrature amplitude modulation (QAM). Besides, thanks to the frame construction and the tailored mapping rule, the scheme with TDHQ has an obviously better peak to average power ratio (PAPR).
|
|
| 7. |
- Chen, Yuejun, et al.
(författare)
-
Flow Expansion and Deflection Downstream of a Symmetric Multi-gate Sluice Structure
- 2020
-
Ingår i: KSCE Journal of Civil Engineering. - : Springer. - 1226-7988 .- 1976-3808. ; 24:2, s. 471-482
-
Tidskriftsartikel (refereegranskat)abstract
- A sluice structure with multiple gates is often followed by an enlarging channel downstream. Experiments are conducted for different enlargement ratios in cross-section and Froude numbers at the gates. A large-scale PIV system is adopted to capture the surface flow field for examination of the flow features. The study shows that, despite the centrally placed sluice structure with symmetric outflow, a hydraulic jump occurs and the main flow downstream exhibits, in terms of expansion and deflection, a high degree of asymmetry and formation of large circulation zones. The degree of deflection increases significantly along the longitudinal direction for all the enlargement ratios except the smallest. The toe of the hydraulic jump is controlled at the upper edge of the sloping surface downstream of the gates. In light of outflow width, Froude number and enlargement ratio, the flow is classified into three regions. Immediately downstream, the effect of outflow width gradually decreases with increasing Froude number. Further downstream, the degree of deflection augments with increasing enlargement ratio, with circulation zones. At a given cross-section, the degree of expansion is positively related to the enlargement ratio. The study is expected to provide guidance for examination of similar issues of flow pattern and erosion protection design.
|
|
| 8. |
- Fu, Yuxiang, et al.
(författare)
-
Congestion-Aware Dynamic Elevator Assignment for Partially Connected 3D-NoCs
- 2019
-
Ingår i: 2019 IEEE INTERNATIONAL SYMPOSIUM ON CIRCUITS AND SYSTEMS (ISCAS). - : IEEE. - 9781728103976
-
Konferensbidrag (refereegranskat)abstract
- The combination of Network-on-Chips (NoCs) and 3D IC technology, 3D NoCs, has been proven to be able to achieve a great improvement in both network performance and power consumption compared to 2D NoCs. In the traditional 3D NoC, all routers are vertically connected. Due to the large overhead of Through-Silicon-Via (TSV, e.g., low fabrication yield and the occupied silicon area), the partially connected 3D NoC has emerged. The assignment method determines the traffic loads of the vertical links (elevators), thus has a great impact on 3D-NoCs' performance. In this paper, we propose a congestion-aware dynamic elevator assignment (CDA) scheme, which takes both the distance factors and network congestion information into account. Experiments show that the performance of the proposed CDA scheme is improved by 67% to 87% compared to the random selection scheme, 8% to 25% compared to SelByDis-1, and 13% to 18% compared to SelByDis-2.
|
|
| 9. |
|
|
| 10. |
- Baudry, Benoit, et al.
(författare)
-
A Software-Repair Robot Based on Continual Learning
- 2021
-
Ingår i: IEEE Software. - : Institute of Electrical and Electronics Engineers (IEEE). - 0740-7459 .- 1937-4194. ; 38:4, s. 28-35
-
Tidskriftsartikel (refereegranskat)abstract
- Software bugs are common, and correcting them accounts for a significant portion of the costs in the software development and maintenance process. In this article, we discuss R-Hero, our novel system for learning how to fix bugs based on continual training.
|
|
