| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Li, G. H., et al.
(författare)
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Surface element segregation and electrical conductivity of lithium layered transition-metal oxide cathode materials
- 2018
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Ingår i: Applied Surface Science. - : Elsevier BV. - 0169-4332. ; 427, s. 226-232
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Tidskriftsartikel (refereegranskat)abstract
- Surface element segregation and electric conductivity are critical in determining lithium storage ability of given cathode materials, which are poorly understood and not correlated with the structure and overall performance. Here, layered lithium transition-metal oxides, one of the state-of-the-art cathode materials for lithium ion batteries are chosen to study. A serial of LiNixCo1-2xMnxO2 samples were prepared via a solid state reaction and subsequently characterized by XRD in conjunction with structural refinement, XPS depth profiling, and AC impedance spectroscopy. Slightly different expansion rates are observed for lattice parameters (a and c/3) with varying of Ni content, which is attributed to the increase of average metalion radius and an increase of e(g) electron that enhances the columbic repulsion between transition metal and oxygen atoms. XPS depth profiling results show that surface composition is significantly deviated from bulk, in which Ni and Mn atoms tend to enrich in the surface region, while Co element is relatively deficient. Further, surface element segregation is alleviated by the increase of Ni/Mn content. Moreover, increasing the Ni/Mn content also raises the activation energy of bulk conduction.
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| 3. |
- Abel, I, et al.
(författare)
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Overview of the JET results with the ITER-like wall
- 2013
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Ingår i: Nuclear Fusion. - : IOP Publishing. - 1741-4326 .- 0029-5515. ; 53:10, s. 104002-
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Tidskriftsartikel (refereegranskat)abstract
- Following the completion in May 2011 of the shutdown for the installation of the beryllium wall and the tungsten divertor, the first set of JET campaigns have addressed the investigation of the retention properties and the development of operational scenarios with the new plasma-facing materials. The large reduction in the carbon content (more than a factor ten) led to a much lower Z(eff) (1.2-1.4) during L- and H-mode plasmas, and radiation during the burn-through phase of the plasma initiation with the consequence that breakdown failures are almost absent. Gas balance experiments have shown that the fuel retention rate with the new wall is substantially reduced with respect to the C wall. The re-establishment of the baseline H-mode and hybrid scenarios compatible with the new wall has required an optimization of the control of metallic impurity sources and heat loads. Stable type-I ELMy H-mode regimes with H-98,H-y2 close to 1 and beta(N) similar to 1.6 have been achieved using gas injection. ELM frequency is a key factor for the control of the metallic impurity accumulation. Pedestal temperatures tend to be lower with the new wall, leading to reduced confinement, but nitrogen seeding restores high pedestal temperatures and confinement. Compared with the carbon wall, major disruptions with the new wall show a lower radiated power and a slower current quench. The higher heat loads on Be wall plasma-facing components due to lower radiation made the routine use of massive gas injection for disruption mitigation essential.
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| 4. |
- Ge, Chenjie, 1991, et al.
(författare)
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Co-saliency detection via similarity-based saliency propagation
- 2015
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Ingår i: 2015 IEEE International Conference on Image Processing (ICIP). ; , s. 1845 - 1849
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Konferensbidrag (refereegranskat)abstract
- In this paper, we present a method for discovering the common salient objects from a set of images. We treat co-saliency detection as a pairwise saliency propagation problem, which utilizes the similarity between each pair of images to measure the common property with the guidance of a single saliency map image. Given the pairwise co-salient foreground maps, pairwise saliency is optimized by combining the initial background cues. Pairwise co-salient maps are then fused according to a novel fusion strategy based on the focus of human attention. Finally we adopt an integrated multi-scale scheme to obtain the pixel-level saliency map. Our proposed model makes the existing single saliency model perform well in co-saliency detection and is not overly sensitive to the initial saliency model selected. Extensive experiments on two benchmark databases show the superiority of our co-saliency model against the state-of-the-art methods both subjectively and objectively.
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| 5. |
- Romanelli, F, et al.
