| 1. |
- Ingvarsson, Pär K., et al.
(author)
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Towards integration of population and comparative genomics in forest trees
- 2016
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In: New Phytologist. - : Wiley. - 0028-646X .- 1469-8137. ; 212:2, s. 338-344
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Research review (peer-reviewed)abstract
- The past decade saw the initiation of an ongoing revolution in sequencing technologies that is transforming all fields of biology. This has been driven by the advent and widespread availability of high-throughput, massively parallel short-read sequencing (MPS) platforms. These technologies have enabled previously unimaginable studies, including draft assemblies of the massive genomes of coniferous species and population-scale resequencing. Transcriptomics studies have likewise been transformed, with RNA-sequencing enabling studies in nonmodel organisms, the discovery of previously unannotated genes (novel transcripts), entirely new classes of RNAs and previously unknown regulatory mechanisms. Here we touch upon current developments in the areas of genome assembly, comparative regulomics and population genetics as they relate to studies of forest tree species.
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| 2. |
- Mackay, John, et al.
(author)
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Towards decoding the conifer giga-genome
- 2012
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In: Plant Molecular Biology. - : Springer. - 0167-4412 .- 1573-5028. ; 80:6, s. 555-569
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Research review (peer-reviewed)abstract
- Several new initiatives have been launched recently to sequence conifer genomes including pines, spruces and Douglas-fir. Owing to the very large genome sizes ranging from 18 to 35 gigabases, sequencing even a single conifer genome had been considered unattainable until the recent throughput increases and cost reductions afforded by next generation sequencers. The purpose of this review is to describe the context for these new initiatives. A knowledge foundation has been acquired in several conifers of commercial and ecological interest through large-scale cDNA analyses, construction of genetic maps and gene mapping studies aiming to link phenotype and genotype. Exploratory sequencing in pines and spruces have pointed out some of the unique properties of these giga-genomes and suggested strategies that may be needed to extract value from their sequencing. The hope is that recent and pending developments in sequencing technology will contribute to rapidly filling the knowledge vacuum surrounding their structure, contents and evolution. Researchers are also making plans to use comparative analyses that will help to turn the data into a valuable resource for enhancing and protecting the world's conifer forests.
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| 3. |
- Rosvall, Ola, et al.
(author)
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Using Norway spruce clones in Swedish forestry : implications of clones for management
- 2019
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In: Scandinavian Journal of Forest Research. - : Taylor & Francis. - 0282-7581 .- 1651-1891. ; 34:5, s. 390-404
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Research review (peer-reviewed)abstract
- This final paper of the series discusses implications of various aspects of using clones in forestry. Benefits from using clones are considerable. A large benefit arises from the ability to deploy genetic gain much sooner than is possible through conventional seed orchards. This benefit applies even to the use of clones to implement family forestry, even though the clones themselves are not tested. The requirement for genetic diversity at both the stand and landscape levels requires active management to ensure that diversity is conserved. This is achieved partly through the management of breeding populations, as well as by managing the genetic diversity and number of genotypes deployed in clone mixtures. A numerical example is given comparing diversity of clone and seed orchard deployment over time. Many aspects of managing concerns about using clones are about communication to clarify public perceptions and establishing a code of practice.
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