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- Backius, Stefan
(författare)
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Arbetare på scen : amatörteater som politiskt verktyg
- 2011
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis focuses on the political dimensions of aesthetic expression during the Long Sixties. The thesis deals with amateur theatre ventures both within and linked to the social democratic labour movement. ’Spelet om Norbergsstrejken’ (The Play about the Norberg Strike) had its première in 1977 in a small industrial village in the industrial region of Bergslagen. Similar plays appeared in many regions of the country and a wave of workers’ plays emerged and made an impact on the internal investments of the educational association ABF in amateur theatre. The empiricism of the thesis concludes in 1982 when a social democratic amateur theatre association was founded and after a breakaway from the social democratic movement was establishing a residential study centre in another small village in Bergslagen. Sixties radicalisation provides the social context of the study and the perspective of sociological social movement research is used and developed. Based on the perspective of cognitive practice and the concepts of cosmology and movement ideology attention is directed towards the theatre assets of performance hosts, expectation horizons and patterns of behaviour. The thesis argues for a deeper understanding of sixties radicalisation partly meaning that the periodisation needs to be extended backwards as well as forwards in terms of time and partly that the political dimensions of aesthetic expression should be focused on. Based upon the results of the thesis the concept of culturactivism was formulated which defines the specific approach that appeared in the space between political and cultural activism. This highlights the need for a concept that covers the cognitive free space that arose between aesthetic expression and political activism and which has not previously attracted the attention of historical studies about sixties radicalisation to any great degree.
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| 2. |
- Johansson, Roger
(författare)
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Tracking the Mind's Eye : Eye movements during mental imagery and memory retrieval
- 2013
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis investigates the relationship between eye movements, mental imagery and memory retrieval in four studies based on eye-tracking experiments. The first study is an investigation of eye movements during mental imagery elicited both visually and verbally. The use of complex stimuli and the development of a novel method where eye movements are recorded concurrently with verbal data enabled the above-mentioned relationship to be studied to an extent going beyond what previous research had been able to do. Eye movements were found to closely reflect content and spatial layout while participants were listening to a spoken scene description, while they were describing the same scene from memory, and while they were describing a picture they had previously seen. This effect was equally strong during recall from memory irrespective of whether the scene visualised had originally been inspected visually by the participants or whether it was constructed whole-cloth from long-term memory (on the basis of a spoken scene description that the participants had previously listened to). It was also found that eye movements "to nothing" appeared both when the participants were visualising scenes while looking at a blank screen and when they were doing so in complete darkness. The second study explored an effect frequently observed in the first study, involving a "scaling-down" during recall of participants' gaze patterns to an area smaller than that occupied by the stimulus encoded. It was found that this scaling effect correlated with spatial-imagery ability: the gaze patterns of participants with weaker spatial-imagery ability were closer in size to the encoded scene than the gaze patterns of those stronger in spatial-imagery ability. In the third study, the role of eye movements during mental imagery was investigated in four experiments where eye movements were prohibited during either the encoding phase or the recall phase. Experiments 1 and 2 showed that maintaining central fixation during visual or auditory encoding, respectively, had no effect on how eye movements were executed during recall. Thus, oculomotor events during recall are not reproductions of those produced during encoding. In Experiments 3 and 4, central fixation was instead maintained during recall. This turned out to alter and impair scene recollection, irrespective of the modality of encoding. Finally, in the fourth study, the functional role of eye movements in relation to memory retrieval was further investigated by means of direct eye-movement manipulation in the retrieval phase of an episodic-memory task. Four conditions were used: (1) free viewing on a blank screen, (2) maintaining central fixation, (3) viewing within a square congruent with the location of the objects to be recalled, and (4) viewing within a square incongruent with the location of the objects to be recalled. The results obtained show that gaze position plays an active and facilitatory role during memory retrieval. The findings from these studies are discussed in the light of current theories regarding eye movements during mental imagery and memory retrieval.
