| 1. |
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| 2. |
- Eklund, D. Magnus, et al.
(författare)
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Homologues of the Arabidopsis thaliana SHI/STY/LRP1 genes control auxin biosynthesis and affect growth and development in the moss Physcomitrella patens
- 2010
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Ingår i: Development. - : The Company of Biologists. - 0950-1991 .- 1477-9129. ; 137:8, s. 1275-1284
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Tidskriftsartikel (refereegranskat)abstract
- The plant hormone auxin plays fundamental roles in vascular plants. Although exogenous auxin also stimulates developmental transitions and growth in non-vascular plants, the effects of manipulating endogenous auxin levels have thus far not been reported. Here, we have altered the levels and sites of auxin production and accumulation in the moss Physcomitrella patens by changing the expression level of homologues of the Arabidopsis SHI/STY family proteins, which are positive regulators of auxin biosynthesis genes. Constitutive expression of PpSHI1 resulted in elevated auxin levels, increased and ectopic expression of the auxin response reporter GmGH3pro:GUS, and in an increased caulonema/chloronema ratio, an effect also induced by exogenous auxin application. In addition, we observed premature ageing and necrosis in cells ectopically expressing PpSHI1. Knockout of either of the two PpSHI genes resulted in reduced auxin levels and auxin biosynthesis rates in leafy shoots, reduced internode elongation, delayed ageing, a decreased caulonema/chloronema ratio and an increased number of axillary hairs, which constitute potential auxin biosynthesis sites. Some of the identified auxin functions appear to be analogous in vascular and non-vascular plants. Furthermore, the spatiotemporal expression of the PpSHI genes and GmGH3pro:GUS strongly overlap, suggesting that local auxin biosynthesis is important for the regulation of auxin peak formation in non-vascular plants.
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| 3. |
- Eliasson, Mattias, et al.
(författare)
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Strategy for optimizing LC-MS data processing in Metabolomics : A design of experiments approach
- 2012
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 84:15, s. 6869-6876
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Tidskriftsartikel (refereegranskat)abstract
- A strategy for optimizing LC-MS metabolomics data processing is proposed. We applied this strategy on the XCMS open source package written in R on both human and plant biology data. The strategy is a sequential design of experiments (DoE) based on a dilution series from a pooled sample and a measure of correlation between diluted concentrations and integrated peak areas. The reliability index metric, used to define peak quality, simultaneously favors reliable peaks and disfavors unreliable peaks using a weighted ratio between peaks with high and low response linearity. DoE optimization resulted in the case studies in more than 57% improvement in the reliability index compared to the use of the default settings. The proposed strategy can be applied to any other data processing software involving parameters to be tuned, e.g., MZmine 2. It can also be fully automated and used as a module in a complete metabolomics data processing pipeline.
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| 4. |
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| 5. |
- Hansson, Magnus L., et al.
(författare)
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Artificial spider silk supports and guides neurite extension in vitro
- 2021
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Ingår i: The FASEB Journal. - : John Wiley & Sons. - 0892-6638 .- 1530-6860. ; 35:11
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Tidskriftsartikel (refereegranskat)abstract
- Surgical intervention with the use of autografts is considered the gold standard to treat peripheral nerve injuries. However, a biomaterial that supports and guides nerve growth would be an attractive alternative to overcome problems with limited availability, morbidity at the site of harvest, and nerve mismatches related to autografts. Native spider silk is a promising material for construction of nerve guidance conduit (NGC), as it enables regeneration of cm-long nerve injuries in sheep, but regulatory requirements for medical devices demand synthetic materials. Here, we use a recombinant spider silk protein (NT2RepCT) and a functionalized variant carrying a peptide derived from vitronectin (VN-NT2RepCT) as substrates for nerve growth support and neurite extension, using a dorsal root ganglion cell line, ND7/23. Two-dimensional coatings were benchmarked against poly-d-lysine and recombinant laminins. Both spider silk coatings performed as the control substrates with regards to proliferation, survival, and neurite growth. Furthermore, NT2RepCT and VN-NT2RepCT spun into continuous fibers in a biomimetic spinning set-up support cell survival, neurite growth, and guidance to an even larger extent than native spider silk. Thus, artificial spider silk is a promising biomaterial for development of NGCs.
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| 6. |
- Olsson, Jörgen, et al.
