Array-based profiling of proteins and autoantibody repertoires in CSF

• Protein profiling enabled through affinity proteomics represents a powerful strategy for analysis of complex samples such as human body fluids. Cerebrospinal fluid (CSF) is the proximal fluid of the central nervous system and is commonly analyzed in the context of neurological diseases. Through the presence of brain-derived proteins, this fluid can offer insight into the physiological state of the brain. Here, we describe multiplex and flexible protein and autoantibody profiling approaches using suspension bead arrays. Through minimal sample processing, these methods enable high-throughput analysis of hundreds of samples and proteins in one single assay and thereby provide powerful approaches for discovery of disease-associated proteins and autoantigens.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

Affinity proteomics

Antibody arrays

Antigen arrays

Autoantibodies

Autoantibody profiling

Cerebrospinal fluid

Protein arrays

Protein profiling

autoantibody

protein

autoantigen

proteome

protein fingerprinting

brain

human

immunology

metabolism

procedures

protein microarray

proteomics

workflow

Autoantigens

Humans

Protein Array Analysis

Publication and Content Type

ref (subject category)

kap (subject category)