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Sökning: WFRF:(Shi Xiao ming) > Kungliga Tekniska Högskolan

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Wang, Kewei, et al. (författare)
  • Transmit/Passive Beamforming Design for Multi-IRS Assisted Cell-Free MIMO Networks
  • 2023
  • Ingår i: IEEE Systems Journal. - : Institute of Electrical and Electronics Engineers (IEEE). - 1932-8184 .- 1937-9234. ; 17:4, s. 6282-6291
  • Tidskriftsartikel (refereegranskat)abstract
    • For beyond fifth-generation and sixth-generation communication, intelligent reflecting surface (IRS)/reconfigurable intelligent surface, and cell-free networks have been proposed as revolutionary technologies, which could significantly improve the connection quality and coverage to meet future communication demands for extremely massive connectivity and high reliability. In this article, a transmit/passive beamforming strategy for multi-IRS assisted cell-free multi-input-multi-output network is proposed to maximize the weighted sum-rate (WSR). Specifically, for passive beamforming of IRS, an elementwise block coordinate descent framework is applied to efficiently solve the unit-modulus constraints and reduce computation complexity greatly. Note that, every variable in our proposed algorithm can be updated with closed-form solutions and our algorithm can be applied with distributed implementation. Both analytical and simulation results demonstrate that, while guaranteeing superiority in WSR performance and information interaction cost, the proposed scheme outperforms the benchmark algorithms with closed-form solutions significantly in terms of complexity cost.
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  • Resultat 1-10 av 85

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