| 1. |
- Andersson, Mattias K, 1979, et al.
(författare)
-
The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response
- 2008
-
Ingår i: BMC Cell Biology. - : Springer Science and Business Media LLC. - 1471-2121. ; 9
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: FUS, EWS and TAF15 are structurally similar multifunctional proteins that were first discovered upon characterization of fusion oncogenes in human sarcomas and leukemias. The proteins belong to the FET (previously TET) family of RNA-binding proteins and are implicated in central cellular processes such as regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. In the present study, we investigated the expression and cellular localization of FET proteins in multiple human tissues and cell types. RESULTS: FUS, EWS and TAF15 were expressed in both distinct and overlapping patterns in human tissues. The three proteins showed almost ubiquitous nuclear expression and FUS and TAF15 were in addition present in the cytoplasm of most cell types. Cytoplasmic EWS was more rarely detected and seen mainly in secretory cell types. Furthermore, FET expression was downregulated in differentiating human embryonic stem cells, during induced differentiation of neuroblastoma cells and absent in terminally differentiated melanocytes and cardiac muscle cells. The FET proteins were targeted to stress granules induced by heat shock and oxidative stress and FUS required its RNA-binding domain for this translocation. Furthermore, FUS and TAF15 were detected in spreading initiation centers of adhering cells. CONCLUSION: Our results point to cell-specific expression patterns and functions of the FET proteins rather than the housekeeping roles inferred from earlier studies. The localization of FET proteins to stress granules suggests activities in translational regulation during stress conditions. Roles in central processes such as stress response, translational control and adhesion may explain the FET proteins frequent involvement in human cancer.
|
|
| 2. |
- Jonnergård, Karin, 1957-, et al.
(författare)
-
Information environment : an exploration and clarification of the concept based on prior literature
- 2020
-
Ingår i: Advances in Accounting. - : Elsevier. - 0882-6110. ; 50, s. 1-11
-
Tidskriftsartikel (refereegranskat)abstract
- In the accounting and finance literature, the concept of the information environment (IE) is widely used, but its meaning varies across different articles. Given the considerable presence of the IE concept, it seems relevant to clarify the concept and allow a clearer path forward for future research. With this ambition, this paper reviews articles that apply the concept of IE. Our sample consists of 248 articles published within the accounting and finance field between 2000 and 2017. We confirm an increasing trend in the use of the concept over time and discuss the lack of distinctness in the concept's general meaning. Several definitions of IE appear parallel to one another in the literature; in many cases, these definitions are also inconsistent. To analyze the concept, we use a two-step approach with the following subordinated aims: 1) to identify and clarify different types of IE, and 2) to identify and clarify the components that constitute the firm's IE. We find that the description preceding the term IE signals a border between different types of information or between different societal levels of IE. Also, the concept appears as a “system concept,” and its inclusion of different actors, the relations among these actors, and the effect of these relations on information asymmetry are core aspects that need to be described in future research.
|
|
| 3. |
- Karakaya, Sinan, et al.
(författare)
-
Cytoplasmic HIF-2α as tissue biomarker to identify metastatic sympathetic paraganglioma
- 2023
-
Ingår i: Scientific Reports. - 2045-2322. ; 13:1
-
Tidskriftsartikel (refereegranskat)abstract
- Pheochromocytomas (PCCs) and paragangliomas (PGLs) are rare neuroendocrine tumors. PGLs can further be divided into sympathetic (sPGLs) and head-and-neck (HN-PGLs). There are virtually no treatment options, and no cure, for metastatic PCCs and PGLs (PPGLs). Here, we composed a tissue microarray (TMA) consisting of 149 PPGLs, reflecting clinical features, presenting as a useful resource. Mutations in the pseudohypoxic marker HIF-2 & alpha; correlate to an aggressive tumor phenotype. We show that HIF-2 & alpha; localized to the cytoplasm in PPGLs. This subcompartmentalized protein expression differed between tumor subtypes, and strongly correlated to proliferation. Half of all sPGLs were metastatic at time of diagnosis. Cytoplasmic HIF-2 & alpha; was strongly expressed in metastatic sPGLs and predicted poor outcome in this subgroup. We propose that higher cytoplasmic HIF-2 & alpha; expression could serve as a useful clinical marker to differentiate paragangliomas from pheochromocytomas, and may help predict outcome in sPGL patients.
|
|
