| 1. |
- Hudson, Thomas J., et al.
(författare)
-
International network of cancer genome projects
- 2010
-
Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 464:7291, s. 993-998
-
Tidskriftsartikel (refereegranskat)abstract
- The International Cancer Genome Consortium (ICGC) was launched to coordinate large-scale cancer genome studies in tumours from 50 different cancer types and/or subtypes that are of clinical and societal importance across the globe. Systematic studies of more than 25,000 cancer genomes at the genomic, epigenomic and transcriptomic levels will reveal the repertoire of oncogenic mutations, uncover traces of the mutagenic influences, define clinically relevant subtypes for prognosis and therapeutic management, and enable the development of new cancer therapies.
|
|
| 2. |
- Zhao, Li-Juan, et al.
(författare)
-
Lysine demethylase LSD1 delivered via small extracellular vesicles promotes gastric cancer cell stemness
- 2021
-
Ingår i: EMBO Reports. - : EMBO. - 1469-221X .- 1469-3178. ; 22:8
-
Tidskriftsartikel (refereegranskat)abstract
- Several studies have examined the functions of nucleic acids in small extracellular vesicles (sEVs). However, much less is known about the protein cargos of sEVs and their functions in recipient cells. This study demonstrates the presence of lysine-specific demethylase 1 (LSD1), which is the first identified histone demethylase, in the culture medium of gastric cancer cells. We show that sEVs derived from gastric cancer cells and the plasma of patients with gastric cancer harbor LSD1. The shuttling of LSD1-containing sEVs from donor cells to recipient gastric cancer cells promotes cancer cell stemness by positively regulating the expression of Nanog, OCT4, SOX2, and CD44. Additionally, sEV-delivered LSD1 suppresses oxaliplatin response of recipient cells in vitro and in vivo, whereas LSD1-depleted sEVs do not. Taken together, we demonstrate that LSD1-loaded sEVs can promote stemness and chemoresistance to oxaliplatin. These findings suggest that the LSD1 content of sEV could serve as a biomarker to predict oxaliplatin response in gastric cancer patients.
|
|
| 3. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 4. |
- Li, Xiao-Fei, et al.
(författare)
-
Unraveling the formation mechanism of graphitic nitrogen-doping in thermally treated graphene with ammonia
- 2016
-
Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 6
-
Tidskriftsartikel (refereegranskat)abstract
- Nitrogen-doped graphene (N-graphene) has attractive properties that has been widely studied over the years. However, its possible formation process still remains unclear. Here, we propose a highly feasible formation mechanism of the graphitic-N doing in thermally treated graphene with ammonia by performing ab initio molecular dynamic simulations at experimental conditions. Results show that among the commonly native point defects in graphene, only the single vacancy 5-9 and divacancy 555-777 have the desirable electronic structures to trap N-containing groups and to mediate the subsequent dehydrogenation processes. The local structure of the defective graphene in combining with the thermodynamic and kinetic effect plays a crucial role in dominating the complex atomic rearrangement to form graphitic-N which heals the corresponding defect perfectly. The importance of the symmetry, the localized force field, the interaction of multiple trapped N-containing groups, as well as the catalytic effect of the temporarily formed bridge-N are emphasized, and the predicted doping configuration agrees well with the experimental observation. Hence, the revealed mechanism will be helpful for realizing the targeted synthesis of N-graphene with reduced defects and desired properties.
|
|
| 5. |
- Lin, Xian Qi, et al.
(författare)
-
Electromagnetically induced transparencies in a closed waveguide with high efficiency and wide frequency band
- 2012
-
Ingår i: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 101:9, s. 093502-
-
Tidskriftsartikel (refereegranskat)abstract
- We propose an effective method to alleviate the conflict between large group delay and wide frequency band in electromagnetically induced transparency (EIT) design. Two kinds of electrically induced transparency (E-IT) and magnetically induced transparency (M-IT) are proposed, respectively. Then an EIT with wide transparency window is developed by combining the independent E-IT and M-IT structures. All of the transparencies are designed in a closed waveguide system, and high efficiency is obtained at the same time. One sample is fabricated and the bandwidth is increased to more than 3 times without decrease of the group delay and increase of the size.
|
|
| 6. |
- Lin, Xian Qi, et al.
(författare)
-
Understand and realize an "invisible gateway" in a classical way
- 2013
-
Ingår i: Progress In Electromagnetics Research. - 1070-4698 .- 1559-8985. ; 141, s. 739-749
-
Tidskriftsartikel (refereegranskat)abstract
- We create an invisible gateway simply by putting electric and magnetic superscatterers in a metallic waveguide. The characteristics of the electric and magnetic resonators are analyzed in a metallic hollow waveguide, and the dual-mode superscattering property is discussed in detail to broaden the bandwidth of the invisible gateway. Good agreement is achieved between the simulation and measurement for such an invisible gateway. The present work help readers understand easily how an invisible gateway works (or makes sense) in a classical way without using any complex metamaterial or complicated method of transformation optics.
|
|
| 7. |
- Qi, Jun, et al.
(författare)
-
IoTBDH-2023: The 5th International Workshop on Internet of Things of Big Data for Healthcare
- 2023
-
Ingår i: CIKM 2023 - Proceedings of the 32nd ACM International Conference on Information and Knowledge Management. - : Association for Computing Machinery. ; , s. 5285-5288
-
Konferensbidrag (refereegranskat)abstract
- Internet of Things (IoT) enabled technology has rapidly and efficiently facilitate healthcare diagnose and treatment with low-cost and lightweight devices. Big data generated from IoT offers valuable and crucial information to guide decision-making, improve patient outcomes, and decrease healthcare costs, etc. The workshop is aiming to provide an opportunity for researchers and practitioners from both academia and industry to present the state-of-the-art research and applications in utilizing IoT and big data technology for healthcare by presenting efficient scientific and engineering solutions, addressing the needs and challenges for integration with new technologies, and providing visions for future research and development.
|
|
| 8. |
- Wang, Lu, et al.
(författare)
-
The geographical distribution of grey wolves (Canis lupus) in China : a systematic review
- 2016
-
Ingår i: Zoological Research. - : Science Press. - 2095-8137. ; 37:6, s. 315-326
-
Forskningsöversikt (refereegranskat)abstract
- The grey wolf (Canis lupus) is one of the most widely distributed terrestrial mammals, and its distribution and ecology in Europe and North America are largely well described. However, the distribution of grey wolves in southern China is still highly controversial. Several well-known western literatures stated that there were no grey wolves in southern China, while the presence of grey wolves across China has been indicated in A Guide to the Mammals of China, published by Princeton University Press. It is essential to solve this discrepancy since dogs may have originated from grey wolves in southern China. Therefore, we systematically investigated Chinese literatures about wild animal surveys and identified more than 100 articles and books that included information of the distribution of grey wolves in China. We also surveyed the collections of three Chinese natural museums and found 26 grey wolf skins specimens collected across China. Moreover, we investigated the fossil records in China and identified 25 archaeological sites with wolf remains including south China. In conclusion, with the comprehensive summary of Chinese literatures, museum specimens and fossil records, we demonstrate that grey wolves do distribute across all parts of the Chinese mainland, including the most southern parts.
|
|
