| 1. |
- Welsh, Joshua A., et al.
(författare)
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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
- 2024
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Ingår i: Journal of Extracellular Vesicles. - : John Wiley and Sons Inc. - 2001-3078. ; 13:2
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Tidskriftsartikel (refereegranskat)abstract
- Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
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| 2. |
- Powell, John, et al.
(författare)
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The sensitivity of hybrid laser welding to variations in workpiece position
- 2011
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Ingår i: Physics Procedia. - : Elsevier BV. - 1875-3892. ; 12:1, s. 188-193
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Tidskriftsartikel (refereegranskat)abstract
- High speed imaging has been used to analyze the sensitivity of the Hybrid laser welding process to variations in the laser-arcworkpiece geometry along the axis of the laser beam. The welding process was found to be stable within a certain range of workpiece positions. Outside of this range the process became unstable. If the workpiece was too close to the laser/arc combination, the two energy sources did not supplement each other sufficiently. If the workpiece was too far away the droplets from the arc interfered with the laser-keyhole interaction.
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| 3. |
- Schneider, J., et al.
(författare)
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Additive manufacturing of a metallic optical bench—process development, material qualification and demonstration
- 2023
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Ingår i: CEAS Space Journal. - : Springer Nature. - 1868-2502 .- 1868-2510. ; 15:1, s. 55-68
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Tidskriftsartikel (refereegranskat)abstract
- With the large-class science mission ATHENA, the European Space Agency (ESA) aims at exploring the hot and energetic universe with advanced X-Ray technology. As a central component of the telescope, hundreds of silicon pore optic (SPO) modules will be assembled in an optical bench with a diameter of about 2.5 m. Several approaches are under investigation for the manufacturing of this supporting structure, and for handling the challenging constraints with respect to size, geometry and material. In cooperation with ESA, the Fraunhofer IWS is currently investigating the manufacturing of the optical bench made from Ti-6Al-4 V by means of Additive Manufacturing using Laser Metal Deposition (LMD) followed by subtractive finishing. Several development steps have been covered in a holistic manner starting with the system engineering of the production site. The main focus of the activity was on the process development for the Additive Manufacturing as well as the subtractive finishing. Furthermore, the properties of the produced material were also investigated. Within the scope of this publication, a general overview is given about the project related developments, achievements, and flanking activities for solving various challenges. The suitability of the developed technologies and workflows are now being evaluated through the manufacture of a representative, large-scale breadboard.
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