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- Chen, R., et al.
(författare)
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A low cost surface plasmon resonance biosensor using a laser line generator
- 2015
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Ingår i: Optics Communications. - : Elsevier BV. - 0030-4018 .- 1873-0310. ; 349, s. 83-88
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Tidskriftsartikel (refereegranskat)abstract
- Due to the instrument designed by using a common surface plasmon resonance biosensor is extremely expensive, we established a portable and cost-effective surface plasmon resonance biosensing system. It is mainly composed of laser line generator, P-polarizer, customized prism, microfluidic cell, and line Charge Coupled Device (CCD) array. Microprocessor PIC24FJ128GA006 with embedded A/D converter, communication interface circuit and photoelectric signal amplifier circuit are used to obtain the weak signals from the biosensing system. Moreover, the line CCD module is checked and optimized on the number of pixels, pixels dimension, output amplifier and the timing diagram. The micro-flow cell is made of stainless steel with a high thermal conductivity, and the microprocessor based Proportional-Integral-Derivative (PID) temperature-controlled algorithm was designed to keep the constant temperature (25 °C) of the sample solutions. Correspondingly, the data algorithms designed especially to this biosensing system including amplitude-limiting filtering algorithm, data normalization and curve plotting were programmed efficiently. To validate the performance of the biosensor, ethanol solution samples at the concentrations of 5%, 7.5%, 10%, 12.5% and 15% in volumetric fractions were used, respectively. The fitting equation ΔRU=-752987.265+570237.348×RI with the R-Square of 0.97344 was established by delta response units (ΔRUs) to refractive indexes (RI). The maximum relative standard deviation (RSD) of 4.8% was obtained.
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| 2. |
- Hu, J., et al.
(författare)
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Detection of clenbuterol hydrochloride residuals in pork liver using a customized surface plasmon resonance bioanalyzer
- 2015
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 10:3
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Tidskriftsartikel (refereegranskat)abstract
- A surface plasmon resonance (SPR) immunoassay with an immobilization of self-assembled molecular identification membrane for the detection of residual Clenbuterol Hydrochloride (CLB) in pork liver was systematically investigated and experimentally validated for its high performance. SPR immunoassay with a regular competitive inhibition assay cannot be directly verified to detect CLB residuals. In this study, the binding of Au film with mercaptopropionic acid was investigated using the known form of the strong S-Au covalent bonds formed by the chemical radical of the mercaptopropionic acid and the Au film. After that, the immunoglobulin IgG of swine (SwIgG-CLB) was bonded with the mercaptopropionic acid by covalent -CO-NH- amide bonding. The modified comprehensive analysis of how the membrane structure works was introduced together with the customized SPR bioanalyzer. In order to evaluate the performance of this biomembrane structure, the concentrations of CLBcontained solutions of 0 ng•mL-1, 10 ng•mL-1, 20 ng•mL-1, 33.3 ng•mL-1, and 40 ng•mL-1 were prepared by adding CLB reagents into the solutions of CLB antibody (Clenbuterol Hydrochloride Antibody, CLB-Ab), successively and then the response unit (RU) was measured individually. Using the data collected from the linear CCD array, the fitting curve was established with the R-Square value of 0.9929. Correspondingly, the recovery rate ranged from 88.48% to 103.21% was experimented and the limit of detection of CLB in 1.26 ng•mL-1 was obtained efficiently. It was concluded that the detection method associated with biomembrane properties is expected to contribute much to the determination of residual CLB in pork liver quantitatively by using the customized SPR bioanalyzer. © 2015 Hu et al.
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| 3. |
- Jiang, Bin, et al.
(författare)
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Geospatial Analysis and Modeling of Urban Structure and Dynamics: An Overview
- 2010
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Ingår i: Geospatial Analysis and Modelling of Urban Structure and Dynamics. - Dordrecht : Springer Nature. ; , s. 3-11, s. 3-11
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Bokkapitel (refereegranskat)abstract
- Geographic information research and technologies have experienced over four decades of development, from the mainframe to the workstation to the desktop, and to today’s laptop and mobile devices. Every important GIS development is driven by a significant breakthrough of mainstream information technology. For example, the 1980s was characterized by the popularity of personal computers that were increasingly becoming affordable to university departments, governmental agencies and private sectors. Many university GIS programs were established during this time period, and the NSF-funded NCGIA played an important role in coordinating the development of course curriculum and related research activities. The next decade can be named the age of GIScience. GIScience is the science behind GISystens, dealing with fundamental questions raised by the use of GISystems and technologies (Goodchild 1997). It occurred at the time when the Internet and the World Wide Web started to change the way we led our lives and ran our businesses. It was the Internet and the Web that made the GIS community think of a service oriented approach to GIS, namely GIServices (Günther and Müller 1999). Instead of owning a GIS, end users can be served by GIS functionalities from a remote GIService center. GIServices aim to develop distributed or decentralized GIS to serve individuals and communities for spatial planning and decision making, as well as for their daily life. Another perspective of GIS is GIStudies for studying the impacts of geographic information and technologies on society.
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