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- 2019
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Tidskriftsartikel (refereegranskat)
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| 2. |
- Fornell, Anna, et al.
(författare)
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A microfluidic platform for SAXS measurements of liquid samples
- 2022
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Konferensbidrag (refereegranskat)abstract
- Small-angle X-ray scattering (SAXS) is a technique that can measure the size and shape of small particles such as proteins and nanoparticles using X-rays. At MAX IV, we are developing a microfluidic sample delivery platform to measure liquid samples containing proteins under flow using SAXS. One of the main advantages of using microfluidics is that the sample is continuously flowing, thus minimizing the risk of radiation damage as the sample is continuously refreshed. Other advantages include low sample volume and the possibility to study dynamic processes, e.g. mixing. To obtain good SAXS signals, the X-ray properties of the chip material are essential. The microfluidic chip must have low attenuation of X-rays, low background scattering, and high resistance to X-ray-induced damage, and preferably be low cost and easy to fabricate. In this work, we have evaluated the performance of two different polymer microfluidic chips for SAXS measurements.
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| 3. |
- Fornell, Anna, et al.
(författare)
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A Microfluidic Platform for Synchrotron X-ray Studies of Proteins
- 2021
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Konferensbidrag (refereegranskat)abstract
- New tools are needed to allow for complex protein dynamics studies, especially to study proteins in their native states. In the AdaptoCell project a microfluidic platform for academic and industrial users at MAX IV Laboratory is being developed. MAX IV is a Swedish national laboratory providing brilliant synchrotron X-rays for research. Due to the high photon flux, sensitive samples such as proteins are prone to rapid radiation damage; thus, it is advantageous to have the liquid sample underflow to refresh the sample continuously. This, in combination with small volumes, makes microfluidics a highly suitable sample environment for protein studies at MAX IV. The AdaptoCell platform is being integrated at three beamlines:Balder (X-ray absorption/emission spectroscopy), CoSAXS (small angle x-ray scattering) and Micromax (serial synchrotron crystallography). Currently, the platform is fully available atBalder, under commissioning at CoSAXS and being developed for MicroMAX.
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| 4. |
- Fornell, Anna, et al.
(författare)
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AdaptoCell : Microfluidics at MAX IV Laboratory
- 2022
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Ingår i: 25th Swedish Conference on Macromolecular Structure and Function.
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Konferensbidrag (refereegranskat)abstract
- The AdaptoCell project at MAX IV has developed a microfluidic sample delivery platform for academic and industrial users to enable studies of protein samples in solution and in microcrystals underflow. The platform is compatible with various X-ray techniques and has so far been integrated onto two beamlines at MAX IV: the CoSAXS beamline for small angle X-ray scattering studies and the Balder beamline for X-ray absorption spectroscopy studies. Initial implementation of the platform for serial crystallography sample delivery is ongoing and will be integrated onto the BioMAX and MicroMAX beamlines once commissioned. With this platform, we aim to meet the demand from our user community for studying proteins at physiologically relevant temperatures and give the ability to follow dynamical processes in situ as well as decreasing sample volumes and radiation damage.To determine the optimized flow rates and components for mixing etc. using different microfluidic chips, a dedicated off(beam)line test station with a microscope has been established at the Biolab. The Biolab also provides a number of characterization techniques, such as Dynamic Light Scattering, UV-Vis spectrophotometry, for quality control of the samples; as well as an anaerobic chamber for preparation and characterization of metalloproteins. The microfluidic flows are controlled via syringe pumps or a pressure-driven system. Channel design varies, depending on the needs of the experiment, from straight channel, cross-junction to herringbone micromixers etc. On-chip mixing of buffers with different viscosity, pH, ion strength and protein concentrations has been demonstrated successful and will be presented.
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| 5. |
- Fornell, Anna, et al.
(författare)
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AdaptoCell – Microfluidic Platforms at MAX IV Laboratory
- 2021
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Konferensbidrag (refereegranskat)abstract
- In the AdaptoCell project, we are developing microfluidic platforms for X-ray studies of liquid samples. Microfluidics is a suitable technology for samples that are prone to radiation damage, such as proteins. By having the sample underflow, the sample is continuously refreshed, and the risk of radiation damage is reduced. The technology is also suitable for investigating dynamic events such as in situ mixing. The microfluidic platforms are being integrated at three beamlines at MAX IV Laboratory: Balder (X-ray absorption/emission spectroscopy), CoSAXS (small angle x-ray scattering) and MicroMAX (serial synchrotron crystallography). Currently, the platforms are available for users at Balder and CoSAXS, which is under development at MicroMAX. In addition, we also provide a microfluidic offline test station where users can test their samples and optimise their devices before the beam time. The main components of the microfluidic setup are the pressure-driven flow controller and the microfluidic chip. We mainly use commercially available polymer microfluidic chips made of COC (cyclic olefin copolymer). COC is used as a chip material as it has high X-ray transmission and high resistance to radiation damage. There are several different chip designs available such as straight channel chips, droplet generator chips and mixing chips. We believe the AdaptoCell platforms will be useful and versatile sample environments for academic and industrial users at MAX IV Laboratory who want to perform experiments with liquid samples under flow.
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| 6. |
- Sequeira, Ana F., et al.
(författare)
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Cross-Eyed : Cross-Spectral Iris/Periocular Recognition Database and Competition
- 2016
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Ingår i: Proceedings of the 15th International Conference of the Biometrics Special Interest Group. - Piscataway, N.J. : IEEE. - 9783885796541 - 9781509007806
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Konferensbidrag (refereegranskat)abstract
- This work presents a novel dual-spectrum database containing both iris and periocular images synchronously captured from a distance and within a realistic indoor environment. This database was used in the 1st Cross-Spectrum Iris/Periocular Recognition Competition (Cross-Eyed 2016). This competition aimed at recording recent advances in cross- spectrum iris and periocular recognition. Six submissions were evaluated for cross-spectrum periocular recognition, and three for iris recognition. The submitted algorithms are briefly introduced. Detailed results are reported in this paper, and comparison of the results is discussed.
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| 7. |
- Sequeira, Ana F., et al.
(författare)
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Cross-Eyed 2017 : Cross-Spectral Iris/Periocular Recognition Competition
- 2017
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Konferensbidrag (refereegranskat)abstract
- This work presents the 2nd Cross-Spectrum Iris/Periocular Recognition Competition (Cross-Eyed2017). The main goal of the competition is to promote and evaluate advances in cross-spectrum iris and periocular recognition. This second edition registered an increase in the participation numbers ranging from academia to industry: five teams submitted twelve methods for the periocular task and five for the iris task. The benchmark dataset is an enlarged version of the dual-spectrum database containing both iris and periocular images synchronously captured from a distance and within a realistic indoor environment. The evaluation was performed on an undisclosed test-set. Methodology, tested algorithms, and obtained results are reported in this paper identifying the remaining challenges in path forward. © 2017 IEEE
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