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- Cöster, Mathias, 1969-, et al.
(författare)
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Digital Transformation : Understanding Business Goals, Risks, Processes, and Decisions
- 2023
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Även om digitalisering är långt ifrån ett nytt koncept, antar många att det räcker med att bara införa automation och informationssystem i olika former för att effektivisera deras organisations verksamhet. Denna missuppfattning kan ofta leda till oordning och kostsamma misstag. Digital transformation: Att förstå affärsmål, risker, processer och beslut visar hur man undviker sådana problem genom att noggrant överväga vad ett företag verkligen behöver.Till skillnad från många andra böcker om digital transformation, uppehåller sig författarna inte vid databasdesign eller detaljerna för att implementera informationssystem. Istället betonar de vikten av en tydlig förståelse för alla aspekter av en organisation för att effektivt kunna implementera och hantera digitala system, från affärsmål och strategier till att strukturera information och fatta beslut, riskbedömningar, projektledning, organisera och upphandla tjänster och Produkter.Den här boken är organiserad i elva kapitel och bygger på exempel från hela världen och kommer att vara av intresse för universitetsstudenter inom företagsekonomi, management, informationssystem och datavetenskap, såväl som praktiker som vill bättre förstå hur man hanterar digital transformation i sin egen organisation.
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- de Oliveira, Felipe Marques Souza
(författare)
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Development and Application of Proximity Assays for Proteome Analysis in Medicine
- 2018
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications.In paper I, we present an adaptation of the solid-phase proximity ligation assay (SP-PLA) for the detection of post-translational modification of proteins (PTMs). The assay was adapted for the detection of two of the most commons PTMs present in proteins, glycosylation and phosphorylation, offering the encouraging prospect of using detection of PTMs in a diagnostic or prognostic capacity. In paper II, we developed a variant of the proximity ligation assay using micro titer plate for detection and quantification of protein using optical density as readout in the fluorometer, termed PLARCA. With a detection limit considerably lower than ELISA, PLARCA detected femtomolar levels of these proteins in patient samples.In paper III, we aim to compare detection values of samples collected from earlobe capillary, venous plasma, as well as capillary plasma stored in dried plasma spots (DPS) assessed with a 92-plex inflammation panel using multiplex proximity extension assay (PEA). Despite the high variability in protein measurements between the three sample sources, we were able to conclude that earlobe capillary sampling is a suitable less invasive alternative, to venipuncture.In paper IV, we describe the application of PLARCA and proximity extension assay (PEA) for the detection of GAD65 autoantibodies (GADA). Thus, offering highly sensitive and specific autoimmunity detection.
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- Gullberg, Mats, et al.
(författare)
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Cytokine detection by antibody-based proximity ligation
- 2004
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 101:22, s. 8420-4
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Efficient and precise detection techniques, along with extensive repertoires of specific binding reagents, will be needed to meet the challenges of proteome analyses. The recently established proximity ligation mechanism enables sensitive high-capacity protein measurements by converting the detection of specific proteins to the analysis of DNA sequences. Proximity probes containing oligonucleotide extensions are designed to bind pairwise to target proteins and to form amplifiable tag sequences by ligation when brought in proximity. In our previous report, both the ligatable arms and the protein binders were DNA molecules. We now generalize the method by providing simple and convenient protocols to convert any polyclonal antibodies or matched pair of monoclonal antibodies to proximity probe sets through the attachment of oligonucleotide sequences. Sufficient reagent for >100,000 proximity ligation assays can be prepared from 1 microg of antibody. The technique is applied to measure cytokines in a homogenous test format with femtomolar detection sensitivities in 1-microl samples, and we exemplify its utility in situations when only minute sample amounts are available.
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- Gullberg, Mats, 1953-
(författare)
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Health care professionals' self description
- 1996
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The aim of the present thesis was to survey and analyse professional self of health care professionals, focusing on perceptions of self, and on their attitudes towards aspects of sense of responsibility.Studies were accomplished with quantitative techniques using questionnaires and/or instruments. A total of 803 respondents was included, and the first study (I) presented the development of the PSDF and comprised 30 registered nurses. The next study (II) examined results from 332 medical laboratory technologists who answered the PSDF. In the third study (III) 68 occupational therapists responded to the PSDF. The fourth study (IV) was a six-year follow-up of 43 registered nurses from the beginning of their training to three years after graduation, and they responded to the PSDF, the SRF and to the Form for ranked sources of encouragement. The fifth study (V) comprised four professional groups from a university hospital in Sweden (physicians, physiotherapists, registered nurses and enrolled nurses), and 312 responded to the set of forms (the PSDF, the SRF, and the Form for ranked sources of encouragement).The main theoretical approach was based on symbolic interactionism, where the self is a significant notion. Concepts on attitude, sense of responsibility and encouragement were important.The results showed that there are components of self description that is 'specific to one professional group, but also components that uncover similarities. Three important components of professional self were outlined; professional conduct, work ability and empathy. Important issues were also encouragement for work done, which influences the professional self, and that the positive attitudes to sense of responsibility showed similarities with characteristics of professional self.In conclusion, two propositions were addressed: that professionals' self description is composed of three meaningful dimensions; professional conduct, work ability and empathy, and that the professional self can be improved throughdevelopment of intrinsic attitudes and behaviours.
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- Gullberg, Mats, 1971-
(författare)
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Proximity Ligation as a Universal Protein Detection Tool
- 2003
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Among the great challenges in biology are the precise quantification of specific sets of proteins and analyses of their patterns of interaction on a much larger scale than is possible today.This thesis presents a novel protein detection technique - proximity ligation - and reports the development and application of a nucleic acid amplification technique, RCA. Proximity ligation converts information about the presence or co-localization of specific proteins to unique sets of nucleic acid sequences. For detection of target proteins or protein complexes the coincident binding by pairs or triplets of specific protein-binding reagents are required. Oligonucleotide-extensions attached to those binding reagents are joined by a DNA ligase and subsequently analyzed by standard molecular genetic techniques. The technique is shown to sensitively detect an assortment of proteins using different types of binders converted to proximity probes, including SELEX aptamers and mono- and polyclonal antibodies. I discuss factors important for using the technique to analyze many proteins simultaneously.Quantification of target molecules requires precise amplification and detection. I show how rolling circle amplification, RCA, can be used for precise quantification of circular templates using modified molecular beacons with real-time detection. The combination of proximity-probe templated circularization and RCA results in a sensitive method with high selectivity, capable of visualizing individual immobilized proteins. This technique is used for localized detection of a set of individual proteins and protein complexes at sub-cellular resolution.
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- Hindström, Mats, et al.
(författare)
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Strategi för formellt skydd av skog i Gävleborgs län. D. 3, Skogliga värdetrakter i Gävleborgs län
- 2006
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Värdetraktsanalys - skogliga värdetrakter i Gävleborgs län.Med värdetrakt avses ett landskapsavsnitt med särskilt höga ekologiska bevarandevärden.Länsstyelsen har analyserat den geografiska fördelningen i länet av kända värdekärnor.De kända värdekärnona består av tre olika kategorier:nyckelbiotoper,naturreservat och nationalparker samt områden som Länsstyrelsen inventerat och hittat höga skogliga naturvärden.
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