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Sökning: WFRF:(Pin Elisa) > Naturvetenskap

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1.
  • Fredolini, Claudia, et al. (författare)
  • Immunocapture strategies in translational proteomics
  • 2016
  • Ingår i: Expert Review of Proteomics. - : Taylor & Francis. - 1478-9450 .- 1744-8387. ; 13:1, s. 83-98
  • Forskningsöversikt (refereegranskat)abstract
    • Aiming at clinical studies of human diseases, antibody-assisted assays have been applied to biomarker discovery and toward a streamlined translation from patient profiling to assays supporting personalized treatments. In recent years, integrated strategies to couple and combine antibodies with mass spectrometry-based proteomic efforts have emerged, allowing for novel possibilities in basic and clinical research. Described in this review are some of the field's current and emerging immunocapture approaches from an affinity proteomics perspective. Discussed are some of their advantages, pitfalls and opportunities for the next phase in clinical and translational proteomics.
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2.
  • Pin, Elisa, et al. (författare)
  • Array-based profiling of proteins and autoantibody repertoires in CSF
  • 2019
  • Ingår i: Cerebrospinal Fluid (CSF) Proteomics. - New York, NY : Humana Press Inc.. ; , s. 303-318
  • Bokkapitel (refereegranskat)abstract
    • Protein profiling enabled through affinity proteomics represents a powerful strategy for analysis of complex samples such as human body fluids. Cerebrospinal fluid (CSF) is the proximal fluid of the central nervous system and is commonly analyzed in the context of neurological diseases. Through the presence of brain-derived proteins, this fluid can offer insight into the physiological state of the brain. Here, we describe multiplex and flexible protein and autoantibody profiling approaches using suspension bead arrays. Through minimal sample processing, these methods enable high-throughput analysis of hundreds of samples and proteins in one single assay and thereby provide powerful approaches for discovery of disease-associated proteins and autoantigens.
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