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Marmoset glutathion...
Marmoset glutathione transferases with ketosteroid isomerase activity
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- Ismail, Aram (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Sawmi, Julia (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Mannervik, Bengt (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- English.
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In: Biochemistry and Biophysics Reports. - : Elsevier BV. - 2405-5808. ; 27
- Related links:
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- The common marmoset Callithrix jacchus encodes two glutathione transferase (GST) enzymes with ketosteroid double-bond isomerase activity. The most active enzyme is CjaGST A3-3 showing a specific activity with 5-androsten-3,17-dione (Delta(5)-AD) of 62.1 +/- 1.8 mu mol min(-1) mg(-1), and a k(cat) value of 261 +/- 49 s(-1). The second ketostemid isomerase CjaGST A1-1 has a 30-fold lower specific activity with Delta(5)-AD and a 37-fold lower k(cat) value. Thus, the marmoset CjaGST A3-3 would be the main contributor to the biosynthesis of the steroid hormones testosterone and progesterone, like the human ortholog HsaGST A3-3. Two residues differ in the H-site of the 91.4% sequence identical CjaGST A1-1 and CjaGST A3-3, and modeling of the structures suggests that the bulky phenyl ring of Phe111 in CjaGST A1-1 causes steric hindrance in the binding of the steroid substrate. Tributyltin acetate (IC50 =0.16 +/- 0.004 mu M) and ethacrynic acid (IC50 =3.3 +/- 0.2 mu M) were found to be potent inhibitors of CjaGST A3-3, as previously demonstrated with the human and equine orthologs.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- 5-Androsten-3
- 17-dione
- 5-Pregnen-3
- 20-dione
- CjaGST A3-3
- CjaGST A1-1
- Alpha glutathione transferase
- Steroid hormone synthesis
Publication and Content Type
- ref (subject category)
- art (subject category)
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