| 1. |
- Nilsson, E.J.C., et al.
(författare)
-
Using microdispensing to manufacture a customized cell dish for microbeam irradiation of single, living cells
- 2009
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms. - : Elsevier BV. - 0168-583X. ; 267:7, s. 1199-1205
-
Tidskriftsartikel (refereegranskat)abstract
- In this paper is described the preparation of patterned cell dishes to be used in studies of low dose irradiation effects on living cells. Using a droplet microdispenser, an 8 mu m thick polypropylene cell substrate, to which cells do not naturally adhere, was coated in a matrix pattern with the cell adhesive mussel protein Cell-Tak. Cells were shown to adhere and grow on the protein-coated spots, but not on the uncoated parts, providing for guided cell growth. Cultivation of isolated cell colonies provides an opportunity to study how low doses of ionizing radiation affect neighbouring un-irradiated cell colonies. (C) 2009 Elsevier B.V. All rights reserved.
|
|
| 2. |
- Nilsson, Charlotta, et al.
(författare)
-
Characterisation of a pre-cell hit detector to be used in single cell irradiation experiments at the Lund Nuclear Microprobe
- 2008
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms. - Amsterdam : Elsevier BV. - 0168-583X .- 1872-9584. ; 266:21, s. 4808-4815
-
Tidskriftsartikel (refereegranskat)abstract
- This paper describes the characterisation of an ultra-thin silicon semicondoctor Delta E detector to be used as a pre-cell ion hit detector in single ion experiments on individual, living cells. The characteristics of interest for this specific application are the hit detection efficiency, which has to be close to 100% to enable bombardment with either a single ion or a counted number of ions, the beam spreading, which should be as small as possible to maintain the targeting accuracy, and the vacuum tightness, since the detector is intended, if possible, to be used simultaneously as vacuum window. The hit detection efficiency was shown to be above 99% when detecting alpha particles or 2 MeV protons, the increase in beam size was about 1 mu m and the vacuum tightness was comparable to that of the Si3N4 wafer which is normally used as vacuum window, thus the Delta E detector fulfils the main criteria to function properly as a single ion hit detector. (C) 2008 Elsevier B.V. All rights reserved.
|
|
| 3. |
- Nilsson, Charlotta, et al.
(författare)
-
Evaluation of a pre-cell hit detector for the future single ion hit facility in Lund
- 2006
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms. - : Elsevier BV. - 0168-583X .- 1872-9584. ; 249:1-2, s. 924-927
-
Tidskriftsartikel (refereegranskat)abstract
- Currently, a single ion hit facility, for irradiation of single cells with single, light MeV ions is under development at the Lund Nuclear Microprobe. In this paper, a novel approach to the ion detection issue is presented. A silicon detector, a type utilized at other facilities for post-cell ion detection, has been investigated as a possible option for pre-cell hit detection. If proven successful, this detector could possibly also be used simultaneously as vacuum window. The first experiments carried out on the 9 mu m thick silicon detector, with an area of 4 mm(2), have been aimed at investigations of signal-to-noise ratio and efficiency. The results thus far reveal a low noise level and a noise distribution, which is well separated from the signal peak. However, the efficiency remains a problem, since at present it is far from the required 100%.
|
|
| 4. |
- Pallon, Jan, et al.
(författare)
-
At the Tip of an MeV Beam: Provoking Cells and Performing Tomographic Imaging
- 2009
-
Ingår i: Acta Physica Polonica A. - 0587-4246. ; 115:2, s. 501-506
-
Konferensbidrag (refereegranskat)abstract
- Biological applications of ion beams have recently become a new important research field using single ion hit facilities to study individual living cells and their response to the hit of a counted number of ions. One motivation is the search for a better understanding of the fundamental processes taking place in cells and organs as a result of irradiation. Another comes from the increasing interest in using high energy protons and heavy ions as a modality for radiotherapy of deep seated tumours. In the view of treatment efficiency, study of cell culture behaviour under controlled radiation experiments, and in different chemical environments at single ion hit facilities, is a first step towards a better understanding of the processes. Tomographic techniques are applicable to situations where you need information of the inside of an object but do not want to section it into thin slices or cannot do it. Using focused MeV ion beams for tomography restricts the sample size to the order of 10-100 mu m, depending of the initial energy. On the other hand, the ability to focus at a sub-micrometer level makes ion beams well suited for analyses of small sized objects as cells, spores, etc. The scanning transmission ion microscopy mode of tomography gives the mass density and corresponding morphological structure of holes and pores. It can then be used to correct the results from the other mode, particle induced X-ray emission tomography. Here is discussed a porosity analysis of bentonite clay that is planned to form an important buffer zone around canisters filled with spent nuclear reactor fuel waste deposited 500 m underground in Sweden.
|
|
| 5. |
- Wegdén, Marie, et al.
