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1.
  • Akkoyun, S., et al. (författare)
  • AGATA - Advanced GAmma Tracking Array
  • 2012
  • Ingår i: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. - : Elsevier BV. - 0168-9002 .- 0167-5087 .- 1872-9576. ; 668, s. 26-58
  • Tidskriftsartikel (refereegranskat)abstract
    • The Advanced GAmma Tracking Array (AGATA) is a European project to develop and operate the next generation γ-ray spectrometer. AGATA is based on the technique of γ-ray energy tracking in electrically segmented high-purity germanium crystals. This technique requires the accurate determination of the energy, time and position of every interaction as a γ ray deposits its energy within the detector volume. Reconstruction of the full interaction path results in a detector with very high efficiency and excellent spectral response. The realisation of γ-ray tracking and AGATA is a result of many technical advances. These include the development of encapsulated highly segmented germanium detectors assembled in a triple cluster detector cryostat, an electronics system with fast digital sampling and a data acquisition system to process the data at a high rate. The full characterisation of the crystals was measured and compared with detector- response simulations. This enabled pulse-shape analysis algorithms, to extract energy, time and position, to be employed. In addition, tracking algorithms for event reconstruction were developed. The first phase of AGATA is now complete and operational in its first physics campaign. In the future AGATA will be moved between laboratories in Europe and operated in a series of campaigns to take advantage of the different beams and facilities available to maximise its science output. The paper reviews all the achievements made in the AGATA project including all the necessary infrastructure to operate and support the spectrometer. © 2011 Elsevier B.V. All rights reserved.
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2.
  • Gandla, Madhavi Latha, et al. (författare)
  • Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees
  • 2021
  • Ingår i: Biotechnology for Biofuels. - : Springer Science and Business Media LLC. - 1754-6834. ; 14:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Background Bioconversion of wood into bioproducts and biofuels is hindered by the recalcitrance of woody raw material to bioprocesses such as enzymatic saccharification. Targeted modification of the chemical composition of the feedstock can improve saccharification but this gain is often abrogated by concomitant reduction in tree growth. Results In this study, we report on transgenic hybrid aspen (Populus tremula x tremuloides) lines that showed potential to increase biomass production both in the greenhouse and after 5 years of growth in the field. The transgenic lines carried an overexpression construct for Populus tremula x tremuloides vesicle-associated membrane protein (VAMP)-associated protein PttVAP27-17 that was selected from a gene-mining program for novel regulators of wood formation. Analytical-scale enzymatic saccharification without any pretreatment revealed for all greenhouse-grown transgenic lines, compared to the wild type, a 20-44% increase in the glucose yield per dry weight after enzymatic saccharification, even though it was statistically significant only for one line. The glucose yield after enzymatic saccharification with a prior hydrothermal pretreatment step with sulfuric acid was not increased in the greenhouse-grown transgenic trees on a dry-weight basis, but increased by 26-50% when calculated on a whole biomass basis in comparison to the wild-type control. Tendencies to increased glucose yields by up to 24% were present on a whole tree biomass basis after acidic pretreatment and enzymatic saccharification also in the transgenic trees grown for 5 years on the field when compared to the wild-type control. Conclusions The results demonstrate the usefulness of gene-mining programs to identify novel genes with the potential to improve biofuel production in tree biotechnology programs. Furthermore, multi-omic analyses, including transcriptomic, proteomic and metabolomic analyses, performed here provide a toolbox for future studies on the function of VAP27 proteins in plants.
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3.
  • Knight, R, et al. (författare)
  • GTPOM: Thermo-economic optimization of whole gas turbine plant
  • 2006
  • Ingår i: Journal of Engineering for Gas Turbines and Power. - : ASME International. - 1528-8919 .- 0742-4795. ; 128:3, s. 535-542
  • Tidskriftsartikel (refereegranskat)abstract
    • Trends towards distributed power generation and the deregulation of energy markets are increasing the requirement for software tools that optimize power generation plant design and operation. In this context, this paper describes the GTPOM (thermo-economic optimization of whole gas turbine plant) European project, funded in part through the European Commissions 5th Framework Programme, focusing on the development and demonstration of an original software tool for the thermo-economic analysis and optimization of conventional and advanced energy systems based on gas turbine plant. PSEconomy, the software tool developed during the GTPOM project, provides a thermo-economic optimization capability for advanced and more-conventional energy systems, enabling the complex trade-offs between system performance and installed costs to be determined for different operational duties and market scenarios. Furthermore, the code is capable of determining the potential benefits of innovative cycles or layout modifications to existing plants compared with current plant configurations. The economic assessment is performed through a complete through-life cycle cost analysis, which includes the total capital cost of the plant, the cost of fuel, O&M costs and the expected revenues from the sale of power and heat. The optimization process, carried out with a GA-based algorithm, is able to pursue different objective functions as specified by the User. These include system efficiency, through-life cost of electricity and through-life internal rate of return. Three case studies demonstrating the capabilities of the new tool are presented in this paper covering a conventional combined cycle system, a biomass plant and a CO2 sequestration gas turbine cycle. The software code is now commercially available and is expected to provide significant advantages in the near and long-term development of energy cycles.
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4.
  • Maury, J., et al. (författare)
  • Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae
  • 2008
  • Ingår i: FEBS Letters. - : Wiley. - 1873-3468 .- 0014-5793. ; 582:29, s. 4032-4038
  • Tidskriftsartikel (refereegranskat)abstract
    • A eukaryotic mevalonate pathway transferred and expressed in Escherichia coli, and a mammalian hydrocortisone biosynthetic pathway rebuilt in Saccharomyces cerevisiae are examples showing that transferring metabolic pathways from one organism to another can have a powerful impact on cell properties. In this study, we reconstructed the E. coli isoprenoid biosynthetic pathway in S. cerevisiae. Genes encoding the seven enzymatic steps of the pathway were cloned and expressed in S. cerevisiae. mRNA from the seven genes was detected, and the pathway was shown able to sustain growth of yeast in conditions of inhibition of its constitutive isoprenoid biosynthetic pathway. © 2008 Federation of European Biochemical Societies.
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