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Träfflista för sökning "WFRF:(Freitag Camilla) ;hsvcat:3"

Search: WFRF:(Freitag Camilla) > Medical and Health Sciences

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1.
  • McGinn, Steven, et al. (author)
  • New Technologies for DNA analysis-A review of the READNA Project.
  • 2016
  • In: New Biotechnology. - : Elsevier BV. - 1876-4347 .- 1871-6784.
  • Research review (peer-reviewed)abstract
    • The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 4 1/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3(rd) and 4(th) generation of sequencing methods with nanopores and in situ sequencing, respectively.
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2.
  • Beech, Jason P., et al. (author)
  • Sample preparation for single-cell whole chromosome analysis
  • 2012
  • In: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - 9780979806452 ; , s. 998-999
  • Conference paper (peer-reviewed)abstract
    • In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DNA in one piece and transferring it to a nano-channel for visualisation. In order to achieve control over the bacteria we encapsulate them in agarose, using flow focusing. The encapsulated bacteria can then be transported in microchannels to proximity with the nanochannels and then chemically lysis can be performed. Following lysis the intact genome can be extracted and transferred to the meandering nanochannel for analysis. We believe this device holds the potential to significantly decrease analysis times for single cell, whole genome analysis with the potential of opening up for automated, high-throughput genome analysis in microfluidic systems.
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