(författare)
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Overview of the JET results
- 2011
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Ingår i: Nuclear Fusion. - : IOP Publishing. - 1741-4326 .- 0029-5515. ; 51:9
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Tidskriftsartikel (refereegranskat)abstract
- Since the last IAEA Conference JET has been in operation for one year with a programmatic focus on the qualification of ITER operating scenarios, the consolidation of ITER design choices and preparation for plasma operation with the ITER-like wall presently being installed in JET. Good progress has been achieved, including stationary ELMy H-mode operation at 4.5 MA. The high confinement hybrid scenario has been extended to high triangularity, lower ρ*and to pulse lengths comparable to the resistive time. The steady-state scenario has also been extended to lower ρ*and ν*and optimized to simultaneously achieve, under stationary conditions, ITER-like values of all other relevant normalized parameters. A dedicated helium campaign has allowed key aspects of plasma control and H-mode operation for the ITER non-activated phase to be evaluated. Effective sawtooth control by fast ions has been demonstrated with3He minority ICRH, a scenario with negligible minority current drive. Edge localized mode (ELM) control studies using external n = 1 and n = 2 perturbation fields have found a resonance effect in ELM frequency for specific q95values. Complete ELM suppression has, however, not been observed, even with an edge Chirikov parameter larger than 1. Pellet ELM pacing has been demonstrated and the minimum pellet size needed to trigger an ELM has been estimated. For both natural and mitigated ELMs a broadening of the divertor ELM-wetted area with increasing ELM size has been found. In disruption studies with massive gas injection up to 50% of the thermal energy could be radiated before, and 20% during, the thermal quench. Halo currents could be reduced by 60% and, using argon/deuterium and neon/deuterium gas mixtures, runaway electron generation could be avoided. Most objectives of the ITER-like ICRH antenna have been demonstrated; matching with closely packed straps, ELM resilience, scattering matrix arc detection and operation at high power density (6.2 MW m-2) and antenna strap voltages (42 kV). Coupling measurements are in very good agreement with TOPICA modelling. © 2011 IAEA, Vienna.
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| 6. |
- Cao, Wei, et al.
(författare)
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Interaction With Social Robots : Improving Gaze Toward Face but Not Necessarily Joint Attention in Children With Autism Spectrum Disorder
- 2019
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Ingår i: Frontiers in Psychology. - : FRONTIERS MEDIA SA. - 1664-1078. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- It is widely recognized that robot-based interventions for autism spectrum disorders (ASD) hold promise, but the question remains as to whether social humanoid robots could facilitate joint attention performance in children with ASD. In this study, responsive joint attention was measured under two conditions in which different agents, a human and a robot, initiated joint attention via video. The participants were 15 children with ASD (mean age: 4.96 +/- 1.10 years) and 15 typically developing (TD) children (mean age: 4.53 +/- 0.90 years). In addition to analyses of fixation time and gaze transitions, a longest common subsequence approach (LCS) was employed to compare participants' eye movements to a predefined logical reference sequence. The fixation of TD toward agent's face was earlier and longer than children with ASD. Moreover, TD showed a greater number of gaze transitions between agent's face and target, and higher LCS scores than children with ASD. Both groups showed more interests in the robot's face, but the robot induced a lower proportion of fixation time on the target. Meanwhile participants showed similar gaze transitions and LCS results in both conditions, suggesting that they could follow the logic of the joint attention task induced by the robot as well as human. We have discussed the implications for the effects and applications of social humanoid robots in joint attention interventions.
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| 7. |
- Ge, Chenjie, 1991, et al.
(författare)
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Co-saliency detection via inter and intra saliency propagation
- 2016
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Ingår i: Signal Processing: Image Communication. - 0923-5965. ; 44, s. 69-83
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Tidskriftsartikel (refereegranskat)abstract
- The goal of salient object detection from an image is to extract the regions which capture the attention of the human visual system more than other regions of the image. In this paper a novel method is presented for detecting salient objects from a set of images, known as co-saliency detection. We treat co-saliency detection as a two-stage saliency propagation problem. The first inter-saliency propagation stage utilizes the similarity between a pair of images to discover common properties of the images with the help of a single image saliency map. With the pairwise co-salient foreground cue maps obtained, the second intra-saliency propagation stage refines pairwise saliency detection using a graph-based method combining both foreground and background cues. A new fusion strategy is then used to obtain the co-saliency detection results. Finally an integrated multi-scale scheme is employed to obtain pixel-level co-saliency maps. The proposed method makes use of existing saliency detection models for co-saliency detection and is not overly sensitive to the initial saliency model selected. Extensive experiments on three benchmark databases show the superiority of the proposed co-saliency model against the state-of-the-art methods both subjectively and objectively.
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| 8. |
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| 9. |
- Yin, S., et al.
(författare)
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Long-term precipitation change by hourly data in Haihe River Basin during 1961-2004
- 2011
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Ingår i: SCIENCE CHINA Earth Sciences. - : Springer Science and Business Media LLC. - 1674-7313 .- 1869-1897. ; 54:10, s. 1576-1585
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Tidskriftsartikel (refereegranskat)abstract
- Hourly summer precipitation data recorded at 21 stations during 1961–2004 in the Haihe River Basin in North China were analyzed. The results show that the precipitation frequency and amount and the morning peak mainly relating to long-duration rainfall events decreased during this period, whereas the normalized afternoon peak mainly relating to short-duration events increased, which may suggest that the proportion of short-duration rainfall has increased as the total summer rainfall has decreased. For short-duration events, the mean intensity and peak intensity increased at most stations and the time to peak intensity decreased, which may be attributable to the higher thermal contrast between the warmer lower surface and cooler upper level. In the case of long-duration events, the total amount was significantly correlated with the East Asian summer monsoon index for the period 1961–2001 (correlation coefficient of 0.63). Although the total amount of rainfall in long-duration events decreased in the basin, the mean intensity and peak intensity, as well as the extreme hourly precipitation, increased in the western basin and decreased in the eastern basin.
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