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| 3. |
- Karlsson, Terese, 1979-
(författare)
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The expression and molecular functions of LRIG proteins in cancer and psoriasis
- 2013
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The leucine-rich repeats and immunoglobulin-like domains (LRIG) family consists of three integral membrane proteins that are important in human cancer. LRIG1 is a negative regulator of growth factor signaling. Its expression is associated with longer survival in several cancer types, and the gene has been shown to function as a tumor suppressor. The roles of LRIG2 and LRIG3 are less well known. The aim of this thesis was to improve our understanding of the expression and function of the LRIG protein family in psoriasis and cancer.To investigate their expression in psoriasis, the mRNA levels and subcellular localization of the LRIG proteins were analyzed and compared between normal and psoriatic human skin. There were no differences in the LRIG mRNA levels between psoriatic and normal skin samples. However, the subcellular localization of all three LRIG proteins differed between psoriatic and normal skin.To study the physiological and molecular functions of Lrig2, we generated Lrig2E12-/- mice. These mice were viable and born at a Mendelian rate, but Lrig2E12-/- mice had an increased rate of spontaneous mortality and a transient reduction in growth rate compared to Lrig2 wild-type (wt) mice. In an orthotopic platelet-derived growth factor (PDGF)B-driven brain tumor mouse model, we studied the effect of Lrig2 on gliomagenesis. All Lrig2 wt mice developed tumors; 82% developed grade II/III tumors, and 18% developed grade IV tumors. Only 77% of the Lrig2E12-/- mice developed tumors, and they were all grade II/III tumors. Thus, Lrig2 increased the incidence and malignancy rates of PDGFB-driven gliomas. We then analyzed the effect of Lrig2 on Pdgf receptor (Pdgfr) signaling. Lrig2 had no effect on Pdgfr steady-state levels, the starvation-induced up-regulation of Pdgfrs, the phosphorylation of Pdgfrs, primary cilium formation or the PDGFBB-induced phosphorylation of Akt or Erk1/2. However, the kinetics of induction of the immediate-early genes Fos and Egr2 were altered, resulting in a more rapid induction in Lrig2E12-/- cells.We then analyzed the clinical and biological importance of LRIG1 in lung cancer. In a human lung cancer tissue micro-array (TMA), LRIG1 expression was found to be an independent positive prognostic factor for adenocarcinoma. To study the importance of Lrig1 regarding lung cancer development in vivo, we used an inducible EGFRL858R-driven mouse lung cancer model. The mice developed diffuse lung adenocarcinoma, and the tumor burden was greater in Lrig1-/- mice than in Lrig1+/+ mice (p = 0.025) at 60 days. The human lung cancer cell line H1975, with either normal or Tet-induced expression of LRIG1, was injected into the flanks of Balb/cA nude mice. Tumors formed by LRIG1-overexpressing cells were smaller than those formed by parental cells, further indicating that LRIG1 is important during lung tumor formation or growth. In vitro, LRIG1 suppressed the proliferation of H1975 cells and down-regulated the phosphorylation of MET and RET.To investigate the molecular functions of LRIG proteins further, we performed a yeast two-hybrid (YTH) screen using a peptide from the cytosolic tail of LRIG3 as bait. This screen identified LMO7 and LIMCH1 as prominent interaction partners for LRIG3. Proximity ligation assays showed that LMO7 interacted with all of the LRIG proteins at endogenous expression levels. LMO7 and LIMCH1 were expressed in all human tissues analyzed. Their expression was dramatically decreased in lung cancer compared to normal lung tissue. The expression of LMO7 was analyzed in a human lung cancer TMA. LMO7 was expressed in respiratory epithelial cells in normal lungs. However, LMO7 was only expressed in a quarter of the lung tumors. LMO7 expression was found to be an independent negative prognostic factor for lung cancer.In summary, we found that the LRIG proteins were redistributed in psoriatic skin. In a mouse glioma model, Lrig2 promoted oligodendroglioma genesis. LRIG1 was an independent positive prognostic factor in human lung cancer. Lrig1 ablation increased the tumor size in an EGFRL858R-driven lung cancer mouse model. LRIG1 expression decreased the tumor growth of human lung cancer cells in a xenograft mouse model. LMO7 interacted with all three LRIG proteins and was an independent negative prognostic factor in human lung cancer. These data demonstrate the importance of LRIG proteins in human disease.
