| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Kehoe, Laura, et al.
(författare)
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Make EU trade with Brazil sustainable
- 2019
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 364:6438, s. 341-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 3. |
- Birney, Ewan, et al.
(författare)
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Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project
- 2007
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 447:7146, s. 799-816
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Tidskriftsartikel (refereegranskat)abstract
- We report the generation and analysis of functional data from multiple, diverse experiments performed on a targeted 1% of the human genome as part of the pilot phase of the ENCODE Project. These data have been further integrated and augmented by a number of evolutionary and computational analyses. Together, our results advance the collective knowledge about human genome function in several major areas. First, our studies provide convincing evidence that the genome is pervasively transcribed, such that the majority of its bases can be found in primary transcripts, including non-protein-coding transcripts, and those that extensively overlap one another. Second, systematic examination of transcriptional regulation has yielded new understanding about transcription start sites, including their relationship to specific regulatory sequences and features of chromatin accessibility and histone modification. Third, a more sophisticated view of chromatin structure has emerged, including its inter-relationship with DNA replication and transcriptional regulation. Finally, integration of these new sources of information, in particular with respect to mammalian evolution based on inter- and intra-species sequence comparisons, has yielded new mechanistic and evolutionary insights concerning the functional landscape of the human genome. Together, these studies are defining a path for pursuit of a more comprehensive characterization of human genome function.
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| 4. |
- Bombarda, F., et al.
(författare)
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Runaway electron beam control
- 2019
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Ingår i: Plasma Physics and Controlled Fusion. - : IOP Publishing. - 1361-6587 .- 0741-3335. ; 61:1
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Tidskriftsartikel (refereegranskat)
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| 5. |
- Clausen Mork, Jonas, 1975-
(författare)
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Is it safe? safety factor reasoning in policy-making under uncertainty
- 2006
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis examines the practice of using safety factors in decision-making under uncertainty, in particular in the areas of toxicology and civil engineering. The aim is to expose and clarify some of the philosophical issues surrounding the practice. Paper I (co-written with Sven Ove Hansson and Fred Nilsson) gives an historical background to the practice of formal safety factor and safety margin use. The notion of an uncertainty function is presented as a more general concept covering safety margins, safety factors and the related uncertainty factors. Three categories of uncertainty functions are identified: explicit, implicit and natural safety reserves. Finally, the problems of countervailing risks and distribution arbitrariness are discussed. Paper II (co-written with John Cantwell) discusses the relation between decision-making with safety factor rules and the ideal of formal normative decision theory. The role of safety factor rules in practical and theoretical reasoning is also examined and certain difficulties regarding normative evaluation of safety factor rules are pointed out. Paper III (co-written with Sven Ove Hansson) looks at two current regulatory systems under development: Eurocodes for construction and REACH for chemicals. The two regulations have many similarities but reactions to them have been highly divergent. The differences are discussed and some hypotheses as to their explanation are suggested.
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| 6. |
- Fage-Pedersen, Jacob, et al.
(författare)
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Sn-background-induced diffusion enhancement of Sb in Si
- 1998
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Ingår i: Physical Review Letters. - 0031-9007. ; 81:26, s. 5856-5859
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Tidskriftsartikel (refereegranskat)abstract
- The diffusion of Sb in Si has been studied as a function of Sn-background concentration, and enhanced Sb diffusion is observed for backgrounds higher than CSn1 = 5 × 1019 cm-3. This concentration for the onset of enhanced diffusion is significantly lower than in other reports of high-concentration vacancy-mediated diffusion in Si. These reports, however, have up to now been concerned with donor impurities, whereas Sn is an electrically neutral impurity. Some Sn precipitation occurred, and the influence upon the diffusion is estimated from experiment to be small. A number of proposed models of high-concentration diffusion are discussed on the basis of the data.
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| 7. |
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- 2018
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Ingår i: Nuclear Fusion. - : IOP Publishing. - 1741-4326 .- 0029-5515. ; 58:1
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Forskningsöversikt (refereegranskat)
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