| 1. |
- Li, Shiyu, 1991-
(författare)
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Engineering Surfaces of Solid-State Nanopores for Biomolecule Sensing
- 2021
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Konstnärligt arbete (övrigt vetenskapligt/konstnärligt)abstract
- Nanopores have emerged as a special class of single-molecule analytical tool that offers immense potential for sensing and characterizing biomolecules such as nucleic acids and proteins. As an alternative to biological nanopores, solid-state nanopores present remarkable versatility due to their wide-range tunability in pore geometry and dimension as well as their excellent mechanical robustness and stability. However, being intrinsically incompatible with biomolecules, surfaces of inorganic solids need be modified to provide desired functionalities for real-life sensing purposes. In this thesis, we presented an exploration of various surface engineering strategies and an examination of several surface associated phenomena pertaining specifically to solid-state nanopores. Based on the parallel sensing concept using arrayed pores, optical readout is mainly employed throughout the whole study.For the surface engineering aspect, a list of approaches was explored. A versatile surface patterning strategy for immobilization of biomolecules was developed based on selective poly(vinylphosphonic acid) passivation and electron beam induced deposition technique. This scheme was then implemented on nanopore arrays for nanoparticle localization. In addition, vesicle rupture-based lipid bilayer coating was adapted to truncated-pyramidal nanopores, which was shown to be effective for the minimizing DNA-pore interaction. Further, HfO2 coating by means of atomic layer deposition was employed to prevent the erosion of Si-based pores and to shrink the pore diameter, which enabled reliable investigations of DNA clogging and DNA polymerase docking.For the surface associated phenomena, several findings were made. The lipid bilayer formation on truncated pyramidal nanopores via instantaneous rupture of individual vesicles was quantified based on combined ionic current monitoring and optical observation. The probability of pore clogging appeared to linearly increase with the length of DNA strands and applied bias voltage, which could be attributed a higher probability of knotting and/or folding of longer DNA strands and more frequent translocation events at higher voltage. A free-energy based analytical model was proposed to evaluate the DNA-pore interaction and to interpret observed clogging behavior. Finally, docking of DNA polymerase on nanopore arrays was demonstrated using label-free optical method based on Ca2+ indicator dyes, which may open the avenue to sequencing-by-synthesis enabled by the docked polymerase.
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| 2. |
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Bergtagen
- 2020. - 200
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Samlingsverk (redaktörskap) (populärvet., debatt m.m.)
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| 3. |
- Cheng, Xiaogang, et al.
(författare)
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NIDL: A pilot study of contactless measurement of skin temperature for intelligent building
- 2019
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Ingår i: Energy and Buildings. - : Elsevier. - 0378-7788 .- 1872-6178. ; 198, s. 340-352
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Tidskriftsartikel (refereegranskat)abstract
- Human thermal comfort measurement plays a critical role in giving feedback signals for building energy efficiency. A contactless measuring method based on subtleness magnification and deep learning (NIDL) was designed to achieve a comfortable, energy efficient built environment. The method relies on skin feature data, e.g., subtle motion and texture variation, and a 315-layer deep neural network for constructing the relationship between skin features and skin temperature. A physiological experiment was conducted for collecting feature data (1.44 million) and algorithm validation. The contactless measurement algorithm based on a partly-personalized saturation temperature model (NIPST) was used for algorithm performance comparisons. The results show that the mean error and median error of the NIDL are 0.476 °C and 0.343°C which is equivalent to accuracy improvements of 39.07 % and 38.76 %, respectively.
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| 4. |
- Guo, Li, et al.
(författare)
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Electroconductive textiles and textile-based electromechanical sensors — integration in as an approach for smart textiles
- 2016. - 1
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Ingår i: Smart Textiles and their Applications. - : Woodhead Publishing Limited. - 9780081005743 ; , s. 657-693
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Bokkapitel (refereegranskat)abstract
- The unification of textiles and electrics opens up many interesting possibilities for sensorics, actuation, energy transport, energy storage, and information transport. Electrics need conductive structures. Industrially knittable and weavable filaments and yarns are in this chapter overviewed in a typology of seven classes. These are the basics for the integration in approach that is put forward as a concept for successful production of smart textiles.Integration means that a "device" is (1) made by a textile production process and (2) made as a textile. We focus on smart textiles for mechanical sensoring that give an electrical output as these embrace such basic quantities as position, movement, speed, acceleration, elongation, forces, pressure, and vibration. Cases of mechanical sensors are demonstrated based on piezoelectricity and capacitive techniques. It is shown that these are promising technologies for smart textiles in general and the integration approach specifically.
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| 5. |
- Jing, Li, et al.
(författare)
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In-situ AFM and EIS study of a solventborne alkyd coating with nanoclay for corrosion protection of carbon steel
- 2015
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Ingår i: Progress in organic coatings. - : Elsevier BV. - 0300-9440 .- 1873-331X. ; 87, s. 179-188
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Tidskriftsartikel (refereegranskat)abstract
- A solventborne alkyd composite coating containing modified montmorillonite (MMT) nanoclay was made on carbon steel, and its corrosion protection was investigated by in-situ atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS) measurements in 3 wt.% NaCl solution. X-ray diffraction (XRD) analysis indicated intercalation of the MMT sheets in the composite coating. Thermo-gravimetric analysis (TGA) demonstrated improved thermal stability of the composite coating due to the modified nanoclay. Scanning electron microscopy (SEM) and AFM examination revealed dispersion and also some aggregation of the nanoclay particles in the coating. In-situ AFM images show a stable coating surface at nano-scale during relative long time exposure in the NaCl solution, indicating an enhanced stability of the composite coating. The EIS results confirmed that the composite coating provides an enhanced barrier type corrosion protection for carbon steel in the corrosive solution, which could be attributed to the intercalated lamellar MMT sheets in the coating that block the defects and decrease the transport of water and corrosive species.
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| 6. |
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| 7. |
- Jönsson, Li, et al.
(författare)
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The thickening of futures
- 2021
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Ingår i: Futures. - : Elsevier. - 0016-3287 .- 1873-6378. ; 134:December
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Tidskriftsartikel (refereegranskat)abstract
- This paper engages with biodiversity loss. In particular, it focuses on observations and scientific facts: the decline of pollinators and what that entails for the co-living of humans and more-than-humans. This kind of work often reaches the publics as thin stories of limited futures.The article explores how to situate the issue of out-of-sync plant–pollinator relationships into thick, ongoing presents rather than as a distant future that is out of one’s own hands. This is done through a collaborative design project that experiments with various formats for staging more material, embodied and experiential ways to sensitise and invite humans to experience the issue of pollination. We therefore explore and give an account of how we have situated the issues in a thick, ongoing present as an anticipatory practice. We thus suggest a practice that becomes both sticky and sweaty; in addition, the practice moves some pollination facts into not only matters of concern but also matters of care.In doing so, we forward the role that design researchers can play in environmental and collaborative anticipation by engaging with emerging approaches to both biodiversity loss and collaborative future-making that are simultaneously conflicting and harsh as well as hopeful.
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