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- Tibiletti, Tania, 1984-, et al.
(författare)
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The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803 : Transcriptomic, proteomic and metabolomic analyses reveal new insights into their function
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- The five small CAB-like proteins (ScpA-E) of the cyanobacterium Synechocystis sp. PCC 6803 consist of one transmembrane helix and belong to the light-harvesting like family. While in wild type they are induced by various stresses, in cells with Photosystem I (PSI) -less background the SCPs are constitutively expressed. Here we performed a detailed comparison of a PSI-less control strain with a strain depleted of PSI and all SCPs, with the aim to elucidate the function of the SCPs within Photosystem II. Electron microscopical immages showed a drastic alteration of the cellular organization, visible by a rough surface and few thylakoid membranes in the PSI-less/ScpABCDE- strain. Immunoblots pointed to a decreased amount of PSII per cell volume. Deletion of the scp genes also affected the carbon- and nitrogen-balance and thereby the carbohydrate accumulation at the expenses of protein- and chlorophyll-synthesis as judged by transcriptomic and metabolomic analyses. Our results show that SCPs ensure a correct assembly/repair of PSII and therefore are important for the adaptation to environmental stresses - especially during the generation of reactive oxygen species.
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