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Ancient bacteria show evidence of DNA repair

Johnson, Sarah Stewart (author)
Hebsgaard, Martin B. (author)
Christensen, Torben (author)
Lund University,Lunds universitet,Institutionen för naturgeografi och ekosystemvetenskap,Naturvetenskapliga fakulteten,Dept of Physical Geography and Ecosystem Science,Faculty of Science
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Mastepanov, Mikhail (author)
Lund University,Lunds universitet,Institutionen för naturgeografi och ekosystemvetenskap,Naturvetenskapliga fakulteten,Dept of Physical Geography and Ecosystem Science,Faculty of Science
Nielsen, Rasmus (author)
Munch, Kasper (author)
Brand, Tina (author)
Thomas, M. (author)
Gilbert, P. (author)
Zuber, Maria T. (author)
Bunce, Michael (author)
Ronn, Regin (author)
Gilichinsky, David (author)
Froese, Duane (author)
Willerslev, Eske (author)
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 (creator_code:org_t)
2007-09-04
2007
English.
In: Proceedings of the National Academy of Sciences. - : Proceedings of the National Academy of Sciences. - 1091-6490 .- 0027-8424. ; 104:36, s. 14401-14405
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Recent claims of cultivable ancient bacteria within sealed environments highlight our limited understanding of the mechanisms behind long-term cell survival. It remains unclear how dormancy, a favored explanation for extended cellular persistence, can cope with spontaneous genomic decay over geological timescales. There has been no direct evidence in ancient microbes for the most likely mechanism, active DNA repair, or for the metabolic activity necessary to sustain it. In this paper, we couple PCR and enzymatic treatment of DNA with direct respiration measurements to investigate long-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence of bacterial survival in samples up to half a million years in age, making this the oldest independently authenticated DNA to date obtained from viable cells. Additionally, we find strong evidence that this long-term survival is closely tied to cellular metabolic activity and DNA repair that over time proves to be superior to dormancy as a mechanism in sustaining bacteria viability.

Subject headings

NATURVETENSKAP  -- Geovetenskap och miljövetenskap -- Naturgeografi (hsv//swe)
NATURAL SCIENCES  -- Earth and Related Environmental Sciences -- Physical Geography (hsv//eng)

Keyword

DNA damage
long-term microbial survival
metabolic activity

Publication and Content Type

art (subject category)
ref (subject category)

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