(författare)
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Landscape and substrate properties affect species richness and community composition of saproxylic beetles
- 2012
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Ingår i: Forest Ecology and Management. - : Elsevier. - 0378-1127 .- 1872-7042. ; 286, s. 108-120
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Tidskriftsartikel (refereegranskat)abstract
- Intensive forest management has dramatically reduced the area of old-growth forest in Fennoscandia. We examined if the proportion of old forest in a landscape affects species composition, richness and abundance of saproxylic beetles. We used tube-shaped window traps in five pairs of sites, selected so that the sites within each pair differed with respect to the proportion of old forest (>125 years) in the surrounding landscape. A landscape level inventory of the wood fungi Fomitopsis rosea, as a proxy for forest with high conservation values, was used to complement the data on old forests. In addition, to testing whether mycelia-colonised wood may attract saproxylic beetles, the tube-shaped window traps were baited with wood colonised by Fomitopsis pinicola or F. rosea. Old-forest-rich landscapes supported significantly more species and a higher abundance of saproxylic beetles than old-forest-poor landscapes. The analysis revealed a clear connection between the community composition of saproxylic beetles and the proportion of old forest and number of F. rosea fruiting bodies in the surrounding landscape (radius 3 km). The local landscape species pool thus appears to be important for the beetle species that are trapped since the composition of saproxylic beetles differed between the two landscape types. The effects of the different baits were less pronounced than the effect of landscape type, although species-specific responses to the two mycelia baits were observed. This indicates that volatiles from mycelia of wood-decaying fungi and the mycelial community may affect colonisation patterns of saproxylic beetles. Our results suggest that forest fragmentation and habitat loss have resulted in depauperate beetle faunas in old-forest-poor landscapes. Our results highlight the need to invoke a landscape scale approach for preserving biodiversity, in this case the need to maintain a sufficient proportion of forest with old growth characteristics in the managed landscape.
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| 7. |
- Persson Osowski, Christine, 1978-, et al.
(författare)
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From Old Habits to New Routines : A Case Study of Food Waste Generation and Reduction in Four Swedish Schools
- 2022
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Ingår i: Resources. - : MDPI. - 2079-9276. ; 11:1
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Tidskriftsartikel (refereegranskat)abstract
- Public food service organizations are large producers of food waste, which leads to greenhouse gas emissions and the waste of natural resources. The aim of the present article was to gain insight into reasons for food waste and possible solutions for lowering food waste in schools in Sweden. In order to do so, food waste quantification in school canteens in two Swedish municipalities and nine qualitative interviews with key actors were conducted. Both municipalities displayed a high degree of variation in food waste, but the common pattern was that serving waste constituted the largest fraction of food waste, followed by plate waste and storage waste, as well as a gradual decrease in food waste over time. Food waste was mainly a result of old, disadvantageous habits, such as overproduction due to forecasting difficulties, whereas new, better routines such as serving fewer options, better planning, and a less stressful environment are the key to lowering food waste. Because food waste varies from one case to the next, it becomes important to identify and measure the causes of food waste in each school in order to be able to establish tailor-made, conscious, and flexible food waste mitigation routines.
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| 8. |
- Rönnberg, Jonas, et al.
(författare)
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Incidence of Heterobasidion spp. following different thinning regimes in Norway spruce in southern Sweden
- 2013
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Ingår i: Forest Ecology and Management. - : Elsevier BV. - 0378-1127 .- 1872-7042. ; 289, s. 409-415
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Tidskriftsartikel (refereegranskat)abstract
- The incidence of Heterobasidion spp. infections following different thinning regimes in Norway spruce was evaluated on 12 plots in a trial in southern Sweden. The plots were established in 1961 and followed until final felling in 2001. The treatments ranged from one light thinning at the experimental set-up to six thinnings. The average incidence of decay was 44% across all thinning regimes of which Heterobasidion spp. was identified in 85% of the cases. There was no difference in the incidence of infections, decayed volumes, and the size or number of Heterobasidion spp. genets among the thinning regimes. The largest genet was though found on the plot with just one light thinning and encompassed 25 trees. On average, 11.1% of the stem volume was decayed and the average height of the decay column was 4 m. Decay incidence was 3.7 times the decayed volume and the height of the decay columns was 19 times the diameter of the decay at stump height. Conclusively, early thinning appears to be more important for disease development than subsequent multiple thinning. To restrict the disease development in the stand later during the rotation period, it is of utmost importance that the earlier thinnings are made during winter conditions or otherwise properly protected against spore infection of Heterobasidion. (C) 2012 Elsevier B.V. All rights reserved.
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