(författare)
-
Nuclear microprobe analysis of the selective boron uptake obtained with BPA in brain tumour tissue
- 2004
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms. - : Elsevier BV. - 0168-583X. ; 219-20, s. 67-71
-
Tidskriftsartikel (refereegranskat)abstract
- The tumour selective ability of the boron compound boronophenylalanine (BPA), today used in Boron Neutron Capture Therapy in Sweden, has been investigated with the Lund Nuclear Microprobe. The tumour to tissue ratio of the boron concentration, as well as the location of boron within the cells, is critical for the efficiency of the therapy. It is desirable that the boron is accumulated as close as possible to the cell nucleus, since the alpha particles produced in the B-10(n,alpha)Li-7 reaction only have a range of about 10 microns, i.e. a cell diameter. The nuclear reaction B-11(p,alpha)2alpha, which has an especially high cross-section (300 mb) for 660 keV protons, has been used to analyse brain tissue from BPA-injected rats. Previous studies on other boron compounds have shown significant background problems when the alpha particles are detected in the backward direction. By a specially designed set-up, alpha particles in the forward and backward direction are detected simultaneously, and only the coincidences between the two directions are considered to be true boron events. In this way we could achieve excellent background suppression. The analysis shows that BPA indeed is tumour selective. Quantifications show a boron abundance of 150 +/- 20 ng/cm(2) in normal tissue and 567 70 ng/cm(2) in tumour tissue. If the rat is fed with L-dopa before the injection of BPA the uptake increases 3-4 times. The boron is homogeneously distributed in the cellular structure and no specific intracellular accumulation has been shown. (C) 2004 Elsevier B.V. All rights reserved.
|
|
| 6. |
- Abdel, Naseem S., et al.
(författare)
-
Characterizations of new Delta E detectors for single-ion hit facility
- 2014
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms. - : Elsevier BV. - 0168-583X. ; 318, s. 281-286
-
Tidskriftsartikel (refereegranskat)abstract
- This paper describes the performance evaluation of new Delta E detectors to be used as pre-cell hit detectors in living cell irradiation experiments at the Lund Ion Beam Analysis facility (LIBAF). Using these detectors with a thickness down to 4.15 mu m fabricated at Lund University, an experiment was setup in which Delta E-detectors were used together with a stop E-detector in a telescope system under coincidence measurements. The characteristics of Delta E detectors were based on the optimal detection of the passage of 2.55 MeV protons. The results of these tests demonstrate that the detector telescope clearly separates the protons, this due to high signal-to-noise ratio and good energy resolution of the Delta E-detectors. The best performing detector was shown to have a detection efficiency of 95% at thickness of 9.7 mu m. This type of high-performing detector is suitable for the planned role of the Delta E-detector in the future cell irradiation experiments. (C) 2013 Elsevier B.V. All rights reserved.
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
- Ahlqvist, Kerstin, et al.
(författare)
-
The Association of Self-Reported Generalized Joint Hypermobility with pelvic girdle pain during pregnancy : a retrospective cohort study
- 2020
-
Ingår i: BMC Musculoskeletal Disorders. - : BMC. - 1471-2474. ; 21:1
-
Tidskriftsartikel (refereegranskat)abstract
- Background Pelvic girdle pain (PGP) is common during pregnancy but the causes remain poorly understood. Generalized joint hypermobility (GJH) is an inherited trait, with joint mobility beyond normal limits and is assumed to be related with PGP. The aim of this project was to study the association between self-reported GJH and the presence of PGP during pregnancy. Methods In this cohort study, 4884 Swedish-speaking women were consecutively recruited at their first visit for registration in the national antenatal screening programme in Sweden. We used the five-part questionnaire (5PQ) to assess GJH and pain drawings to identify PGP. Our primary outcome was the presence of PGP during the entire pregnancy and secondary outcomes were PGP in each trimesters. We tested the associations with logistic regression analysis, and adjusted for age and ethnicity. Results In all,2455 (50.3%) women responded to both questionnaires. The prevalence of self-reported GJH was 28.7%. A higher proportion of women with GJH than women without GJH reported PGP during the entire pregnancy (47.9% vs. 41.0%), particularly in trimester 1 (31.6% vs. 22.0%). Thus, women with GJH also had higher odds of PGP during the entire pregnancy (adjusted odds ratio (aOR) 1.27: 95% CI 1.11-1.47) and in trimester 1 (aOR 1.54: 95% CI 1.20-1.96), but the associations were not statistically significant in trimester 2 (aOR 1.24: 95% CI 0.82-1.88) or trimester 3 (aOR 1.20: 95% CI 0.99-1.45). The odds of PGP in pregnancy increased with increasing numbers of positive answers to the 5PQ (pfor linear trend < 0.001) for the entire pregnancy and in trimester 1 (pfor linear trend < 0.001), but not in trimesters 2 or 3 (p = 0.13 andp = 0.06, respectively). Conclusions Compared to women with normal joint mobility, women with GJH had higher odds of reporting PGP during pregnancy and the odds increased with number of positive responses to the 5PQ. The associations were present in trimester 1 but did not reach statistical significance in trimester 2 and 3.
|
|
| 10. |
- Arteaga, Natalia, et al.
(författare)
-
Applications of SU-8 in the development of a Single Ion Hit Facility
- 2009
-
Ingår i: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. - : Elsevier BV. - 0168-583X. ; 267:12-13, s. 2117-2121
-
Konferensbidrag (refereegranskat)abstract
- The Lund Nuclear Microprobe (LNM) has been adapted to be used as a Single Ion Hit Facility (SIHF) for proton cell irradiation experiments at low dose. In order to test the capabilities of the system, Ni dot arrays and artificial cells have been fabricated with the photopolymer SU-8 and common lithographic techniques. The primary purpose of the Ni dot arrays was to determine the targeting accuracy of the beam in vacuum and in air. Additionally, this sample was employed to evaluate the system performance during cell target irradiation experiments. The Ni dot arrays were also used for beam characterization. The artificial cells were originally fabricated to test the software for cell recognition and localization, developed and implemented at the LNM. However, this sample became very functional to make small adjustments at the irradiation chamber for cell irradiation experiments. A description of the samples, fabrication procedure and applications are presented in this paper. (C) 2009 Elsevier B.V. All rights reserved.
|
|
