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Structural study of...
Structural study of the X-ray-induced enzymatic reduction of molecular oxygen to water by Steccherinum murashkinskyi laccase : insights into the reaction mechanism
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- Polyakov, K. M. (författare)
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str. 32, Moscow 119991, Russian Federation
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- Gavryushov, S. (författare)
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str. 32, Moscow 119991, Russian Federation
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- Ivanova, Svetlana (författare)
- Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV)
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- Fedorova, T. V. (författare)
- A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Ave. 33/2, Moscow 119071, Russian Federation
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- Glazunova, O. A. (författare)
- A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Ave. 33/2, Moscow 119071, Russian Federation
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- Popov, A. N. (författare)
- ESRF - The European Synchrotron, CS 40220, 38043 Grenoble CEDEX 9, France
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- Koroleva, O. V. (författare)
- A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Ave. 33/2, Moscow 119071, Russian Federation
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Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str 32, Moscow 119991, Russian Federation Institutionen för biomedicinsk vetenskap (BMV) (creator_code:org_t)
- International Union of Crystallography, 2017
- 2017
- Engelska.
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Ingår i: Acta Crystallographica Section D. - : International Union of Crystallography. - 2059-7983. ; 73:Pt 5, s. 388-401
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The laccase from Steccherinum murashkinskyi is a member of the large family of multicopper oxidases that catalyze the oxidation of a wide range of organic and inorganic substrates, accompanied by the reduction of dioxygen to water. The reducing properties of X-ray radiation and the high quality of the laccase crystals allow the study of the catalytic reduction of dioxygen to water directly in a crystal. A series of diffraction data sets with increasing absorbed radiation dose were collected from a single crystal of Steccherinum murashkinskyi laccase at 1.35 angstrom resolution. Changes in the active-site structure associated with the reduction of molecular oxygen to water on increasing the absorbed dose of ionizing radiation were detected. The structures in the series are mixtures of different states of the enzyme-substrate complex. Nevertheless, it was possible to interpret these structures as complexes of various oxygen ligands with copper ions in different oxidation states. The results allowed the mechanism of oxygen reduction catalyzed by laccases to be refined.
Nyckelord
- laccase
- serial X-ray data
- enzymatic oxygen reduction
- photo-electron reduction
- reaction mechanisms
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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