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Performance in omic...
Performance in omics analyses of blood samples in long-term storage : opportunities for the exploitation of existing biobanks in environmental health research
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Hebels, Dennie G. A. J. (author)
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Georgiadis, Panagiotis (author)
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Keun, Hector C. (author)
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Athersuch, Toby J. (author)
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Vineis, Paolo (author)
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Vermeulen, Roel (author)
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Portengen, Lützen (author)
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- Bergdahl, Ingvar A. (author)
- Umeå universitet,Enheten för biobanksforskning,Yrkes- och miljömedicin,Arcum
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- Hallmans, Göran (author)
- Umeå universitet,Enheten för biobanksforskning,Näringsforskning
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Palli, Domenico (author)
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Bendinelli, Benedetta (author)
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Krogh, Vittorio (author)
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Tumino, Rosario (author)
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Sacerdote, Carlotta (author)
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Panico, Salvatore (author)
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Kleinjans, Jos C. S. (author)
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de Kok, Theo M. C. M. (author)
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Smith, Martyn T. (author)
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Kyrtopoulos, Soterios A. (author)
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(creator_code:org_t)
- Environmental Health Perspectives, 2013
- 2013
- English.
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In: Journal of Environmental Health Perspectives. - : Environmental Health Perspectives. - 0091-6765 .- 1552-9924. ; 121:4, s. 480-487
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
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- Background: The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown.Objectives: We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles.Methods: We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for ≤ 24 hr before fractionating them into buffy coat, erythrocytes, and plasma and freezing the fractions at -80oC or in liquid nitrogen. We developed methodology for isolating RNA from the buffy coats and conducted omic analyses. Finally, we analyzed analogous samples from the EPIC-Italy and Northern Sweden Health and Disease Study biobanks.Results: Microarray-quality RNA could be isolated from buffy coats (including most biobank samples) that had been frozen within 8 hr of blood collection by thawing the samples in RNA preservative. Different anticoagulants influenced the metabolomic, proteomic, and to a lesser extent transcriptomic profiles. Transcriptomic profiles were most affected by the delay (as little as 2 hr) before blood fractionation, whereas storage temperature had minimal impact. Effects on metabolomic and proteomic profiles were noted in samples processed ≥ 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years.Conclusions: Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Arbetsmedicin och miljömedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Occupational Health and Environmental Health (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Public Health, Global Health, Social Medicine and Epidemiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- biomarkers
- epigenomics
- metabolomics
- metabonomics
- molecular epidemiology
- proteomics
- transcriptomics
Publication and Content Type
- ref (subject category)
- art (subject category)
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Hebels, Dennie G ...
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Georgiadis, Pana ...
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Keun, Hector C.
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Athersuch, Toby ...
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Vineis, Paolo
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Vermeulen, Roel
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show more...
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Portengen, Lütze ...
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Bergdahl, Ingvar ...
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Hallmans, Göran
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Palli, Domenico
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Bendinelli, Bene ...
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Krogh, Vittorio
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Tumino, Rosario
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Sacerdote, Carlo ...
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Panico, Salvator ...
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Kleinjans, Jos C ...
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de Kok, Theo M. ...
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Smith, Martyn T.
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Kyrtopoulos, Sot ...
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Umeå University