Sökning: WFRF:(Qundos Ulrika)
> Ayoglu Burcu >
Antibody-based prof...
Antibody-based profiling of cerebrospinal fluid within multiple sclerosis
-
- Häggmark, Anna (författare)
- KTH,Science for Life Laboratory, SciLifeLab
-
- Byström, Sanna (författare)
- KTH,Science for Life Laboratory, SciLifeLab
-
- Ayoglu, Burcu (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
visa fler...
-
- Qundos, Ulrika (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
- Uhlén, Mathias (författare)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
-
- Khademi, M. (författare)
- Karolinska Institutet
-
- Olsson, T. (författare)
- Karolinska Institutet
-
- Schwenk, Jochen M. (författare)
- KTH,Science for Life Laboratory, SciLifeLab
-
- Nilsson, Peter (författare)
- KTH,Science for Life Laboratory, SciLifeLab
-
visa färre...
-
(creator_code:org_t)
- 2013-06-25
- 2013
- Engelska.
-
Ingår i: Proteomics. - : Wiley. - 1615-9853 .- 1615-9861. ; 13:15, s. 2256-2267
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Antibody suspension bead arrays have proven to enable multiplexed and high-throughput protein profiling in unfractionated plasma and serum samples through a direct labeling approach. We here describe the development and application of an assay for protein profiling of cerebrospinal fluid (CSF). While setting up the assay, systematic intensity differences between sample groups were observed that reflected inherent sample specific total protein amounts. Supplementing the labeling reaction with BSA and IgG diminished these differences without impairing the apparent sensitivity of the assay. We also assessed the effects of heat treatment on the analysis of CSF proteins and applied the assay to profile 43 selected proteins by 101 antibodies in 339 CSF samples from a multiple sclerosis (MS) cohort. Two proteins, GAP43 and SERPINA3 were found to have a discriminating potential with altered intensity levels between sample groups. GAP43 was detected at significantly lower levels in secondary progressive MS compared to early stages of MS and the control group of other neurological diseases. SERPINA3 instead was detected at higher levels in all MS patients compared to controls. The developed assay procedure now offers new possibilities for broad-scale protein profiling of CSF within neurological disorders.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Antibody microarrays
- Biomarker discovery
- Cerebrospinal fluid
- Multiplexed proteomics technology
- Protein arrays
- Proteome profiling
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas