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- Conze, Lei Liu, et al.
(författare)
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Construction and deep sequencing of cDNA libraries representing medium-sized RNA
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Transcriptome analysis has recently become feasible with the application of RNA-seq, the high-throughput sequencing analysis of RNAs through cDNA synthesis. Current transcriptome analysis approaches are limited to analysis of long (>200 nts) or short (~20 nts) RNAs. Here we describe an approach that can be used for the analysis of the medium-sized (~200 nts) RNA transcriptome, which reveals the full-length sequence of RNAs at the single molecule level. Our approach can easily be adapted to generate libraries that are suitable for sequencing by several of the currently used next-generation platforms.
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