| 1. |
- Collins, Matthew D, et al.
(författare)
-
Luteococcus sanguinis sp. nov., isolated from human blood.
- 2003
-
Ingår i: International journal of systematic and evolutionary microbiology. - 1466-5026. ; 53:Pt 6, s. 1889-91
-
Tidskriftsartikel (refereegranskat)abstract
- An unusual catalase-positive, Gram-positive, coccus-shaped bacterium that originated from a human blood specimen was subjected to a polyphasic taxonomic study. Cell-wall murein and lipid composition analyses indicated that the unknown isolate was a member of the genus Luteococcus. The results of comparative 16S rRNA gene sequence analysis were consistent with chemotaxonomic findings and showed that the unidentified bacterium represents a hitherto unknown sublineage within the genus Luteococcus that is closely related to, but distinct from, Luteococcus japonicus. On the basis of both phenotypic and phylogenetic evidence, it is proposed that the unknown bacterium from human blood should be classified as Luteococcus sanguinis sp. nov., with the type strain CCUG 33897(T) (=CIP 107216(T)).
|
|
| 2. |
|
|
| 3. |
- Hoyles, Lesley, et al.
(författare)
-
Transfer of members of the genus Falcivibrio to the genus Mobiluncus, and emended description of the genus Mobiluncus
- 2004
-
Ingår i: SYSTEMATIC AND APPLIED MICROBIOLOGY. - : Elsevier BV. - 0723-2020. ; 27:1, s. 72-83
-
Tidskriftsartikel (refereegranskat)abstract
- It has long been thought that the genera Mobiluncus and Falcivibrio contain the same organisms. Using a polyphasic approach, it was found that Mobiluncus curtisii and Mobiluncus mulieris were the same as Falcivibrio vaginalis and Falcivibrio grandis, respectively. As the genus name Mobiluncus takes precedence, it is proposed that F. vaginalis and F. grandis be transferred to the genus Mobiluncus. In agreement with previous studies, results from phenotypic tests did not support the separation of M. curtisii strains into its two subspecies, M. curtisii subsp. curtisii and M. curtisii subsp. holmesii. Phenotypic complexity within M. curtisii dictates that the species should be treated as a complex until more in-depth analyses of the species have been performed. Phylogenetic analyses, based on 16S rRNA gene sequences, demonstrated that the genus Mobiluncus was associated with Varibaculum cambriense and the two subspecies of Actinomyces neuii, and that A. neuii is only distantly related to Actinomyces sensu stricto. Key words: Mobiluncus; polyphasic taxonomy; PFGE; 16S rDNA; Actinomycetaceae; Falcivibrio
|
|
| 4. |
- Solazzo, Caroline, et al.
(författare)
-
Modeling deamidation in sheep α-keratin peptides and application to archaeological wool textiles
- 2014
-
Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 86:1, s. 567-575
-
Tidskriftsartikel (refereegranskat)abstract
- Deamidation of glutamine (Q) and asparagine (N) has been recognized as a marker of degradation and aging in ancient proteins. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to study deamidation in wool textiles, we identified eight peptides from α-keratin proteins in sheep wool that could potentially be used to assess the level of degradation. For each chosen peptide, the extent of deamidation was determined by comparing the calculated theoretical distribution with the measured distribution using a genetic algorithm that gives the best fit to the measured distribution. Variations in the levels of deamidation were observed between peptides and in modern wool samples buried for up to 8 years in which deamidation levels were relatively low under short-term burial. In contrast, deamidation was higher in archeological textile fragments from medieval sites ranging from the 9th to 13th century in York (United Kingdom) and Newcastle (United Kingdom) and from the 13th to 16th century in Reykholt (Iceland). Major differences were observed between the British and the Icelandic samples, showing a negative correlation between age of samples and levels of deamidation, but highlighting the effect of local environment. In addition, nanoscale liquid chromatography–electrospray ionization tandem mass spectrometry (nanoLC–ESI-MS/MS) data indicated that deamidation in wool’s α-keratin was influenced by primary and higher-order structures. Predominance of deamidation on glutamine rather than asparagine in the archeological samples was attributed to a higher abundance of Q in the α-helical core domain of keratins, neighboring residues and steric hindrance preventing deamidation of N.
|
|
| 5. |
- Solazzo, Caroline, et al.
(författare)
-
Proteomic evaluation of the biodegradation of wool fabrics in experimental burials
- 2013
-
Ingår i: International Biodeterioration & Biodegradation. - : Elsevier BV. - 0964-8305. ; 80, s. 48-59
-
Tidskriftsartikel (refereegranskat)abstract
- Woollen textiles recovered from archaeological excavations are usually fragile, discoloured, mineralised, or highly biodeteriorated fragmentary remains. The nature and extent of preservation is highly dependent on the site of burial and factors such as soil composition, pH, temperature, oxygen content, and contact with a wood coffin or metals. Understanding the particular biodegradation in archaeological sites is important for biomolecular studies of textiles, and to assist in the conservation of these finds. Wool fabrics dyed and buried for up to 8 yr in bog-type soils in Denmark (Lejre) and Norway (Rørmyra), and in marine sediments in Sweden (Marstrand) were evaluated by proteomics analysis. Wool degradation was found to occur through a range of differing mechanisms, mainly due to the complex nature of wool itself with its many families of proteins (keratin and keratin-associated proteins) and structures. Microbial activity was a large contributory factor to the physical deterioration of the wool fabrics at Lejre and Marstrand, and might result in faster loss of keratin-associated proteins over keratins. Additional hydrolysis took place at Marstrand, influenced by the environmental conditions of the sediment, and in particular the alkaline pH, contributing to the degradation of keratins. However, cross-linking was associated with the long-term preservation of the fabrics at Rørmyra, where pH, temperature, and vegetative composition of the bog prevented microbial activity, and sphagnum moss might preserve wool by binding with keratins.
|
|
| 6. |
- Blösch, Günter, et al.
(författare)
-
Twenty-three unsolved problems in hydrology (UPH) - a community perspective
- 2019
-
Ingår i: Hydrological Sciences Journal. - : Informa UK Limited. - 0262-6667 .- 2150-3435. ; 64:10, s. 1141-1158
-
Tidskriftsartikel (refereegranskat)abstract
- This paper is the outcome of a community initiative to identify major unsolved scientific problems in hydrology motivated by a need for stronger harmonisation of research efforts. The procedure involved a public consultation through online media, followed by two workshops through which a large number of potential science questions were collated, prioritised, and synthesised. In spite of the diversity of the participants (230 scientists in total), the process revealed much about community priorities and the state of our science: a preference for continuity in research questions rather than radical departures or redirections from past and current work. Questions remain focused on the process-based understanding of hydrological variability and causality at all space and time scales. Increased attention to environmental change drives a new emphasis on understanding how change propagates across interfaces within the hydrological system and across disciplinary boundaries. In particular, the expansion of the human footprint raises a new set of questions related to human interactions with nature and water cycle feedbacks in the context of complex water management problems. We hope that this reflection and synthesis of the 23 unsolved problems in hydrology will help guide research efforts for some years to come.
|
|