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| 4. |
- Sohail, Hafiz Muhammad
(författare)
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Experimental and Theoretical Studies of Metal Adsorbates Interacting with Elemental Semiconductor Surfaces
- 2014
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Metal adsorbates on semiconductor surfaces have been widely studied over the last few decades. The main interest is focused on various one or two-dimensional structures that exhibit interesting electronic and atomic properties. This thesis focuses on metal adsorbates interacting with the Si(111) and Ge(111) surfaces. The main experimental techniques used in the thesis include angle resolved photoelectron spectroscopy (ARPES), core-level spectroscopy, scanning tunneling microscopy (STM), and low energy electron diffraction (LEED). The experimental studies have, in some cases, been complemented by theoretical electronic structure investigations based on density functional theory (DFT).Silver (Ag), a noble metal, gives rise to several reconstructions on the (111) surfaces of Si and Ge. The Ag/Si(111) surface has been extensively studied, but the Ag/Ge(111) surface has not been given similar attention, and there are no detailed experimental nor calculated electronic band structures available in the literature. Thus, a detailed ARPES investigation of the electronic structure of the Ag/Ge(111) surface, with nominally 1 monolayer (ML) of Ag, is presented in the thesis together with its atomic structure.The Ag/Si(111) and Ag/Ge(111) surfaces were also studied by first principles DFT based calculations (WIEN2k). Two atomic models have been suggested for the surfaces in the literature, i.e., the honeycomb-chained-trimer (HCT) and the in-equivalent trimer (IET) models. Band structure calculations were performed for both models, and comparisons between calculated and experimental surface band structures are presented for the Si and Ge cases.Adding approximately 0.2 ML of Ag to Ag/Ge(111) results in a 6×6 phase. The electronic structure of the surface is presented in detail. Several new bands appear in the energy region close the Fermi level, which can all be explained by umklapp scattering by reciprocal lattice vectors of the 6×6 lattice. A metal to semiconductor transition, associated with the to 6×6 structural change, is explained by gaps opening up where the umklapp scattered bands cross.After having established sufficient understanding of the Ag/Si(111) and Ag/Ge(111) surfaces, they were used as substrates for the formation of binary surface alloys. An amount of 0.45 ML of Sn, in combination with the Ag of the Ag/Ge(111) surface, forms a well-defined xbinary alloy. The surface band structure shows some modifications compared to that of Ag/Ge(111) surface. The STM results show clearly the x periodicity.A Sn coverage of 0.75 ML on the Ag/Ge(111) surface results in a very wellordered 3×3 surface alloy. This alloy shows a very rich surface band structure in which the upper band exhibits peculiar splits. Two-dimensional constant energy contour data reveal the existence of two rotated contours which is related to the presence of split bands in certain directions. STM images show a hexagonal or a honeycomb structure depending on sample to tip bias.A similar amount of Sn (0.75 ML) was also evaporated onto the Ag/Si111) surface, with the purpose to form a surface alloy on Si(111). This resulted in a very well-ordered Sn/Ag/Si(111)2×2 periodicity. The surface shows an interesting free electron like band which crosses the Fermi level. STM images reveal clear, but differently looking, protrusions in the 2×2 unit cell when comparing empty and filled state images. The atomic structure of the surface alloy was modelled by DFT calculations using structural information provided by the STM images. The modelling resulted in a structure consisting of Sn and Ag trimers and a fourth Ag atom located at the corner of the 2×2 cell. In addition, the calculated electronic structure based on the proposed model is consistent with experimental results, which verifies the atomic model.Another combination of metals, 1.33 ML of Pb and 0.85 ML of In, resulted in the formation of a well-defined In/Pb/Ge(111)3×3 surface alloy. The 3×3 surface exhibits an interesting band structure where five surface bands were identified of which four cross the Fermi level resulting in a metallic character of the surface. Two-dimensional constant energy data reveal the presence of intricate rotated hexagon like contours which intersect each other along the − and − directions of the surface Brillouin zone. The STM results reveal nine bright protrusions per 3×3 unit cell.
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