| 1. |
- Huang, Yanqin, et al.
(författare)
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Humic Acids Combined with Dairy Slurry as Fertilizer Can Increase Alfalfa Yield and Reduce Nitrogen Losses
- 2024
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Ingår i: Agriculture. - : Multidisciplinary Digital Publishing Institute (MDPI). - 2077-0472. ; 14:8
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Tidskriftsartikel (refereegranskat)abstract
- Dairy slurry could be a significant source of nitrogen (N) for plants, but mismanagement can lead to atmospheric ammonia losses or nitrate leaching into groundwater. To make the use of dairy slurry efficient and reasonable, the loss of N pollution to the environment should be reduced. We used repacked lysimeters to comprehensively determine ammonia emission and N leaching losses in an alfalfa–soil system. The application of dairy slurry had no significant effect on alfalfa yield at the same rate of N application in comparison to chemical fertilizer, and adding humic acids significantly increased yield by about 12%. However, the application of dairy slurry increased the ammonia emission rate significantly, leading to an increase in the cumulative amount of ammonia emission, while the addition of humic acids reduced the ammonia emissions by 11%. Chemical fertilizer and dairy slurry application significantly increased nitrate leaching compared to the control treatment, while the addition of humic acids can significantly reduce ammonium N leaching. Dairy slurry was proven to be as effective as chemical N fertilizer in achieving the optimum biomass, and adding humic acids can significantly reduce N loss to the atmosphere and groundwater. This study showed the possibility of replacing chemical fertilizer with dairy slurry in alfalfa production and the advantages of humic acids’ addition to alfalfa to maintain production yield and improve environmental friendliness.
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| 2. |
- Rozenbaum, Rene, et al.
(författare)
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Antimicrobial synergy of monolaurin lipid nanocapsules with adsorbed antimicrobial peptides against Staphylococcus aureus biofilms in vitro is absent in vivo
- 2019
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Ingår i: Journal of Controlled Release. - : Elsevier BV. - 0168-3659 .- 1873-4995. ; 293, s. 73-83
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Tidskriftsartikel (refereegranskat)abstract
- Bacterial infections are mostly due to bacteria in their biofilm-mode of growth, while penetrability of antimicrobials into infectious biofilms and increasing antibiotic resistance hamper infection treatment. In-vitro, monolaurin lipid nanocapsules (ML-LNCs) carrying adsorbed antimicrobial peptides (AMPs) displayed synergistic efficacy against planktonic Staphylococcus aureus, but it has not been demonstrated, neither in-vitro nor in-vivo, that such ML-LNCs penetrate into infectious S. aureus biofilms and maintain synergy with AMPs. This study investigates the release mechanism of AMPs from ML-LNCs and possible antimicrobial synergy of ML-LNCs with the AMPs DPK-060 and LL-37 against S. aureus biofilms in-vitro and in a therapeutic, murine, infected wound-healing model. Zeta potentials demonstrated that AMP release from ML-LNCs was controlled by the AMP concentration in suspension. Both AMPs demonstrated no antimicrobial efficacy against four staphylococcal strains in a planktonic mode, while a checkerboard assay showed synergistic antimicrobial efficacy when ML-LNCs and DPK-060 were combined, but not for combinations of ML-LNCs and LL-37. Similar effects were seen for growth reduction of staphylococcal biofilms, with antimicrobial synergy persisting only for ML-LNCs at the highest level of DPK-060 or LL-37 adsorption. Healing of wounds infected with bioluminescent S. aureus Xen36, treated with ML-LNCs alone, was faster when treated with PBS, while AMPs alone did not yield faster wound-healing than PBS. Faster, synergistic wound-healing due to ML-LNCs with adsorbed DPK-060, was absent in-vivo. Summarizing, antimicrobial synergy of ML-LNCs with adsorbed antimicrobial peptides as seen in-vitro, is absent in in-vivo healing of infected wounds, likely because host AMPs adapted the synergistic role of the AMPs added. Thus, conclusions regarding synergistic antimicrobial efficacy, should not be drawn from planktonic data, while even in-vitro biofilm data bear little relevance for the in-vivo situation. © 2018
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| 3. |
- Xi, Fengming, et al.
(författare)
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Substantial global carbon uptake by cement carbonation
- 2016
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Ingår i: Nature Geoscience. - : Springer Science and Business Media LLC. - 1752-0894 .- 1752-0908. ; 9:12, s. 880-883
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Tidskriftsartikel (refereegranskat)abstract
- Calcination of carbonate rocks during the manufacture of cement produced 5% of global CO 2 emissions from all industrial process and fossil-fuel combustion in 2013. Considerable attention has been paid to quantifying these industrial process emissions from cement production, but the natural reversal of the process - carbonation - has received little attention in carbon cycle studies. Here, we use new and existing data on cement materials during cement service life, demolition, and secondary use of concrete waste to estimate regional and global CO 2 uptake between 1930 and 2013 using an analytical model describing carbonation chemistry. We find that carbonation of cement materials over their life cycle represents a large and growing net sink of CO 2, increasing from 0.10 GtC yr â '1 in 1998 to 0.25 GtC yr â '1 in 2013. In total, we estimate that a cumulative amount of 4.5 GtC has been sequestered in carbonating cement materials from 1930 to 2013, offsetting 43% of the CO 2 emissions from production of cement over the same period, not including emissions associated with fossil use during cement production. We conclude that carbonation of cement products represents a substantial carbon sink that is not currently considered in emissions inventories.
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