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Astrocytes have the...
Astrocytes have the capacity to act as antigen-presenting cells in the Parkinson's disease brain
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- Rostami, Jinar (författare)
- Uppsala universitet,Geriatrik
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- Fotaki, Grammatiki (författare)
- Uppsala universitet,Klinisk immunologi
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- Sirois, Julien (författare)
- Neuroimmunology Unit, Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada
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- Mzezewa, Ropafadzo (författare)
- Uppsala universitet,Geriatrik
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- Bergström, Joakim (författare)
- Uppsala universitet,Geriatrik
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- Essand, Magnus (författare)
- Uppsala universitet,Klinisk immunologi
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- Healy, Luke (författare)
- Neuroimmunology Unit, Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada
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- Erlandsson, Anna (författare)
- Uppsala universitet,Geriatrik
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(creator_code:org_t)
- 2020-04-16
- 2020
- Engelska.
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Ingår i: Journal of Neuroinflammation. - : Springer Science and Business Media LLC. - 1742-2094. ; 17:1
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://jneuroinflam...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- BackgroundMany lines of evidence suggest that accumulation of aggregated alpha-synuclein (αSYN) in the Parkinson’s disease (PD) brain causes infiltration of T cells. However, in which ways the stationary brain cells interact with the T cells remain elusive. Here, we identify astrocytes as potential antigen-presenting cells capable of activating T cells in the PD brain. Astrocytes are a major component of the nervous system, and accumulating data indicate that astrocytes can play a central role during PD progression.MethodsTo investigate the role of astrocytes in antigen presentation and T-cell activation in the PD brain, we analyzed post mortem brain tissue from PD patients and controls. Moreover, we studied the capacity of cultured human astrocytes and adult human microglia to act as professional antigen-presenting cells following exposure to preformed αSYN fibrils.ResultsOur analysis of post mortem brain tissue demonstrated that PD patients express high levels of MHC-II, which correlated with the load of pathological, phosphorylated αSYN. Interestingly, a very high proportion of the MHC-II co-localized with astrocytic markers. Importantly, we found both perivascular and infiltrated CD4+ T cells to be surrounded by MHC-II expressing astrocytes, confirming an astrocyte T cell cross-talk in the PD brain. Moreover, we showed that αSYN accumulation in cultured human astrocytes triggered surface expression of co-stimulatory molecules critical for T-cell activation, while cultured human microglia displayed very poor antigen presentation capacity. Notably, intercellular transfer of αSYN/MHC-II deposits occurred between astrocytes via tunneling nanotubes, indicating spreading of inflammation in addition to toxic protein aggregates.ConclusionsIn conclusion, our data from histology and cell culture studies suggest an important role for astrocytes in antigen presentation and T-cell activation in the PD brain, highlighting astrocytes as a promising therapeutic target in the context of chronic inflammation.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Alpha-synuclein
- Antigen presentation
- Astrocytes
- MHC-II
- Parkinson’s disease
- T-cell infiltration
- Tunneling nanotubes
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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